Genetic variation among elite inbred lines suggests potential to breed for BNI-capacity in maize
Biological nitrification inhibition (BNI) is a plant function where root systems release antibiotic compounds (BNIs) specifically aimed at suppressing nitrifiers to limit soil-nitrate formation in the root zone. Little is known about BNI-activity in maize (Zea mays L.), the most important food, feed...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Nature Publishing Group
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/132645 |
| _version_ | 1855522200464916480 |
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| author | Petroli, César D. Guntur Venkata Subbarao Burgueño, Juan Tadashi Yoshihashi Huihui Li Franco, Jorge Pixley, Kevin V. |
| author_browse | Burgueño, Juan Franco, Jorge Guntur Venkata Subbarao Huihui Li Petroli, César D. Pixley, Kevin V. Tadashi Yoshihashi |
| author_facet | Petroli, César D. Guntur Venkata Subbarao Burgueño, Juan Tadashi Yoshihashi Huihui Li Franco, Jorge Pixley, Kevin V. |
| author_sort | Petroli, César D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Biological nitrification inhibition (BNI) is a plant function where root systems release antibiotic compounds (BNIs) specifically aimed at suppressing nitrifiers to limit soil-nitrate formation in the root zone. Little is known about BNI-activity in maize (Zea mays L.), the most important food, feed, and energy crop. Two categories of BNIs are released from maize roots; hydrophobic and hydrophilic BNIs, that determine BNI-capacity in root systems. Zeanone is a recently discovered hydrophobic compound with BNI-activity, released from maize roots. The objectives of this study were to understand/quantify the relationship between zeanone activity and hydrophobic BNI-capacity. We assessed genetic variability among 250 CIMMYT maize lines (CMLs) characterized for hydrophobic BNI-capacity and zeanone activity, towards developing genetic markers linked to this trait in maize. CMLs with high BNI-capacity and ability to release zeanone from roots were identified. GWAS was performed using 27,085 SNPs (with unique positions on the B73v.4 reference genome, and false discovery rate = 10), and phenotypic information for BNI-capacity and zeanone production from root systems. Eighteen significant markers were identified; three associated with specific BNI-activity (SBNI), four with BNI-activity per plant (BNIPP), another ten were common between SBNI and BNIPP, and one with zeanone release. Further, 30 annotated genes were associated with the significant SNPs; most of these genes are involved in pathways of "biological process", and one (AMT5) in ammonium regulation in maize roots. Although the inbred lines in this study were not developed for BNI-traits, the identification of markers associated with BNI-capacity suggests the possibility of using these genomic tools in marker-assisted selection to improve hydrophobic BNI-capacity in maize. |
| format | Journal Article |
| id | CGSpace132645 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Nature Publishing Group |
| publisherStr | Nature Publishing Group |
| record_format | dspace |
| spelling | CGSpace1326452025-11-06T13:04:52Z Genetic variation among elite inbred lines suggests potential to breed for BNI-capacity in maize Petroli, César D. Guntur Venkata Subbarao Burgueño, Juan Tadashi Yoshihashi Huihui Li Franco, Jorge Pixley, Kevin V. genetic variation inbred lines maize marker-assisted selection Biological nitrification inhibition (BNI) is a plant function where root systems release antibiotic compounds (BNIs) specifically aimed at suppressing nitrifiers to limit soil-nitrate formation in the root zone. Little is known about BNI-activity in maize (Zea mays L.), the most important food, feed, and energy crop. Two categories of BNIs are released from maize roots; hydrophobic and hydrophilic BNIs, that determine BNI-capacity in root systems. Zeanone is a recently discovered hydrophobic compound with BNI-activity, released from maize roots. The objectives of this study were to understand/quantify the relationship between zeanone activity and hydrophobic BNI-capacity. We assessed genetic variability among 250 CIMMYT maize lines (CMLs) characterized for hydrophobic BNI-capacity and zeanone activity, towards developing genetic markers linked to this trait in maize. CMLs with high BNI-capacity and ability to release zeanone from roots were identified. GWAS was performed using 27,085 SNPs (with unique positions on the B73v.4 reference genome, and false discovery rate = 10), and phenotypic information for BNI-capacity and zeanone production from root systems. Eighteen significant markers were identified; three associated with specific BNI-activity (SBNI), four with BNI-activity per plant (BNIPP), another ten were common between SBNI and BNIPP, and one with zeanone release. Further, 30 annotated genes were associated with the significant SNPs; most of these genes are involved in pathways of "biological process", and one (AMT5) in ammonium regulation in maize roots. Although the inbred lines in this study were not developed for BNI-traits, the identification of markers associated with BNI-capacity suggests the possibility of using these genomic tools in marker-assisted selection to improve hydrophobic BNI-capacity in maize. 2023 2023-11-01T16:59:39Z 2023-11-01T16:59:39Z Journal Article https://hdl.handle.net/10568/132645 en Open Access application/pdf Nature Publishing Group Petroli, C. D., Subbarao, G. V., Burgueño, J. A., Yoshihashi, T., Li, H., Franco Duran, J., & Pixley, K. V. (2023). Genetic variation among elite inbred lines suggests potential to breed for BNI-capacity in maize. Scientific Reports, 13(1). https://doi.org/10.1038/s41598-023-39720-3 |
| spellingShingle | genetic variation inbred lines maize marker-assisted selection Petroli, César D. Guntur Venkata Subbarao Burgueño, Juan Tadashi Yoshihashi Huihui Li Franco, Jorge Pixley, Kevin V. Genetic variation among elite inbred lines suggests potential to breed for BNI-capacity in maize |
| title | Genetic variation among elite inbred lines suggests potential to breed for BNI-capacity in maize |
| title_full | Genetic variation among elite inbred lines suggests potential to breed for BNI-capacity in maize |
| title_fullStr | Genetic variation among elite inbred lines suggests potential to breed for BNI-capacity in maize |
| title_full_unstemmed | Genetic variation among elite inbred lines suggests potential to breed for BNI-capacity in maize |
| title_short | Genetic variation among elite inbred lines suggests potential to breed for BNI-capacity in maize |
| title_sort | genetic variation among elite inbred lines suggests potential to breed for bni capacity in maize |
| topic | genetic variation inbred lines maize marker-assisted selection |
| url | https://hdl.handle.net/10568/132645 |
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