Evidence that an unnamed isometric virus associated with potato rugose disease in Peru is a new species of torradovirus
A previously uncharacterized torradovirus species infecting potatoes was detected by high-throughput sequencing from field samples from Peru and in customs intercepts in potato tubers originated from South America in the USA and the Netherlands. This new potato torradovirus showed high nucleotide se...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Scientific Societies
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/131403 |
| _version_ | 1855517629325770752 |
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| author | Álvarez, R.A. Amao, M. Muller, G. Fuentes, S. Grinstead, S. Fuentes-Bueno, I. Roenhorst, A. Westenberg, M. Botermans, M. Kreuze, Jan F. Mollov, D. |
| author_browse | Amao, M. Botermans, M. Fuentes, S. Fuentes-Bueno, I. Grinstead, S. Kreuze, Jan F. Mollov, D. Muller, G. Roenhorst, A. Westenberg, M. Álvarez, R.A. |
| author_facet | Álvarez, R.A. Amao, M. Muller, G. Fuentes, S. Grinstead, S. Fuentes-Bueno, I. Roenhorst, A. Westenberg, M. Botermans, M. Kreuze, Jan F. Mollov, D. |
| author_sort | Álvarez, R.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A previously uncharacterized torradovirus species infecting potatoes was detected by high-throughput sequencing from field samples from Peru and in customs intercepts in potato tubers originated from South America in the USA and the Netherlands. This new potato torradovirus showed high nucleotide sequence identity to an unidentified isometric virus(SB26/29), which was associated with a disease named potato rugose stunting in southern Peru characterized over two decades ago. Thus, this virus is tentatively named potato rugose stunting virus (PotRSV). The genome of PotRSV isolates sequenced in this study were composed of two polyadenylated RNA segments. RNA1 ranges between 7,086-7,089 nt and RNA2 from 5,228 to 5230 nt. RNA1 encodes a polyprotein containing the replication block (Helicase-Protease-Polymerase), whereas RNA2 encodes a polyprotein cleaved into a movement protein and the three capsid proteins (CPs). Pairwise comparison among PotRSV isolates revealed amino acid identity values >86% in the protease-polymerase (Pro-Pol) region and >82% for the combined CPs. The closest torradovirus species, squash chlorotic leaf spot virus, shares amino acid identities of ~58% and ~41% in the Pro-Pol and the combined CPs, respectively. |
| format | Journal Article |
| id | CGSpace131403 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace1314032025-10-26T12:56:47Z Evidence that an unnamed isometric virus associated with potato rugose disease in Peru is a new species of torradovirus Álvarez, R.A. Amao, M. Muller, G. Fuentes, S. Grinstead, S. Fuentes-Bueno, I. Roenhorst, A. Westenberg, M. Botermans, M. Kreuze, Jan F. Mollov, D. epidemiology virology potatoes A previously uncharacterized torradovirus species infecting potatoes was detected by high-throughput sequencing from field samples from Peru and in customs intercepts in potato tubers originated from South America in the USA and the Netherlands. This new potato torradovirus showed high nucleotide sequence identity to an unidentified isometric virus(SB26/29), which was associated with a disease named potato rugose stunting in southern Peru characterized over two decades ago. Thus, this virus is tentatively named potato rugose stunting virus (PotRSV). The genome of PotRSV isolates sequenced in this study were composed of two polyadenylated RNA segments. RNA1 ranges between 7,086-7,089 nt and RNA2 from 5,228 to 5230 nt. RNA1 encodes a polyprotein containing the replication block (Helicase-Protease-Polymerase), whereas RNA2 encodes a polyprotein cleaved into a movement protein and the three capsid proteins (CPs). Pairwise comparison among PotRSV isolates revealed amino acid identity values >86% in the protease-polymerase (Pro-Pol) region and >82% for the combined CPs. The closest torradovirus species, squash chlorotic leaf spot virus, shares amino acid identities of ~58% and ~41% in the Pro-Pol and the combined CPs, respectively. 2023-09 2023-08-04T21:05:20Z 2023-08-04T21:05:20Z Journal Article https://hdl.handle.net/10568/131403 en Open Access Scientific Societies Alvarez, R.A.; Amao, M.; Muller, G.; Fuentes, S.; Grinstead, S.; Fuentes-Bueno, I.; Roenhorst, A.; Westenberg, M.; Botermans, M.; Kreuze, J.F.; Mollov, D. 2023. Evidence that an unnamed isometric virus associated with potato rugose disease in Peru is a new species of torradovirus. Phytopathology. ISSN 1943-7684. DOI: 10.1094/PHYTO-11-22-0449-V |
| spellingShingle | epidemiology virology potatoes Álvarez, R.A. Amao, M. Muller, G. Fuentes, S. Grinstead, S. Fuentes-Bueno, I. Roenhorst, A. Westenberg, M. Botermans, M. Kreuze, Jan F. Mollov, D. Evidence that an unnamed isometric virus associated with potato rugose disease in Peru is a new species of torradovirus |
| title | Evidence that an unnamed isometric virus associated with potato rugose disease in Peru is a new species of torradovirus |
| title_full | Evidence that an unnamed isometric virus associated with potato rugose disease in Peru is a new species of torradovirus |
| title_fullStr | Evidence that an unnamed isometric virus associated with potato rugose disease in Peru is a new species of torradovirus |
| title_full_unstemmed | Evidence that an unnamed isometric virus associated with potato rugose disease in Peru is a new species of torradovirus |
| title_short | Evidence that an unnamed isometric virus associated with potato rugose disease in Peru is a new species of torradovirus |
| title_sort | evidence that an unnamed isometric virus associated with potato rugose disease in peru is a new species of torradovirus |
| topic | epidemiology virology potatoes |
| url | https://hdl.handle.net/10568/131403 |
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