Convergently selected NPF2.12 coordinates root growth andnitrogen use efficiency in wheat and barley
- Understanding the genetic and molecular function of nitrate sensing and acquisition across crop species will accelerate breeding of cultivars with improved nitrogen use efficiency (NUE). - Here, we performed a genome-wide scan using wheat and barley accessions characterized under low and high N in...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2023
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/132632 |
| _version_ | 1855533035878875136 |
|---|---|
| author | Siddiqui, Nurealam Pandey, Kailash Kumer Bhadhury, Suzan Sadeqi, Bahman Schneider, Michael Sanchez-Garcia, Miguel Stich, Benjamin Schaaf, Gabriel Leon, Jens Ballvora, Agim |
| author_browse | Ballvora, Agim Kumer Bhadhury, Suzan Leon, Jens Pandey, Kailash Sadeqi, Bahman Sanchez-Garcia, Miguel Schaaf, Gabriel Schneider, Michael Siddiqui, Nurealam Stich, Benjamin |
| author_facet | Siddiqui, Nurealam Pandey, Kailash Kumer Bhadhury, Suzan Sadeqi, Bahman Schneider, Michael Sanchez-Garcia, Miguel Stich, Benjamin Schaaf, Gabriel Leon, Jens Ballvora, Agim |
| author_sort | Siddiqui, Nurealam |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | - Understanding the genetic and molecular function of nitrate sensing and acquisition across crop species will accelerate breeding of cultivars with improved nitrogen use efficiency (NUE).
- Here, we performed a genome-wide scan using wheat and barley accessions characterized under low and high N inputs that uncovered the NPF2.12 gene, encoding a homolog of the Arabidopsis nitrate transceptor NRT1.6 and other low-affinity nitrate transporters that belong to the MAJOR FACILITATOR SUPERFAMILY.
- Next, it is shown that variations in the NPF2.12 promoter correlated with altered NPF2.12 transcript levels where decreased gene expression was measured under low nitrate availability. Multiple field trials revealed a significantly enhanced N content in leaves and grains and NUE in the presence of the elite allele TaNPF2.12TT grown under low N conditions. Furthermore, the nitrate reductase encoding gene NIA1 was up-regulated in npf2.12 mutant upon low nitrate concentrations, thereby resulting in elevated levels of nitric oxide (NO) production. This increase in NO correlated with the higher root growth, nitrate uptake, and N translocation observed in the mutant when compared to wild-type.
- The presented data indicate that the elite haplotype alleles of NPF2.12 are convergently selected in wheat and barley that by inactivation indirectly contribute to root growth and NUE by activating NO signaling under low nitrate conditions. |
| format | Journal Article |
| id | CGSpace132632 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1326322026-01-15T02:13:29Z Convergently selected NPF2.12 coordinates root growth andnitrogen use efficiency in wheat and barley Siddiqui, Nurealam Pandey, Kailash Kumer Bhadhury, Suzan Sadeqi, Bahman Schneider, Michael Sanchez-Garcia, Miguel Stich, Benjamin Schaaf, Gabriel Leon, Jens Ballvora, Agim genetic variation cereals nitrogen use efficiency root system architecture barley wheat genome-wide association mapping nitrate transport - Understanding the genetic and molecular function of nitrate sensing and acquisition across crop species will accelerate breeding of cultivars with improved nitrogen use efficiency (NUE). - Here, we performed a genome-wide scan using wheat and barley accessions characterized under low and high N inputs that uncovered the NPF2.12 gene, encoding a homolog of the Arabidopsis nitrate transceptor NRT1.6 and other low-affinity nitrate transporters that belong to the MAJOR FACILITATOR SUPERFAMILY. - Next, it is shown that variations in the NPF2.12 promoter correlated with altered NPF2.12 transcript levels where decreased gene expression was measured under low nitrate availability. Multiple field trials revealed a significantly enhanced N content in leaves and grains and NUE in the presence of the elite allele TaNPF2.12TT grown under low N conditions. Furthermore, the nitrate reductase encoding gene NIA1 was up-regulated in npf2.12 mutant upon low nitrate concentrations, thereby resulting in elevated levels of nitric oxide (NO) production. This increase in NO correlated with the higher root growth, nitrate uptake, and N translocation observed in the mutant when compared to wild-type. - The presented data indicate that the elite haplotype alleles of NPF2.12 are convergently selected in wheat and barley that by inactivation indirectly contribute to root growth and NUE by activating NO signaling under low nitrate conditions. 2023-06-01 2023-11-01T14:45:43Z 2023-11-01T14:45:43Z Journal Article https://hdl.handle.net/10568/132632 en Open Access application/pdf Wiley Nurealam Siddiqui, Kailash Pandey, Suzan Kumer Bhadhury, Bahman Sadeqi, Michael Schneider, Miguel Sanchez-Garcia, Benjamin Stich, Gabriel Schaaf, Jens Leon, Agim Ballvora. (1/6/2023). Convergently selected NPF2. 12 coordinates root growth andnitrogen use efficiency in wheat and barley. New Phytologist, 238 (5), pp. 2175-2193. |
| spellingShingle | genetic variation cereals nitrogen use efficiency root system architecture barley wheat genome-wide association mapping nitrate transport Siddiqui, Nurealam Pandey, Kailash Kumer Bhadhury, Suzan Sadeqi, Bahman Schneider, Michael Sanchez-Garcia, Miguel Stich, Benjamin Schaaf, Gabriel Leon, Jens Ballvora, Agim Convergently selected NPF2.12 coordinates root growth andnitrogen use efficiency in wheat and barley |
| title | Convergently selected NPF2.12 coordinates root growth andnitrogen use efficiency in wheat and barley |
| title_full | Convergently selected NPF2.12 coordinates root growth andnitrogen use efficiency in wheat and barley |
| title_fullStr | Convergently selected NPF2.12 coordinates root growth andnitrogen use efficiency in wheat and barley |
| title_full_unstemmed | Convergently selected NPF2.12 coordinates root growth andnitrogen use efficiency in wheat and barley |
| title_short | Convergently selected NPF2.12 coordinates root growth andnitrogen use efficiency in wheat and barley |
| title_sort | convergently selected npf2 12 coordinates root growth andnitrogen use efficiency in wheat and barley |
| topic | genetic variation cereals nitrogen use efficiency root system architecture barley wheat genome-wide association mapping nitrate transport |
| url | https://hdl.handle.net/10568/132632 |
| work_keys_str_mv | AT siddiquinurealam convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley AT pandeykailash convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley AT kumerbhadhurysuzan convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley AT sadeqibahman convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley AT schneidermichael convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley AT sanchezgarciamiguel convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley AT stichbenjamin convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley AT schaafgabriel convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley AT leonjens convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley AT ballvoraagim convergentlyselectednpf212coordinatesrootgrowthandnitrogenuseefficiencyinwheatandbarley |