Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids
The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance. In this study, we combined the recombinase-aided amplifi...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
American Chemical Society
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/131922 |
| _version_ | 1855521966049460224 |
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| author | Zhou, Y. Zhang, J. Sun, H. Tao, D. Xu, B. Han, X. Ren, R. Ruan, J. Steinaa, Lucilla Hemmink, Johanneke D. Han Jianlin Li, X. Xu, J. Zhao, S. Xie, S. Zhao, C. |
| author_browse | Han Jianlin Han, X. Hemmink, Johanneke D. Li, X. Ren, R. Ruan, J. Steinaa, Lucilla Sun, H. Tao, D. Xie, S. Xu, B. Xu, J. Zhang, J. Zhao, C. Zhao, S. Zhou, Y. |
| author_facet | Zhou, Y. Zhang, J. Sun, H. Tao, D. Xu, B. Han, X. Ren, R. Ruan, J. Steinaa, Lucilla Hemmink, Johanneke D. Han Jianlin Li, X. Xu, J. Zhao, S. Xie, S. Zhao, C. |
| author_sort | Zhou, Y. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance. In this study, we combined the recombinase-aided amplification (RAA) with the exonuclease III (Exo III)-assisted signal amplification into a platform for sensitive and specific detection of nucleic acids of African swine fever virus (ASFV). We found that this platform enabled a naked eye visual detection of ASFV at a detection limit as low as 2 copies/μL in 30 min. As expected, no cross-reactivity was observed with other porcine viruses. In addition, to avoid aerosol contamination, a one-tube RAA-Exo III colorimetric assay was also established for the accurate detection of ASFV in clinical samples. Taken together, we developed a rapid, instrument-free, and low-cost Exo III-assisted RAA colorimetric-assay-based nucleic acid detection platform. |
| format | Journal Article |
| id | CGSpace131922 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | American Chemical Society |
| publisherStr | American Chemical Society |
| record_format | dspace |
| spelling | CGSpace1319222024-07-29T16:33:36Z Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids Zhou, Y. Zhang, J. Sun, H. Tao, D. Xu, B. Han, X. Ren, R. Ruan, J. Steinaa, Lucilla Hemmink, Johanneke D. Han Jianlin Li, X. Xu, J. Zhao, S. Xie, S. Zhao, C. african swine fever virus diagnostic techniques The development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance. In this study, we combined the recombinase-aided amplification (RAA) with the exonuclease III (Exo III)-assisted signal amplification into a platform for sensitive and specific detection of nucleic acids of African swine fever virus (ASFV). We found that this platform enabled a naked eye visual detection of ASFV at a detection limit as low as 2 copies/μL in 30 min. As expected, no cross-reactivity was observed with other porcine viruses. In addition, to avoid aerosol contamination, a one-tube RAA-Exo III colorimetric assay was also established for the accurate detection of ASFV in clinical samples. Taken together, we developed a rapid, instrument-free, and low-cost Exo III-assisted RAA colorimetric-assay-based nucleic acid detection platform. 2023-10-20 2023-09-21T11:55:23Z 2023-09-21T11:55:23Z Journal Article https://hdl.handle.net/10568/131922 en Limited Access American Chemical Society Zhou, Y., Zhang, J., Sun, H., Tao, D., Xu, B., Han, X., Ren, R., Ruan, J., Steinaa, L., Hemmink, J.D., Han, J., Li, X., Xu, J., Zhao, S., Xie, S. and Zhao, C. 2023. Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids. ACS Synthetic Biology 12(10): 2877–2886. |
| spellingShingle | african swine fever virus diagnostic techniques Zhou, Y. Zhang, J. Sun, H. Tao, D. Xu, B. Han, X. Ren, R. Ruan, J. Steinaa, Lucilla Hemmink, Johanneke D. Han Jianlin Li, X. Xu, J. Zhao, S. Xie, S. Zhao, C. Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids |
| title | Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids |
| title_full | Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids |
| title_fullStr | Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids |
| title_full_unstemmed | Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids |
| title_short | Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids |
| title_sort | sensitive and specific exonuclease iii assisted recombinase aided amplification colorimetric assay for rapid detection of nucleic acids |
| topic | african swine fever virus diagnostic techniques |
| url | https://hdl.handle.net/10568/131922 |
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