Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains
Micronutrient deficiencies, particularly of iron (Fe) and zinc (Zn), in the diet contribute to health issues and hidden hunger. Enhancing the Fe and Zn content in globally staple food crops like rice is necessary to address food malnutrition. A Genome-Wide Association Study (GWAS) was conducted usin...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/132533 |
| _version_ | 1855535381655584768 |
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| author | Bukomarhe, C.B. Kimwemwe, Paul Kitenge Githiri, S.M. Mamati, Edward George Kimani, Wilson Mutai, Collins Ng'ang'a, Fredrick M. Nguezet, P.-M.D. Mignouna, J. Civava, R.M. Fofana, M. |
| author_browse | Bukomarhe, C.B. Civava, R.M. Fofana, M. Githiri, S.M. Kimani, Wilson Kimwemwe, Paul Kitenge Mamati, Edward George Mignouna, J. Mutai, Collins Ng'ang'a, Fredrick M. Nguezet, P.-M.D. |
| author_facet | Bukomarhe, C.B. Kimwemwe, Paul Kitenge Githiri, S.M. Mamati, Edward George Kimani, Wilson Mutai, Collins Ng'ang'a, Fredrick M. Nguezet, P.-M.D. Mignouna, J. Civava, R.M. Fofana, M. |
| author_sort | Bukomarhe, C.B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Micronutrient deficiencies, particularly of iron (Fe) and zinc (Zn), in the diet contribute to health issues and hidden hunger. Enhancing the Fe and Zn content in globally staple food crops like rice is necessary to address food malnutrition. A Genome-Wide Association Study (GWAS) was conducted using 85 diverse rice accessions from the Democratic Republic of Congo (DRC) to identify genomic regions associated with grain Fe and Zn content. The Fe content ranged from 0.95 to 8.68 mg/100 g on a dry weight basis (dwb) while Zn content ranged from 0.87 to 3.8 mg/100 g (dwb). Using MLM and FarmCPU models, we found 10 significant SNPs out of which one SNP on chromosome 11 was associated with the variation in Fe content and one SNP on chromosome 4 was associated with the Zn content, and both were commonly detected by the two models. Candidate genes belonging to transcription regulator activities, including the bZIP family genes and MYB family genes, as well as transporter activities involved in Fe and Zn homeostasis were identified in the vicinity of the SNP markers and selected. The identified SNP markers hold promise for marker-assisted selection in rice breeding programs aimed at enhancing Fe and Zn content in rice. This study provides valuable insights into the genetic factors controlling Fe and Zn uptake and their transport and accumulation in rice, offering opportunities for developing biofortified rice varieties to combat malnutrition among rice consumers. |
| format | Journal Article |
| id | CGSpace132533 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1325332025-12-08T10:29:22Z Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains Bukomarhe, C.B. Kimwemwe, Paul Kitenge Githiri, S.M. Mamati, Edward George Kimani, Wilson Mutai, Collins Ng'ang'a, Fredrick M. Nguezet, P.-M.D. Mignouna, J. Civava, R.M. Fofana, M. rice zinc oryza sativa plant genetics Micronutrient deficiencies, particularly of iron (Fe) and zinc (Zn), in the diet contribute to health issues and hidden hunger. Enhancing the Fe and Zn content in globally staple food crops like rice is necessary to address food malnutrition. A Genome-Wide Association Study (GWAS) was conducted using 85 diverse rice accessions from the Democratic Republic of Congo (DRC) to identify genomic regions associated with grain Fe and Zn content. The Fe content ranged from 0.95 to 8.68 mg/100 g on a dry weight basis (dwb) while Zn content ranged from 0.87 to 3.8 mg/100 g (dwb). Using MLM and FarmCPU models, we found 10 significant SNPs out of which one SNP on chromosome 11 was associated with the variation in Fe content and one SNP on chromosome 4 was associated with the Zn content, and both were commonly detected by the two models. Candidate genes belonging to transcription regulator activities, including the bZIP family genes and MYB family genes, as well as transporter activities involved in Fe and Zn homeostasis were identified in the vicinity of the SNP markers and selected. The identified SNP markers hold promise for marker-assisted selection in rice breeding programs aimed at enhancing Fe and Zn content in rice. This study provides valuable insights into the genetic factors controlling Fe and Zn uptake and their transport and accumulation in rice, offering opportunities for developing biofortified rice varieties to combat malnutrition among rice consumers. 2023-09-18 2023-10-30T15:11:30Z 2023-10-30T15:11:30Z Journal Article https://hdl.handle.net/10568/132533 en Open Access MDPI Bukomarhe, C.B., Kimwemwe, P.K., Githiri, S.M., Mamati, E.G., Kimani, W., Mutai, C., Nganga, F., Nguezet, P.-M.D., Mignouna, J., Civava, R.M. and Fofana, M. 2023. Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains. Genes 14:1815. |
| spellingShingle | rice zinc oryza sativa plant genetics Bukomarhe, C.B. Kimwemwe, Paul Kitenge Githiri, S.M. Mamati, Edward George Kimani, Wilson Mutai, Collins Ng'ang'a, Fredrick M. Nguezet, P.-M.D. Mignouna, J. Civava, R.M. Fofana, M. Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains |
| title | Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains |
| title_full | Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains |
| title_fullStr | Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains |
| title_full_unstemmed | Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains |
| title_short | Association mapping of candidate genes associated with iron and zinc content in rice (Oryza sativa L.) grains |
| title_sort | association mapping of candidate genes associated with iron and zinc content in rice oryza sativa l grains |
| topic | rice zinc oryza sativa plant genetics |
| url | https://hdl.handle.net/10568/132533 |
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