High-density DArTSeq SNP markers revealed wide genetic diversity and structured population in common bean (Phaseolus vulgaris L.) germplasm in Ethiopia

Abstract Introduction Common bean is one of the widely consumed food security crop in Africa, Asia, and South America. Understanding genetic diversity and population structure is crucial for designing breeding strategies. Materials Two hundred and eighty-nine germplasm were recently collected from d...

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Autores principales: Gelaw, Yonas Moges, Eleblu, John S. Y., Ofori, Kwadwo, Fenta, Berhanu Amsalu, Mukankusi, Clare, Emam, Ermias Assefa, Offei, Samuel Kwame
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2023
Materias:
Acceso en línea:https://hdl.handle.net/10568/132159
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author Gelaw, Yonas Moges
Eleblu, John S. Y.
Ofori, Kwadwo
Fenta, Berhanu Amsalu
Mukankusi, Clare
Emam, Ermias Assefa
Offei, Samuel Kwame
author_browse Eleblu, John S. Y.
Emam, Ermias Assefa
Fenta, Berhanu Amsalu
Gelaw, Yonas Moges
Mukankusi, Clare
Offei, Samuel Kwame
Ofori, Kwadwo
author_facet Gelaw, Yonas Moges
Eleblu, John S. Y.
Ofori, Kwadwo
Fenta, Berhanu Amsalu
Mukankusi, Clare
Emam, Ermias Assefa
Offei, Samuel Kwame
author_sort Gelaw, Yonas Moges
collection Repository of Agricultural Research Outputs (CGSpace)
description Abstract Introduction Common bean is one of the widely consumed food security crop in Africa, Asia, and South America. Understanding genetic diversity and population structure is crucial for designing breeding strategies. Materials Two hundred and eighty-nine germplasm were recently collected from different regions of Ethiopia and introduced from CIAT to estimate genetic diversity and population structure using 11,480 DArTSeq SNP markers. Results The overall mean genetic diversity and polymorphic information content (PIC) were 0.38 and 0.30, respectively, suggested the presence of adequate genetic diversity among the genotypes. Among the geographical regions, landraces collected from Oromia showed the highest diversity (0.39) and PIC (0.30). The highest genetic distance was observed between genotypes collected from SNNPR and CIAT (0.49). In addition, genotypes from CIAT were genetically more related to improved varieties than the landraces which could be due to sharing of parents in the improvement process. The analysis of molecular variance revealed that the largest proportion of variation was due to within the population both in geographical region (63.67%) and breeding status (61.3%) based classification. Model-based structure analysis delineated the 289 common bean genotypes into six hypothetical ancestoral populations. Conclusions The genotypes were not clustered based on geographical regions and they were not the main drivers for the differentiation. This indicated that selection of the parental lines should be based on systematic assessment of the diversity rather than geographical distance. This article provides new insights into the genetic diversity and population structure of common bean for association studies, designing effective collection and conservation for efficient utilization for the improvement of the crop.
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spelling CGSpace1321592025-12-08T09:54:28Z High-density DArTSeq SNP markers revealed wide genetic diversity and structured population in common bean (Phaseolus vulgaris L.) germplasm in Ethiopia Gelaw, Yonas Moges Eleblu, John S. Y. Ofori, Kwadwo Fenta, Berhanu Amsalu Mukankusi, Clare Emam, Ermias Assefa Offei, Samuel Kwame genetic diversity (as resource) heterozygotes marker-assisted selection-marker assisted selection population structure resource conservation-conservation uses genotypes Abstract Introduction Common bean is one of the widely consumed food security crop in Africa, Asia, and South America. Understanding genetic diversity and population structure is crucial for designing breeding strategies. Materials Two hundred and eighty-nine germplasm were recently collected from different regions of Ethiopia and introduced from CIAT to estimate genetic diversity and population structure using 11,480 DArTSeq SNP markers. Results The overall mean genetic diversity and polymorphic information content (PIC) were 0.38 and 0.30, respectively, suggested the presence of adequate genetic diversity among the genotypes. Among the geographical regions, landraces collected from Oromia showed the highest diversity (0.39) and PIC (0.30). The highest genetic distance was observed between genotypes collected from SNNPR and CIAT (0.49). In addition, genotypes from CIAT were genetically more related to improved varieties than the landraces which could be due to sharing of parents in the improvement process. The analysis of molecular variance revealed that the largest proportion of variation was due to within the population both in geographical region (63.67%) and breeding status (61.3%) based classification. Model-based structure analysis delineated the 289 common bean genotypes into six hypothetical ancestoral populations. Conclusions The genotypes were not clustered based on geographical regions and they were not the main drivers for the differentiation. This indicated that selection of the parental lines should be based on systematic assessment of the diversity rather than geographical distance. This article provides new insights into the genetic diversity and population structure of common bean for association studies, designing effective collection and conservation for efficient utilization for the improvement of the crop. 2023-08 2023-10-06T11:18:45Z 2023-10-06T11:18:45Z Journal Article https://hdl.handle.net/10568/132159 en Open Access application/pdf Springer Gelaw, Y.M.; Eleblu, J.S.Y.; Ofori, K.; Fenta, B.A.; Mukankusi, C.; Emam, E.A.; Offei, S. (2023) High-density DArTSeq SNP markers revealed wide genetic diversity and structured population in common bean (Phaseolus vulgaris L.) germplasm in Ethiopia. Molecular Biology Reports 50(8): p. 6739-6751. ISSN: 0301-4851
spellingShingle genetic diversity (as resource)
heterozygotes
marker-assisted selection-marker assisted selection
population structure
resource conservation-conservation
uses
genotypes
Gelaw, Yonas Moges
Eleblu, John S. Y.
Ofori, Kwadwo
Fenta, Berhanu Amsalu
Mukankusi, Clare
Emam, Ermias Assefa
Offei, Samuel Kwame
High-density DArTSeq SNP markers revealed wide genetic diversity and structured population in common bean (Phaseolus vulgaris L.) germplasm in Ethiopia
title High-density DArTSeq SNP markers revealed wide genetic diversity and structured population in common bean (Phaseolus vulgaris L.) germplasm in Ethiopia
title_full High-density DArTSeq SNP markers revealed wide genetic diversity and structured population in common bean (Phaseolus vulgaris L.) germplasm in Ethiopia
title_fullStr High-density DArTSeq SNP markers revealed wide genetic diversity and structured population in common bean (Phaseolus vulgaris L.) germplasm in Ethiopia
title_full_unstemmed High-density DArTSeq SNP markers revealed wide genetic diversity and structured population in common bean (Phaseolus vulgaris L.) germplasm in Ethiopia
title_short High-density DArTSeq SNP markers revealed wide genetic diversity and structured population in common bean (Phaseolus vulgaris L.) germplasm in Ethiopia
title_sort high density dartseq snp markers revealed wide genetic diversity and structured population in common bean phaseolus vulgaris l germplasm in ethiopia
topic genetic diversity (as resource)
heterozygotes
marker-assisted selection-marker assisted selection
population structure
resource conservation-conservation
uses
genotypes
url https://hdl.handle.net/10568/132159
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