DNA sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests
Arbuscular mycorrhizal fungi (AMF) play a key role in medicinal plant species, besides their ecological role in shaping plant communities. Knowledge about the diversity and structure of AMF communities associated with the endangered Prunus africana is valuable in the conservation and domestication o...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2023
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| Online Access: | https://hdl.handle.net/10568/132572 |
| _version_ | 1855537859412361216 |
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| author | Tchiechoua, Yves H. Odee, David W. Ngonkeu, Eddy L.M. Kinyua, Johnson Ngumi, Victoria W. Machuka, Eunice M. Waweru, Bernice Le Roux, Marcellous Pelle, Roger |
| author_browse | Kinyua, Johnson Le Roux, Marcellous Machuka, Eunice M. Ngonkeu, Eddy L.M. Ngumi, Victoria W. Odee, David W. Pelle, Roger Tchiechoua, Yves H. Waweru, Bernice |
| author_facet | Tchiechoua, Yves H. Odee, David W. Ngonkeu, Eddy L.M. Kinyua, Johnson Ngumi, Victoria W. Machuka, Eunice M. Waweru, Bernice Le Roux, Marcellous Pelle, Roger |
| author_sort | Tchiechoua, Yves H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Arbuscular mycorrhizal fungi (AMF) play a key role in medicinal plant species, besides their ecological role in shaping plant communities. Knowledge about the diversity and structure of AMF communities associated with the endangered Prunus africana is valuable in the conservation and domestication of the species for its medicinal products.We investigated the diversity and structure of AMF species communities in the rhizosphere soils of P. africana trees occurring in four fragmented Afromontane forests found in Cameroon (Mount Cameroon and Mount Manengouba) and Kenya (Chuka and Malava) using Illumina Miseq sequencing of 18S rRNA gene amplicons.A total of 64 virtual taxa (VT) belonging to eight genera were detected, namely Glomus (43 VT), Claroideoglomus (6 VT), Paraglomus (5 VT), Acaulospora (4 VT), Diversispora (3 VT), and Archaeospora, Pacispora, and Scutellospora with 1 VT each. Scutellospora heterogama VTX00286 was the most abundant and common species in all four sites (49.62%). Glomeraceae and Gigasporaceae were the most abundant families found across the sites, while Acaulosporaceae, Pacisporaceae, and Archaeosporaceae were rare, represented by < 1% of all the detected taxa.Our data shows a high diversity of AMF species associated with P. africana and variable community structure partially shaped by local edaphic factors. |
| format | Journal Article |
| id | CGSpace132572 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1325722024-08-27T10:37:02Z DNA sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests Tchiechoua, Yves H. Odee, David W. Ngonkeu, Eddy L.M. Kinyua, Johnson Ngumi, Victoria W. Machuka, Eunice M. Waweru, Bernice Le Roux, Marcellous Pelle, Roger biotechnology communities dna domestication forests fungi soil species trees Arbuscular mycorrhizal fungi (AMF) play a key role in medicinal plant species, besides their ecological role in shaping plant communities. Knowledge about the diversity and structure of AMF communities associated with the endangered Prunus africana is valuable in the conservation and domestication of the species for its medicinal products.We investigated the diversity and structure of AMF species communities in the rhizosphere soils of P. africana trees occurring in four fragmented Afromontane forests found in Cameroon (Mount Cameroon and Mount Manengouba) and Kenya (Chuka and Malava) using Illumina Miseq sequencing of 18S rRNA gene amplicons.A total of 64 virtual taxa (VT) belonging to eight genera were detected, namely Glomus (43 VT), Claroideoglomus (6 VT), Paraglomus (5 VT), Acaulospora (4 VT), Diversispora (3 VT), and Archaeospora, Pacispora, and Scutellospora with 1 VT each. Scutellospora heterogama VTX00286 was the most abundant and common species in all four sites (49.62%). Glomeraceae and Gigasporaceae were the most abundant families found across the sites, while Acaulosporaceae, Pacisporaceae, and Archaeosporaceae were rare, represented by < 1% of all the detected taxa.Our data shows a high diversity of AMF species associated with P. africana and variable community structure partially shaped by local edaphic factors. 2023-05-25 2023-10-31T12:27:30Z 2023-10-31T12:27:30Z Journal Article https://hdl.handle.net/10568/132572 en Open Access Springer Tchiechoua, Yves H. et al. 2023. DNA sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests. Annals of Microbiology 73(1) |
| spellingShingle | biotechnology communities dna domestication forests fungi soil species trees Tchiechoua, Yves H. Odee, David W. Ngonkeu, Eddy L.M. Kinyua, Johnson Ngumi, Victoria W. Machuka, Eunice M. Waweru, Bernice Le Roux, Marcellous Pelle, Roger DNA sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests |
| title | DNA sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests |
| title_full | DNA sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests |
| title_fullStr | DNA sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests |
| title_full_unstemmed | DNA sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests |
| title_short | DNA sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of Prunus africana trees in fragmented Afromontane forests |
| title_sort | dna sequencing reveals high arbuscular mycorrhizal fungi diversity in the rhizosphere soil of prunus africana trees in fragmented afromontane forests |
| topic | biotechnology communities dna domestication forests fungi soil species trees |
| url | https://hdl.handle.net/10568/132572 |
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