Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis
Stylo (Stylosanthes guianensis) is a tropical legume known for its exceptional tolerance to low phosphate (Pi), a trait believed to be linked to its high acid phosphatase (APase) activity. Previous studies have observed genotypic variations in APase activity in stylo; however, the gene encoding the...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/134939 |
| _version_ | 1855527338519822336 |
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| author | Luo, Jiajia Chen, Zhijian Huang, Rui Wu, Yuanhang Liu, Chun Cai, Zeping Dong, Rongshu Arango, Jacobo Rao, Idupulapati M. Schultze-Kraft, Rainer Liu, Guodao Liu, Pandao |
| author_browse | Arango, Jacobo Cai, Zeping Chen, Zhijian Dong, Rongshu Huang, Rui Liu, Chun Liu, Guodao Liu, Pandao Luo, Jiajia Rao, Idupulapati M. Schultze-Kraft, Rainer Wu, Yuanhang |
| author_facet | Luo, Jiajia Chen, Zhijian Huang, Rui Wu, Yuanhang Liu, Chun Cai, Zeping Dong, Rongshu Arango, Jacobo Rao, Idupulapati M. Schultze-Kraft, Rainer Liu, Guodao Liu, Pandao |
| author_sort | Luo, Jiajia |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Stylo (Stylosanthes guianensis) is a tropical legume known for its exceptional tolerance to low phosphate (Pi), a trait believed to be linked to its high acid phosphatase (APase) activity. Previous studies have observed genotypic variations in APase activity in stylo; however, the gene encoding the crucial APase responsible for this variation remains unidentified. In this study, transcriptomic and proteomic analyses were employed to identify eight Pi starvation‐inducible (PSI) APases belonging to the purple APase (PAP) family in the roots of stylo and seven in the leaves. Among these PSI‐PAPs, SgPAP7 exhibited a significantly positive correlation in its expression levels with the activities of both internal APase and root‐associated APase across 20 stylo genotypes under low‐Pi conditions. Furthermore, the recombinant SgPAP7 displayed high catalytic activity toward adenosine 5′‐diphosphate (ADP) and phosphoenolpyruvate (PEP) in vitro. Overexpression (OE) of SgPAP7 in Arabidopsis facilitated exogenous organic phosphorus utilization. Moreover, SgPAP7 OE lines showed lower shoot ADP and PEP levels than the wild type, implying that SgPAP7 is involved in the catabolism and recycling of endogenous ADP and PEP, which could be beneficial for plant growth in low‐Pi soils. In conclusion, SgPAP7 is a key gene with a major role in stylo adaptation to low‐Pi conditions by facilitating the utilization of both exogenous and endogenous organic phosphorus sources. It may also function as a PEP phosphatase involved in a glycolytic bypass pathway that minimizes the need for adenylates and Pi. Thus, SgPAP7 could be a promising target for improving tolerance of crops to low‐Pi availability. |
| format | Journal Article |
| id | CGSpace134939 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1349392025-12-12T14:51:54Z Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis Luo, Jiajia Chen, Zhijian Huang, Rui Wu, Yuanhang Liu, Chun Cai, Zeping Dong, Rongshu Arango, Jacobo Rao, Idupulapati M. Schultze-Kraft, Rainer Liu, Guodao Liu, Pandao phosphorus uses proteomics transcriptomics analysis tropical legumes stylosanthes guianensis Stylo (Stylosanthes guianensis) is a tropical legume known for its exceptional tolerance to low phosphate (Pi), a trait believed to be linked to its high acid phosphatase (APase) activity. Previous studies have observed genotypic variations in APase activity in stylo; however, the gene encoding the crucial APase responsible for this variation remains unidentified. In this study, transcriptomic and proteomic analyses were employed to identify eight Pi starvation‐inducible (PSI) APases belonging to the purple APase (PAP) family in the roots of stylo and seven in the leaves. Among these PSI‐PAPs, SgPAP7 exhibited a significantly positive correlation in its expression levels with the activities of both internal APase and root‐associated APase across 20 stylo genotypes under low‐Pi conditions. Furthermore, the recombinant SgPAP7 displayed high catalytic activity toward adenosine 5′‐diphosphate (ADP) and phosphoenolpyruvate (PEP) in vitro. Overexpression (OE) of SgPAP7 in Arabidopsis facilitated exogenous organic phosphorus utilization. Moreover, SgPAP7 OE lines showed lower shoot ADP and PEP levels than the wild type, implying that SgPAP7 is involved in the catabolism and recycling of endogenous ADP and PEP, which could be beneficial for plant growth in low‐Pi soils. In conclusion, SgPAP7 is a key gene with a major role in stylo adaptation to low‐Pi conditions by facilitating the utilization of both exogenous and endogenous organic phosphorus sources. It may also function as a PEP phosphatase involved in a glycolytic bypass pathway that minimizes the need for adenylates and Pi. Thus, SgPAP7 could be a promising target for improving tolerance of crops to low‐Pi availability. 2024-02 2023-12-04T10:18:29Z 2023-12-04T10:18:29Z Journal Article https://hdl.handle.net/10568/134939 en Open Access application/pdf Wiley Luo, J.; Chen, Z.; Huang, R.; Wu, Y.; Liu, C.; Cai, Z.; Dong, R.; Arango, J.; Rao, I.M.; Schultze-Kraft, R.; Liu, G. and Liu, P. 2024. Multi-omics analysis reveals the roles of purple acid phosphatases in or-ganic phosphorus utilization by the tropical legume Stylosanthes guianensis. The Plant Journal 117(3):729-746. ISSN: 1365-313X |
| spellingShingle | phosphorus uses proteomics transcriptomics analysis tropical legumes stylosanthes guianensis Luo, Jiajia Chen, Zhijian Huang, Rui Wu, Yuanhang Liu, Chun Cai, Zeping Dong, Rongshu Arango, Jacobo Rao, Idupulapati M. Schultze-Kraft, Rainer Liu, Guodao Liu, Pandao Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis |
| title | Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis |
| title_full | Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis |
| title_fullStr | Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis |
| title_full_unstemmed | Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis |
| title_short | Multi-omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume Stylosanthes guianensis |
| title_sort | multi omics analysis reveals the roles of purple acid phosphatases in organic phosphorus utilization by the tropical legume stylosanthes guianensis |
| topic | phosphorus uses proteomics transcriptomics analysis tropical legumes stylosanthes guianensis |
| url | https://hdl.handle.net/10568/134939 |
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