Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue
This report presents the detailed protocol that we used for our first attempt in measuring ploidy using fresh leaf tissue in Interspecific Brachiaria using the sysmex flow cytometer with DAPI cystain UV stain. Accurate determination of ploidy is crucial for understanding the genetic characteristics...
| Autores principales: | , , |
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| Formato: | Informe técnico |
| Lenguaje: | Español |
| Publicado: |
International Center for Tropical Agriculture
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/134766 |
| _version_ | 1855538282337665024 |
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| author | Perea, Claudia Samantha Hernández, Diana Jauregui, Rosa |
| author_browse | Hernández, Diana Jauregui, Rosa Perea, Claudia Samantha |
| author_facet | Perea, Claudia Samantha Hernández, Diana Jauregui, Rosa |
| author_sort | Perea, Claudia Samantha |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This report presents the detailed protocol that we used for our first attempt in measuring ploidy using fresh leaf tissue in Interspecific Brachiaria using the sysmex flow cytometer with DAPI cystain UV stain. Accurate determination of ploidy is crucial for understanding the genetic characteristics of plant populations, aiding in plant breeding programs, and contributing to advancements in molecular biology. The following methodology outlines the steps for sample collection, preparation, staining, and analysis.
This protocol comes from the training in the sysmex flow cytometer on 5/11/202 in CIAT- headquarters by the company representatives. Although preliminary results from the technique are inconclusive, mostly because of technical difficulties with the flow cytometer, this is a first approach to standardize the protocol and it will give us insights in how to proceed next year. |
| format | Informe técnico |
| id | CGSpace134766 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace1347662025-11-05T12:33:47Z Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue Protocolo para medir la ploidía en tejido de hojas frescas interespecíficas de Brachiaria Perea, Claudia Samantha Hernández, Diana Jauregui, Rosa measurement genetic markers marcadores genéticos urochloa This report presents the detailed protocol that we used for our first attempt in measuring ploidy using fresh leaf tissue in Interspecific Brachiaria using the sysmex flow cytometer with DAPI cystain UV stain. Accurate determination of ploidy is crucial for understanding the genetic characteristics of plant populations, aiding in plant breeding programs, and contributing to advancements in molecular biology. The following methodology outlines the steps for sample collection, preparation, staining, and analysis. This protocol comes from the training in the sysmex flow cytometer on 5/11/202 in CIAT- headquarters by the company representatives. Although preliminary results from the technique are inconclusive, mostly because of technical difficulties with the flow cytometer, this is a first approach to standardize the protocol and it will give us insights in how to proceed next year. 2023-11 2023-11-28T17:26:28Z 2023-11-28T17:26:28Z Report https://hdl.handle.net/10568/134766 es Open Access application/pdf International Center for Tropical Agriculture Perea, C.; Hernández, D.; Jauregui, R. (2023) Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Measuring ploidy in Interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue. Cali (Colombia): Centro Internacional de Agricultura Tropical (CIAT). 15 p. |
| spellingShingle | measurement genetic markers marcadores genéticos urochloa Perea, Claudia Samantha Hernández, Diana Jauregui, Rosa Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue |
| title | Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue |
| title_full | Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue |
| title_fullStr | Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue |
| title_full_unstemmed | Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue |
| title_short | Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue |
| title_sort | measuring ploidy in interspecific urochloa syn brachiaria using sysmex cyflow cytometer with dapi cystain uv stain protocol for measuring ploidy in interspecific brachiaria fresh leaf tissue |
| topic | measurement genetic markers marcadores genéticos urochloa |
| url | https://hdl.handle.net/10568/134766 |
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