Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group
Characterization of major resistance (R) genes to late blight (LB) –caused by the oomycete Phytophthora infestans– is very important for potato breeding. The objective of this study was to identify novel genes for resistance to LB from diploid Solanum tuberosum L. Andigenum Group (StAG) cultivar...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Frontiers Media
2023
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/132274 |
| _version_ | 1855538480520626176 |
|---|---|
| author | Cruz, G. de la Blas, R. Pérez, W. Neyra, E. Ortíz, R. |
| author_browse | Blas, R. Cruz, G. de la Neyra, E. Ortíz, R. Pérez, W. |
| author_facet | Cruz, G. de la Blas, R. Pérez, W. Neyra, E. Ortíz, R. |
| author_sort | Cruz, G. de la |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Characterization of major resistance (R) genes to late blight (LB) –caused by the
oomycete Phytophthora infestans– is very important for potato breeding. The
objective of this study was to identify novel genes for resistance to LB from
diploid Solanum tuberosum L. Andigenum Group (StAG) cultivar accessions.
Using comparative analysis with a edgeR bioconductor package for differential
expression analysis of transcriptomes, two of these accessions with contrasting
levels of resistance to LB were analyzed using digital gene expression data. As a
result, various differentially expressed genes (P ≤ 0.0001, Log2FC ≥ 2, FDR <
0.001) were noted. The combination of transcriptomic analysis provided 303
candidate genes that are overexpressed and underexpressed, thereby giving high
resistance to LB. The functional analysis showed differential expression of R
genes and their corresponding proteins related to disease resistance, NBS-LRR
domain proteins, and specific disease resistance proteins. Comparative analysis
of specific tissue transcriptomes in resistant and susceptible genotypes can be
used for rapidly identifying candidate R genes, thus adding novel genes from
diploid StAG cultivar accessions for host plant resistance to P. infestans in potato. |
| format | Journal Article |
| id | CGSpace132274 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1322742025-12-08T10:29:22Z Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group Cruz, G. de la Blas, R. Pérez, W. Neyra, E. Ortíz, R. gene expression genomes host plant resistance phytophthora infestans solanum tuberosum Characterization of major resistance (R) genes to late blight (LB) –caused by the oomycete Phytophthora infestans– is very important for potato breeding. The objective of this study was to identify novel genes for resistance to LB from diploid Solanum tuberosum L. Andigenum Group (StAG) cultivar accessions. Using comparative analysis with a edgeR bioconductor package for differential expression analysis of transcriptomes, two of these accessions with contrasting levels of resistance to LB were analyzed using digital gene expression data. As a result, various differentially expressed genes (P ≤ 0.0001, Log2FC ≥ 2, FDR < 0.001) were noted. The combination of transcriptomic analysis provided 303 candidate genes that are overexpressed and underexpressed, thereby giving high resistance to LB. The functional analysis showed differential expression of R genes and their corresponding proteins related to disease resistance, NBS-LRR domain proteins, and specific disease resistance proteins. Comparative analysis of specific tissue transcriptomes in resistant and susceptible genotypes can be used for rapidly identifying candidate R genes, thus adding novel genes from diploid StAG cultivar accessions for host plant resistance to P. infestans in potato. 2023-09-11 2023-10-16T22:13:03Z 2023-10-16T22:13:03Z Journal Article https://hdl.handle.net/10568/132274 en Open Access Frontiers Media De La Cruz, G.; Blas, R.; Pérez, W.; Neyra, E.; Ortíz, R. 2023. Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group. Frontiers in Plant Science, 14. ISSN 1664-462X. |
| spellingShingle | gene expression genomes host plant resistance phytophthora infestans solanum tuberosum Cruz, G. de la Blas, R. Pérez, W. Neyra, E. Ortíz, R. Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group |
| title | Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group |
| title_full | Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group |
| title_fullStr | Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group |
| title_full_unstemmed | Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group |
| title_short | Foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato Solanum tuberosum L. Andigenum Group |
| title_sort | foliar transcriptomes reveal candidate genes for late blight resistance in cultivars of diploid potato solanum tuberosum l andigenum group |
| topic | gene expression genomes host plant resistance phytophthora infestans solanum tuberosum |
| url | https://hdl.handle.net/10568/132274 |
| work_keys_str_mv | AT cruzgdela foliartranscriptomesrevealcandidategenesforlateblightresistanceincultivarsofdiploidpotatosolanumtuberosumlandigenumgroup AT blasr foliartranscriptomesrevealcandidategenesforlateblightresistanceincultivarsofdiploidpotatosolanumtuberosumlandigenumgroup AT perezw foliartranscriptomesrevealcandidategenesforlateblightresistanceincultivarsofdiploidpotatosolanumtuberosumlandigenumgroup AT neyrae foliartranscriptomesrevealcandidategenesforlateblightresistanceincultivarsofdiploidpotatosolanumtuberosumlandigenumgroup AT ortizr foliartranscriptomesrevealcandidategenesforlateblightresistanceincultivarsofdiploidpotatosolanumtuberosumlandigenumgroup |