qSUB2: A novel QTL with positive epistasis with SUB1 locus enhances submergence tolerance in rice

Climate change is expected to significantly increase submergence stress of lowland rice (Oryza sativa) production in Africa. There is a need to further improve the ability of SUB1 gene to tolerate submergence stress. Genetic control of submergence tolerance was studied in a moderately tolerant to su...

Descripción completa

Detalles Bibliográficos
Autores principales: Akintayo, O., Daniel, I., Afeez, S., Jolayemi, O., Semwal, V., Venuprasad, R.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 2023
Materias:
Acceso en línea:https://hdl.handle.net/10568/132705
_version_ 1855537057838923776
author Akintayo, O.
Daniel, I.
Afeez, S.
Jolayemi, O.
Semwal, V.
Venuprasad, R.
author_browse Afeez, S.
Akintayo, O.
Daniel, I.
Jolayemi, O.
Semwal, V.
Venuprasad, R.
author_facet Akintayo, O.
Daniel, I.
Afeez, S.
Jolayemi, O.
Semwal, V.
Venuprasad, R.
author_sort Akintayo, O.
collection Repository of Agricultural Research Outputs (CGSpace)
description Climate change is expected to significantly increase submergence stress of lowland rice (Oryza sativa) production in Africa. There is a need to further improve the ability of SUB1 gene to tolerate submergence stress. Genetic control of submergence tolerance was studied in a moderately tolerant to submergence African rice landrace “TOS6454.” Three mapping populations with a total of 1182 lines were developed by crossing TOS6454 with three popular rice varieties of West Africa that are high-yielding but submergence susceptible, that is, FARO44, FARO52, and FARO60. The screening for submergence was conducted at the Africa Rice Center (AfricaRice) station in Ibadan, Nigeria during 2016–2018. The three mapping populations were genotyped with single nucleotide polymorphic markers either on the Kompetitive allele specific PCR or the DArTseq platforms. Quantitative trait locus (QTL) analysis revealed five different QTLs on chromosomes 1, 2, 3, 4, and 9. The QTLs on chromosomes 2 (qSUB2) and 9 (qSUB9) had consistent large effects and the tolerance alleles were contributed by TOS6454. The QTL on chromosome 9 (qSUB9) had a large effect on submergence tolerance in all three mapping populations. This QTL had a highest logarithm of odds (LOD) score of 30.46 and explained as much as 27.7% phenotypic variation (R2), with an additive effect of up to 9.39. The QTL on chromosome 2 (qSUB2) had a highest LOD score of 10.78 and explained up to 10.8% R2 for this trait. Furthermore, qSUB2 had positive epistasis with qSUB9 which further increased submergence tolerance. A total of 57 lines carrying both qSUB2 and qSUB9 alleles were superior to all other lines in submergence tolerance. qSUB9 co-segregated with ART5 a marker for the SUB1 gene; thus, it is suggested that qSUB9 is the same as the well-known SUB1 gene. Pyramiding qSUB2 with the SUB1 gene locus is expected to further improve submergence tolerance in elite rice varieties.
format Journal Article
id CGSpace132705
institution CGIAR Consortium
language Inglés
publishDate 2023
publishDateRange 2023
publishDateSort 2023
publisher Wiley
publisherStr Wiley
record_format dspace
spelling CGSpace1327052025-12-08T09:54:28Z qSUB2: A novel QTL with positive epistasis with SUB1 locus enhances submergence tolerance in rice Akintayo, O. Daniel, I. Afeez, S. Jolayemi, O. Semwal, V. Venuprasad, R. genotyping tolerance rice Climate change is expected to significantly increase submergence stress of lowland rice (Oryza sativa) production in Africa. There is a need to further improve the ability of SUB1 gene to tolerate submergence stress. Genetic control of submergence tolerance was studied in a moderately tolerant to submergence African rice landrace “TOS6454.” Three mapping populations with a total of 1182 lines were developed by crossing TOS6454 with three popular rice varieties of West Africa that are high-yielding but submergence susceptible, that is, FARO44, FARO52, and FARO60. The screening for submergence was conducted at the Africa Rice Center (AfricaRice) station in Ibadan, Nigeria during 2016–2018. The three mapping populations were genotyped with single nucleotide polymorphic markers either on the Kompetitive allele specific PCR or the DArTseq platforms. Quantitative trait locus (QTL) analysis revealed five different QTLs on chromosomes 1, 2, 3, 4, and 9. The QTLs on chromosomes 2 (qSUB2) and 9 (qSUB9) had consistent large effects and the tolerance alleles were contributed by TOS6454. The QTL on chromosome 9 (qSUB9) had a large effect on submergence tolerance in all three mapping populations. This QTL had a highest logarithm of odds (LOD) score of 30.46 and explained as much as 27.7% phenotypic variation (R2), with an additive effect of up to 9.39. The QTL on chromosome 2 (qSUB2) had a highest LOD score of 10.78 and explained up to 10.8% R2 for this trait. Furthermore, qSUB2 had positive epistasis with qSUB9 which further increased submergence tolerance. A total of 57 lines carrying both qSUB2 and qSUB9 alleles were superior to all other lines in submergence tolerance. qSUB9 co-segregated with ART5 a marker for the SUB1 gene; thus, it is suggested that qSUB9 is the same as the well-known SUB1 gene. Pyramiding qSUB2 with the SUB1 gene locus is expected to further improve submergence tolerance in elite rice varieties. 2023-05 2023-11-03T12:35:11Z 2023-11-03T12:35:11Z Journal Article https://hdl.handle.net/10568/132705 en Limited Access Wiley Akintayo, O. Daniel, I. Afeez, S. Jolayemi, O. Semwal, V. Venuprasad, R.qSUB2: A novel QTL with positive epistasis with SUB1 locus enhances submergence tolerance in rice.Crop science.2023, Volume 63, Issue 3:1246-1256.
spellingShingle genotyping
tolerance
rice
Akintayo, O.
Daniel, I.
Afeez, S.
Jolayemi, O.
Semwal, V.
Venuprasad, R.
qSUB2: A novel QTL with positive epistasis with SUB1 locus enhances submergence tolerance in rice
title qSUB2: A novel QTL with positive epistasis with SUB1 locus enhances submergence tolerance in rice
title_full qSUB2: A novel QTL with positive epistasis with SUB1 locus enhances submergence tolerance in rice
title_fullStr qSUB2: A novel QTL with positive epistasis with SUB1 locus enhances submergence tolerance in rice
title_full_unstemmed qSUB2: A novel QTL with positive epistasis with SUB1 locus enhances submergence tolerance in rice
title_short qSUB2: A novel QTL with positive epistasis with SUB1 locus enhances submergence tolerance in rice
title_sort qsub2 a novel qtl with positive epistasis with sub1 locus enhances submergence tolerance in rice
topic genotyping
tolerance
rice
url https://hdl.handle.net/10568/132705
work_keys_str_mv AT akintayoo qsub2anovelqtlwithpositiveepistasiswithsub1locusenhancessubmergencetoleranceinrice
AT danieli qsub2anovelqtlwithpositiveepistasiswithsub1locusenhancessubmergencetoleranceinrice
AT afeezs qsub2anovelqtlwithpositiveepistasiswithsub1locusenhancessubmergencetoleranceinrice
AT jolayemio qsub2anovelqtlwithpositiveepistasiswithsub1locusenhancessubmergencetoleranceinrice
AT semwalv qsub2anovelqtlwithpositiveepistasiswithsub1locusenhancessubmergencetoleranceinrice
AT venuprasadr qsub2anovelqtlwithpositiveepistasiswithsub1locusenhancessubmergencetoleranceinrice