Aflatoxin contamination of maize and groundnut in Burundi: distribution of contamination, identification of causal agents and potential biocontrol genotypes of Aspergillus flavus
Aflatoxin contamination of the staples maize and groundnut is a concern for health and economic impacts across sub-Saharan Africa. The current study (i) determined aflatoxin levels in maize and groundnut collected at harvest in Burundi, (ii) characterized populations of Aspergillus section Flavi ass...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Frontiers Media
2023
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| Online Access: | https://hdl.handle.net/10568/132262 |
| _version_ | 1855518961940037632 |
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| author | Nsabiyumva, G. Mutegi, C. Wagacha, M. Mohamed, A.B. Njeru, N.K. Ndayihanzamaso, P. Niyuhire, C. Atehnkeng, J. Njukwe, E. Callicott, K. Cotty, P.J. Ortega-Beltran, A. Bandyopadhyay, R. |
| author_browse | Atehnkeng, J. Bandyopadhyay, R. Callicott, K. Cotty, P.J. Mohamed, A.B. Mutegi, C. Ndayihanzamaso, P. Niyuhire, C. Njeru, N.K. Njukwe, E. Nsabiyumva, G. Ortega-Beltran, A. Wagacha, M. |
| author_facet | Nsabiyumva, G. Mutegi, C. Wagacha, M. Mohamed, A.B. Njeru, N.K. Ndayihanzamaso, P. Niyuhire, C. Atehnkeng, J. Njukwe, E. Callicott, K. Cotty, P.J. Ortega-Beltran, A. Bandyopadhyay, R. |
| author_sort | Nsabiyumva, G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Aflatoxin contamination of the staples maize and groundnut is a concern for health and economic impacts across sub-Saharan Africa. The current study (i) determined aflatoxin levels in maize and groundnut collected at harvest in Burundi, (ii) characterized populations of Aspergillus section Flavi associated with the two crops, and (iii) assessed aflatoxin-producing potentials among the recovered fungi. A total of 120 groundnut and 380 maize samples were collected at harvest from eight and 16 provinces, respectively. Most of the groundnut (93%) and maize (87%) contained aflatoxin below the European Union threshold, 4 μg/kg. Morphological characterization of the recovered Aspergillus section Flavi fungi revealed that the L-morphotype of A. flavus was the predominant species. Aflatoxin production potentials of the L-morphotype isolates were evaluated in maize fermentations. Some isolates produced over 137,000 μg/kg aflatoxin B 1 . Thus, despite the relatively low aflatoxin levels at harvest, the association of both crops with highly toxigenic fungi poses significant risk of post-harvest aflatoxin contamination and suggests measures to mitigate aflatoxin contamination in Burundi should be developed. Over 55% of the L-morphotype A. flavus did not produce aflatoxins. These atoxigenic L-morphotype fungi were characterized using molecular markers. Several atoxigenic genotypes were detected across the country and could be used as biocontrol agents. The results from the current study hold promise for developing aflatoxin management strategies centered on biocontrol for use in Burundi to reduce aflatoxin contamination throughout the value chain. |
| format | Journal Article |
| id | CGSpace132262 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1322622025-12-08T10:29:22Z Aflatoxin contamination of maize and groundnut in Burundi: distribution of contamination, identification of causal agents and potential biocontrol genotypes of Aspergillus flavus Nsabiyumva, G. Mutegi, C. Wagacha, M. Mohamed, A.B. Njeru, N.K. Ndayihanzamaso, P. Niyuhire, C. Atehnkeng, J. Njukwe, E. Callicott, K. Cotty, P.J. Ortega-Beltran, A. Bandyopadhyay, R. maize food security biological control aspergillus flavus biodiversity value chain aflatoxins Aflatoxin contamination of the staples maize and groundnut is a concern for health and economic impacts across sub-Saharan Africa. The current study (i) determined aflatoxin levels in maize and groundnut collected at harvest in Burundi, (ii) characterized populations of Aspergillus section Flavi associated with the two crops, and (iii) assessed aflatoxin-producing potentials among the recovered fungi. A total of 120 groundnut and 380 maize samples were collected at harvest from eight and 16 provinces, respectively. Most of the groundnut (93%) and maize (87%) contained aflatoxin below the European Union threshold, 4 μg/kg. Morphological characterization of the recovered Aspergillus section Flavi fungi revealed that the L-morphotype of A. flavus was the predominant species. Aflatoxin production potentials of the L-morphotype isolates were evaluated in maize fermentations. Some isolates produced over 137,000 μg/kg aflatoxin B 1 . Thus, despite the relatively low aflatoxin levels at harvest, the association of both crops with highly toxigenic fungi poses significant risk of post-harvest aflatoxin contamination and suggests measures to mitigate aflatoxin contamination in Burundi should be developed. Over 55% of the L-morphotype A. flavus did not produce aflatoxins. These atoxigenic L-morphotype fungi were characterized using molecular markers. Several atoxigenic genotypes were detected across the country and could be used as biocontrol agents. The results from the current study hold promise for developing aflatoxin management strategies centered on biocontrol for use in Burundi to reduce aflatoxin contamination throughout the value chain. 2023 2023-10-15T16:55:46Z 2023-10-15T16:55:46Z Journal Article https://hdl.handle.net/10568/132262 en Open Access application/pdf Frontiers Media Nsabiyumva, G., Mutegi, C., Wagacha, M., Mohamed, A.B., Njeru, N.K., Ndayihanzamaso, P., ... & Bandyopadhyay, R. (2023). Aflatoxin contamination of maize and groundnut in Burundi: distribution of contamination, identification of causal agents and potential biocontrol genotypes of Aspergillus flavus. Frontiers in Microbiology, 14: 1106543, 1-13. |
| spellingShingle | maize food security biological control aspergillus flavus biodiversity value chain aflatoxins Nsabiyumva, G. Mutegi, C. Wagacha, M. Mohamed, A.B. Njeru, N.K. Ndayihanzamaso, P. Niyuhire, C. Atehnkeng, J. Njukwe, E. Callicott, K. Cotty, P.J. Ortega-Beltran, A. Bandyopadhyay, R. Aflatoxin contamination of maize and groundnut in Burundi: distribution of contamination, identification of causal agents and potential biocontrol genotypes of Aspergillus flavus |
| title | Aflatoxin contamination of maize and groundnut in Burundi: distribution of contamination, identification of causal agents and potential biocontrol genotypes of Aspergillus flavus |
| title_full | Aflatoxin contamination of maize and groundnut in Burundi: distribution of contamination, identification of causal agents and potential biocontrol genotypes of Aspergillus flavus |
| title_fullStr | Aflatoxin contamination of maize and groundnut in Burundi: distribution of contamination, identification of causal agents and potential biocontrol genotypes of Aspergillus flavus |
| title_full_unstemmed | Aflatoxin contamination of maize and groundnut in Burundi: distribution of contamination, identification of causal agents and potential biocontrol genotypes of Aspergillus flavus |
| title_short | Aflatoxin contamination of maize and groundnut in Burundi: distribution of contamination, identification of causal agents and potential biocontrol genotypes of Aspergillus flavus |
| title_sort | aflatoxin contamination of maize and groundnut in burundi distribution of contamination identification of causal agents and potential biocontrol genotypes of aspergillus flavus |
| topic | maize food security biological control aspergillus flavus biodiversity value chain aflatoxins |
| url | https://hdl.handle.net/10568/132262 |
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