Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus
The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, Hoplolaimus seinhorsti and H. pararobustus, are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-bas...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Walter de Gruyter GmbH
2023
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/131990 |
| _version_ | 1855532156281946112 |
|---|---|
| author | Olajide, E. Singh, P.R. Kolombia, Y.A. Rumbarar, M.K. Couvreur, M. Bert, W. |
| author_browse | Bert, W. Couvreur, M. Kolombia, Y.A. Olajide, E. Rumbarar, M.K. Singh, P.R. |
| author_facet | Olajide, E. Singh, P.R. Kolombia, Y.A. Rumbarar, M.K. Couvreur, M. Bert, W. |
| author_sort | Olajide, E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, Hoplolaimus seinhorsti and H. pararobustus, are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-based molecular species delimitation methods (GMYC and bPTP). Nineteen new sequences were obtained, including 5 partial 18S rRNA, 6 D2-D3 of 28S rRNA, 1 ITS rRNA and 7 COI mtDNA (the first COI sequences of H. seinhorsti and H. pararobustus), and an updated morphological character comparison of 37 Hoplolaimus species is presented. The tree-based molecular species-delimitation approaches employed gave markedly differing results, and also showed remarkable discrepancies among the investigated genes, although the bPTP output was found to agree well with established morphological species delimitations. Both species-delimitation approaches did, however, provide the same output for the COI mtDNA sequences, and the COI mtDNA gene sequence was also found to correspond better to established morphological species. It is therefore recommended by this paper as representing the most suitable barcode marker for Hoplolaimus species identification. This integrative study also resulted in the corrective reassignment of 17 gene sequences that were previously unidentified or incorrectly classified, as well as concluding that H. pararobustus consists of two cryptic species. |
| format | Journal Article |
| id | CGSpace131990 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Walter de Gruyter GmbH |
| publisherStr | Walter de Gruyter GmbH |
| record_format | dspace |
| spelling | CGSpace1319902025-11-11T10:37:36Z Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus Olajide, E. Singh, P.R. Kolombia, Y.A. Rumbarar, M.K. Couvreur, M. Bert, W. bananas hoplolaimus nematodes morphology phylogeny plant nematodes taxonomy dna The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, Hoplolaimus seinhorsti and H. pararobustus, are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-based molecular species delimitation methods (GMYC and bPTP). Nineteen new sequences were obtained, including 5 partial 18S rRNA, 6 D2-D3 of 28S rRNA, 1 ITS rRNA and 7 COI mtDNA (the first COI sequences of H. seinhorsti and H. pararobustus), and an updated morphological character comparison of 37 Hoplolaimus species is presented. The tree-based molecular species-delimitation approaches employed gave markedly differing results, and also showed remarkable discrepancies among the investigated genes, although the bPTP output was found to agree well with established morphological species delimitations. Both species-delimitation approaches did, however, provide the same output for the COI mtDNA sequences, and the COI mtDNA gene sequence was also found to correspond better to established morphological species. It is therefore recommended by this paper as representing the most suitable barcode marker for Hoplolaimus species identification. This integrative study also resulted in the corrective reassignment of 17 gene sequences that were previously unidentified or incorrectly classified, as well as concluding that H. pararobustus consists of two cryptic species. 2023-02-01 2023-09-25T09:36:26Z 2023-09-25T09:36:26Z Journal Article https://hdl.handle.net/10568/131990 en Open Access application/pdf Walter de Gruyter GmbH Olajide, E., Singh, P.R., Kolombia, Y.A., Rumbarar, M.K., Couvreur, M. & Bert, W. (2023). Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus. Journal of Nematology, 55(1), 1-24. |
| spellingShingle | bananas hoplolaimus nematodes morphology phylogeny plant nematodes taxonomy dna Olajide, E. Singh, P.R. Kolombia, Y.A. Rumbarar, M.K. Couvreur, M. Bert, W. Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_full | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_fullStr | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_full_unstemmed | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_short | Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus |
| title_sort | characterization of hoplolaimus seinhorsti and hoplolaimus pararobustus tylenchina hoplolaimidae from banana with phylogeny and species delineation in the genus hoplolaimus |
| topic | bananas hoplolaimus nematodes morphology phylogeny plant nematodes taxonomy dna |
| url | https://hdl.handle.net/10568/131990 |
| work_keys_str_mv | AT olajidee characterizationofhoplolaimusseinhorstiandhoplolaimuspararobustustylenchinahoplolaimidaefrombananawithphylogenyandspeciesdelineationinthegenushoplolaimus AT singhpr characterizationofhoplolaimusseinhorstiandhoplolaimuspararobustustylenchinahoplolaimidaefrombananawithphylogenyandspeciesdelineationinthegenushoplolaimus AT kolombiaya characterizationofhoplolaimusseinhorstiandhoplolaimuspararobustustylenchinahoplolaimidaefrombananawithphylogenyandspeciesdelineationinthegenushoplolaimus AT rumbararmk characterizationofhoplolaimusseinhorstiandhoplolaimuspararobustustylenchinahoplolaimidaefrombananawithphylogenyandspeciesdelineationinthegenushoplolaimus AT couvreurm characterizationofhoplolaimusseinhorstiandhoplolaimuspararobustustylenchinahoplolaimidaefrombananawithphylogenyandspeciesdelineationinthegenushoplolaimus AT bertw characterizationofhoplolaimusseinhorstiandhoplolaimuspararobustustylenchinahoplolaimidaefrombananawithphylogenyandspeciesdelineationinthegenushoplolaimus |