Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions
Seed deterioration during storage results in poor germination, reduced vigour, and non-uniform seedling emergence. The aging rate depends on storage conditions and genetic factors. This study aims to identify these genetic factors determining the longevity of rice (Oryza sativa L.) seeds stored unde...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/132730 |
| _version_ | 1855520399977086976 |
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| author | Prasad C.T., Manjunath Kodde, Jan Angenent, Gerco C. Hay, Fiona R. McNally, Kenneth L. Groot, Steven P.C. |
| author_browse | Angenent, Gerco C. Groot, Steven P.C. Hay, Fiona R. Kodde, Jan McNally, Kenneth L. Prasad C.T., Manjunath |
| author_facet | Prasad C.T., Manjunath Kodde, Jan Angenent, Gerco C. Hay, Fiona R. McNally, Kenneth L. Groot, Steven P.C. |
| author_sort | Prasad C.T., Manjunath |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Seed deterioration during storage results in poor germination, reduced vigour, and non-uniform seedling emergence. The aging rate depends on storage conditions and genetic factors. This study aims to identify these genetic factors determining the longevity of rice (Oryza sativa L.) seeds stored under experimental aging conditions mimicking long-term dry storage. Genetic variation for tolerance to aging was studied in 300 Indica rice accessions by storing dry seeds under an elevated partial pressure of oxygen (EPPO) condition. A genome-wide association analysis identified 11 unique genomic regions for all measured germination parameters after aging, differing from those previously identified in rice under humid experimental aging conditions. The significant single nucleotide polymorphism in the most prominent region was located within the Rc gene, encoding a basic helix-loop-helix transcription factor. Storage experiments using near-isogenic rice lines (SD7-1D (Rc) and SD7-1d (rc) with the same allelic variation confirmed the role of the wildtype Rc gene, providing stronger tolerance to dry EPPO aging. In the seed pericarp, a functional Rc gene results in accumulation of proanthocyanidins, an important sub-class of flavonoids having strong antioxidant activity, which may explain the variation in tolerance to dry EPPO aging. |
| format | Journal Article |
| id | CGSpace132730 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1327302025-11-12T04:56:25Z Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions Prasad C.T., Manjunath Kodde, Jan Angenent, Gerco C. Hay, Fiona R. McNally, Kenneth L. Groot, Steven P.C. storage conditions germination seedling emergence genetic variation single nucleotide polymorphism allelic variation flavonoids hybrid vigour keeping quality Seed deterioration during storage results in poor germination, reduced vigour, and non-uniform seedling emergence. The aging rate depends on storage conditions and genetic factors. This study aims to identify these genetic factors determining the longevity of rice (Oryza sativa L.) seeds stored under experimental aging conditions mimicking long-term dry storage. Genetic variation for tolerance to aging was studied in 300 Indica rice accessions by storing dry seeds under an elevated partial pressure of oxygen (EPPO) condition. A genome-wide association analysis identified 11 unique genomic regions for all measured germination parameters after aging, differing from those previously identified in rice under humid experimental aging conditions. The significant single nucleotide polymorphism in the most prominent region was located within the Rc gene, encoding a basic helix-loop-helix transcription factor. Storage experiments using near-isogenic rice lines (SD7-1D (Rc) and SD7-1d (rc) with the same allelic variation confirmed the role of the wildtype Rc gene, providing stronger tolerance to dry EPPO aging. In the seed pericarp, a functional Rc gene results in accumulation of proanthocyanidins, an important sub-class of flavonoids having strong antioxidant activity, which may explain the variation in tolerance to dry EPPO aging. 2023-06 2023-11-06T01:42:23Z 2023-11-06T01:42:23Z Journal Article https://hdl.handle.net/10568/132730 en Open Access application/pdf Wiley Prasad CT, Manjunath, Jan Kodde, Gerco C. Angenent, Fiona R. Hay, Kenneth L. McNally, and Steven PC Groot. "Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions." Plant, Cell & Environment 46, no. 6 (2023): 1962-1980. |
| spellingShingle | storage conditions germination seedling emergence genetic variation single nucleotide polymorphism allelic variation flavonoids hybrid vigour keeping quality Prasad C.T., Manjunath Kodde, Jan Angenent, Gerco C. Hay, Fiona R. McNally, Kenneth L. Groot, Steven P.C. Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions |
| title | Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions |
| title_full | Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions |
| title_fullStr | Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions |
| title_full_unstemmed | Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions |
| title_short | Identification of the rice Rc gene as a main regulator of seed survival under dry storage conditions |
| title_sort | identification of the rice rc gene as a main regulator of seed survival under dry storage conditions |
| topic | storage conditions germination seedling emergence genetic variation single nucleotide polymorphism allelic variation flavonoids hybrid vigour keeping quality |
| url | https://hdl.handle.net/10568/132730 |
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