Multiple year influences of the aflatoxin biocontrol product AF-X1 on the A. flavus communities associated with maize production in Italy

AF-X1 is a commercial aflatoxin biocontrol product containing the non-aflatoxigenic (AF-) strain of Aspergillus flavus MUCL54911 (VCG IT006), endemic to Italy, as an active ingredient. The present study aimed to evaluate the long-term persistence of VCG IT006 in the treated fields, and the multi-yea...

Descripción completa

Detalles Bibliográficos
Autores principales: Ouadhene, M.A., Ortega-Beltran, A., Sanna, M., Cotty, P.J., Battilani, P.
Formato: Journal Article
Lenguaje:Inglés
Publicado: MDPI 2023
Materias:
Acceso en línea:https://hdl.handle.net/10568/132270
_version_ 1855519996596191232
author Ouadhene, M.A.
Ortega-Beltran, A.
Sanna, M.
Cotty, P.J.
Battilani, P.
author_browse Battilani, P.
Cotty, P.J.
Ortega-Beltran, A.
Ouadhene, M.A.
Sanna, M.
author_facet Ouadhene, M.A.
Ortega-Beltran, A.
Sanna, M.
Cotty, P.J.
Battilani, P.
author_sort Ouadhene, M.A.
collection Repository of Agricultural Research Outputs (CGSpace)
description AF-X1 is a commercial aflatoxin biocontrol product containing the non-aflatoxigenic (AF-) strain of Aspergillus flavus MUCL54911 (VCG IT006), endemic to Italy, as an active ingredient. The present study aimed to evaluate the long-term persistence of VCG IT006 in the treated fields, and the multi-year influence of the biocontrol application on the A. flavus population. Soil samples were collected in 2020 and 2021 from 28 fields located in four provinces in north Italy. A vegetative compatibility analysis was conducted to monitor the occurrence of VCG IT006 on the total of the 399 isolates of A. flavus that were collected. IT006 was present in all the fields, mainly in the fields treated for 1 yr or 2 consecutive yrs (58% and 63%, respectively). The densities of the toxigenic isolates, detected using the aflR gene, were 45% vs. 22% in the untreated and treated fields, respectively. After displacement via the AF- deployment, a variability from 7% to 32% was noticed in the toxigenic isolates. The current findings support the long-term durability of the biocontrol application benefits without deleterious effects on each fungal population. Nevertheless, based on the current results, as well as on previous studies, the yearly applications of AF-X1 to Italian commercial maize fields should continue.
format Journal Article
id CGSpace132270
institution CGIAR Consortium
language Inglés
publishDate 2023
publishDateRange 2023
publishDateSort 2023
publisher MDPI
publisherStr MDPI
record_format dspace
spelling CGSpace1322702025-12-08T10:29:22Z Multiple year influences of the aflatoxin biocontrol product AF-X1 on the A. flavus communities associated with maize production in Italy Ouadhene, M.A. Ortega-Beltran, A. Sanna, M. Cotty, P.J. Battilani, P. maize food security aflatoxins biological control aspergillus flavus AF-X1 is a commercial aflatoxin biocontrol product containing the non-aflatoxigenic (AF-) strain of Aspergillus flavus MUCL54911 (VCG IT006), endemic to Italy, as an active ingredient. The present study aimed to evaluate the long-term persistence of VCG IT006 in the treated fields, and the multi-year influence of the biocontrol application on the A. flavus population. Soil samples were collected in 2020 and 2021 from 28 fields located in four provinces in north Italy. A vegetative compatibility analysis was conducted to monitor the occurrence of VCG IT006 on the total of the 399 isolates of A. flavus that were collected. IT006 was present in all the fields, mainly in the fields treated for 1 yr or 2 consecutive yrs (58% and 63%, respectively). The densities of the toxigenic isolates, detected using the aflR gene, were 45% vs. 22% in the untreated and treated fields, respectively. After displacement via the AF- deployment, a variability from 7% to 32% was noticed in the toxigenic isolates. The current findings support the long-term durability of the biocontrol application benefits without deleterious effects on each fungal population. Nevertheless, based on the current results, as well as on previous studies, the yearly applications of AF-X1 to Italian commercial maize fields should continue. 2023 2023-10-16T10:56:26Z 2023-10-16T10:56:26Z Journal Article https://hdl.handle.net/10568/132270 en Open Access application/pdf MDPI Ouadhene, M.A., Ortega-Beltran, A., Sanna, M., Cotty, P.J. & Battilani, P. (2023). Multiple year influences of the aflatoxin biocontrol product AF-X1 on the A. flavus communities associated with maize production in Italy. Toxins, 15(3): 184, 1-15.
spellingShingle maize
food security
aflatoxins
biological control
aspergillus flavus
Ouadhene, M.A.
Ortega-Beltran, A.
Sanna, M.
Cotty, P.J.
Battilani, P.
Multiple year influences of the aflatoxin biocontrol product AF-X1 on the A. flavus communities associated with maize production in Italy
title Multiple year influences of the aflatoxin biocontrol product AF-X1 on the A. flavus communities associated with maize production in Italy
title_full Multiple year influences of the aflatoxin biocontrol product AF-X1 on the A. flavus communities associated with maize production in Italy
title_fullStr Multiple year influences of the aflatoxin biocontrol product AF-X1 on the A. flavus communities associated with maize production in Italy
title_full_unstemmed Multiple year influences of the aflatoxin biocontrol product AF-X1 on the A. flavus communities associated with maize production in Italy
title_short Multiple year influences of the aflatoxin biocontrol product AF-X1 on the A. flavus communities associated with maize production in Italy
title_sort multiple year influences of the aflatoxin biocontrol product af x1 on the a flavus communities associated with maize production in italy
topic maize
food security
aflatoxins
biological control
aspergillus flavus
url https://hdl.handle.net/10568/132270
work_keys_str_mv AT ouadhenema multipleyearinfluencesoftheaflatoxinbiocontrolproductafx1ontheaflavuscommunitiesassociatedwithmaizeproductioninitaly
AT ortegabeltrana multipleyearinfluencesoftheaflatoxinbiocontrolproductafx1ontheaflavuscommunitiesassociatedwithmaizeproductioninitaly
AT sannam multipleyearinfluencesoftheaflatoxinbiocontrolproductafx1ontheaflavuscommunitiesassociatedwithmaizeproductioninitaly
AT cottypj multipleyearinfluencesoftheaflatoxinbiocontrolproductafx1ontheaflavuscommunitiesassociatedwithmaizeproductioninitaly
AT battilanip multipleyearinfluencesoftheaflatoxinbiocontrolproductafx1ontheaflavuscommunitiesassociatedwithmaizeproductioninitaly