The hypervariable Tpr multigene family of Theileria parasites, defined by a conserved, membrane-associated, C-terminal domain, includes several copies with defined orthology between species
Multigene families often play an important role in host-parasite interactions. One of the largest multigene families in Theileria parva, the causative agent of East Coast fever, is the T. parva repeat (Tpr) gene family. The function of the putative Tpr proteins remains unknown. The initial publicati...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/134837 |
| _version_ | 1855541504368443392 |
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| author | Palmateer, N.C. Munro, James B. Nagaraj, S. Crabtree, J. Pelle, Roger Tallon, L. Nene, Vishvanath M. Bishop, Richard P. Silva, J.C. |
| author_browse | Bishop, Richard P. Crabtree, J. Munro, James B. Nagaraj, S. Nene, Vishvanath M. Palmateer, N.C. Pelle, Roger Silva, J.C. Tallon, L. |
| author_facet | Palmateer, N.C. Munro, James B. Nagaraj, S. Crabtree, J. Pelle, Roger Tallon, L. Nene, Vishvanath M. Bishop, Richard P. Silva, J.C. |
| author_sort | Palmateer, N.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Multigene families often play an important role in host-parasite interactions. One of the largest multigene families in Theileria parva, the causative agent of East Coast fever, is the T. parva repeat (Tpr) gene family. The function of the putative Tpr proteins remains unknown. The initial publication of the T. parva reference genome identified 39 Tpr family open reading frames (ORFs) sharing a conserved C-terminal domain. Twenty-eight of these are clustered in a central region of chromosome 3, termed the “Tpr locus”, while others are dispersed throughout all four nuclear chromosomes. The Tpr locus contains three of the four assembly gaps remaining in the genome, suggesting the presence of additional, as yet uncharacterized, Tpr gene copies. Here, we describe the use of long-read sequencing to attempt to close the gaps in the reference assembly of T. parva (located among multigene families clusters), characterize the full complement of Tpr family ORFs in the T. parva reference genome, and evaluate their evolutionary relationship with Tpr homologs in other Theileria species. We identify three new Tpr family genes in the T. parva reference genome and show that sequence similarity among paralogs in the Tpr locus is significantly higher than between genes outside the Tpr locus. We also identify sequences homologous to the conserved C-terminal domain in five additional Theileria species. Using these sequences, we show that the evolution of this gene family involves conservation of a few orthologs across species, combined with gene gains/losses, and species-specific expansions. |
| format | Journal Article |
| id | CGSpace134837 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1348372025-10-26T12:50:57Z The hypervariable Tpr multigene family of Theileria parasites, defined by a conserved, membrane-associated, C-terminal domain, includes several copies with defined orthology between species Palmateer, N.C. Munro, James B. Nagaraj, S. Crabtree, J. Pelle, Roger Tallon, L. Nene, Vishvanath M. Bishop, Richard P. Silva, J.C. genes east coast fever genomics Multigene families often play an important role in host-parasite interactions. One of the largest multigene families in Theileria parva, the causative agent of East Coast fever, is the T. parva repeat (Tpr) gene family. The function of the putative Tpr proteins remains unknown. The initial publication of the T. parva reference genome identified 39 Tpr family open reading frames (ORFs) sharing a conserved C-terminal domain. Twenty-eight of these are clustered in a central region of chromosome 3, termed the “Tpr locus”, while others are dispersed throughout all four nuclear chromosomes. The Tpr locus contains three of the four assembly gaps remaining in the genome, suggesting the presence of additional, as yet uncharacterized, Tpr gene copies. Here, we describe the use of long-read sequencing to attempt to close the gaps in the reference assembly of T. parva (located among multigene families clusters), characterize the full complement of Tpr family ORFs in the T. parva reference genome, and evaluate their evolutionary relationship with Tpr homologs in other Theileria species. We identify three new Tpr family genes in the T. parva reference genome and show that sequence similarity among paralogs in the Tpr locus is significantly higher than between genes outside the Tpr locus. We also identify sequences homologous to the conserved C-terminal domain in five additional Theileria species. Using these sequences, we show that the evolution of this gene family involves conservation of a few orthologs across species, combined with gene gains/losses, and species-specific expansions. 2023-12 2023-11-29T12:21:55Z 2023-11-29T12:21:55Z Journal Article https://hdl.handle.net/10568/134837 en Open Access Springer Palmateer, N.C., Munro, J.B., Nagaraj, S., Crabtree, J., Pelle, R., Tallon, L., Nene, V., Bishop, R. and Silva, J.C. 2023. The hypervariable Tpr multigene family of Theileria parasites, defined by a conserved, membrane-associated, C-terminal domain, includes several copies with defined orthology between species. Journal of Molecular Evolution 91(6): 897–911. |
| spellingShingle | genes east coast fever genomics Palmateer, N.C. Munro, James B. Nagaraj, S. Crabtree, J. Pelle, Roger Tallon, L. Nene, Vishvanath M. Bishop, Richard P. Silva, J.C. The hypervariable Tpr multigene family of Theileria parasites, defined by a conserved, membrane-associated, C-terminal domain, includes several copies with defined orthology between species |
| title | The hypervariable Tpr multigene family of Theileria parasites, defined by a conserved, membrane-associated, C-terminal domain, includes several copies with defined orthology between species |
| title_full | The hypervariable Tpr multigene family of Theileria parasites, defined by a conserved, membrane-associated, C-terminal domain, includes several copies with defined orthology between species |
| title_fullStr | The hypervariable Tpr multigene family of Theileria parasites, defined by a conserved, membrane-associated, C-terminal domain, includes several copies with defined orthology between species |
| title_full_unstemmed | The hypervariable Tpr multigene family of Theileria parasites, defined by a conserved, membrane-associated, C-terminal domain, includes several copies with defined orthology between species |
| title_short | The hypervariable Tpr multigene family of Theileria parasites, defined by a conserved, membrane-associated, C-terminal domain, includes several copies with defined orthology between species |
| title_sort | hypervariable tpr multigene family of theileria parasites defined by a conserved membrane associated c terminal domain includes several copies with defined orthology between species |
| topic | genes east coast fever genomics |
| url | https://hdl.handle.net/10568/134837 |
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