Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes
The root-burrowing nematode, Radopholus similis, is reputedly the most damaging nematode pest of banana and responsible for major production losses. In this study, the endophytic potential of 13 fungal isolates was assessed for the management of R. similis in East African Highland bananas (‘Ng’ombe’...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Brill
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/131364 |
| _version_ | 1855514360360730624 |
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| author | Kisaakye, J. Fourie, H. Coyne, D. Cortada, L. Khamis, F.M. Subramanian, S. Masinde, S. Haukeland, Solveig |
| author_browse | Cortada, L. Coyne, D. Fourie, H. Haukeland, Solveig Khamis, F.M. Kisaakye, J. Masinde, S. Subramanian, S. |
| author_facet | Kisaakye, J. Fourie, H. Coyne, D. Cortada, L. Khamis, F.M. Subramanian, S. Masinde, S. Haukeland, Solveig |
| author_sort | Kisaakye, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The root-burrowing nematode, Radopholus similis, is reputedly the most damaging nematode pest of banana and responsible for major production losses. In this study, the endophytic potential of 13 fungal isolates was assessed for the management of R. similis in East African Highland bananas (‘Ng’ombe’). All isolates successfully colonised tissue-cultured banana roots, with isolates from Trichoderma, Fusarium and Hypocrea producing the highest (⩾49.1%) and Beauveria isolates the lowest (⩽14.4%) colonisation. The fungal endophytes T. asperellum (ICIPE 700) and H. lixii (ICIPE 697) were the most effective in reducing R. similis densities (>81%) relative to the non-inoculated control. However, the combined inoculation of ICIPE 700 and ICIPE 697 led to greater suppression of R. similis (>21%) relative to individual inoculation. Suppression of R. similis following inoculation of banana roots with ICIPE 700 and/or ICIPE 697 was associated with the significant upregulation of the defence-related gene PR-1, the cell signalling gene calmodulin Ca2+ and the cell-wall-strengthening gene β-1,3-glucan synthase. This study demonstrates the potential for nematode management in bananas with fungal endophytes, especially using the isolates ICIPE 700 and ICIPE 697 when combined. |
| format | Journal Article |
| id | CGSpace131364 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Brill |
| publisherStr | Brill |
| record_format | dspace |
| spelling | CGSpace1313642025-12-08T10:11:39Z Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes Kisaakye, J. Fourie, H. Coyne, D. Cortada, L. Khamis, F.M. Subramanian, S. Masinde, S. Haukeland, Solveig biological control endophytes gene expression radopholus similis tissue culture bananas The root-burrowing nematode, Radopholus similis, is reputedly the most damaging nematode pest of banana and responsible for major production losses. In this study, the endophytic potential of 13 fungal isolates was assessed for the management of R. similis in East African Highland bananas (‘Ng’ombe’). All isolates successfully colonised tissue-cultured banana roots, with isolates from Trichoderma, Fusarium and Hypocrea producing the highest (⩾49.1%) and Beauveria isolates the lowest (⩽14.4%) colonisation. The fungal endophytes T. asperellum (ICIPE 700) and H. lixii (ICIPE 697) were the most effective in reducing R. similis densities (>81%) relative to the non-inoculated control. However, the combined inoculation of ICIPE 700 and ICIPE 697 led to greater suppression of R. similis (>21%) relative to individual inoculation. Suppression of R. similis following inoculation of banana roots with ICIPE 700 and/or ICIPE 697 was associated with the significant upregulation of the defence-related gene PR-1, the cell signalling gene calmodulin Ca2+ and the cell-wall-strengthening gene β-1,3-glucan synthase. This study demonstrates the potential for nematode management in bananas with fungal endophytes, especially using the isolates ICIPE 700 and ICIPE 697 when combined. 2023-03-02 2023-08-01T09:53:48Z 2023-08-01T09:53:48Z Journal Article https://hdl.handle.net/10568/131364 en Open Access application/pdf Brill Kisaakye, J., Fourie, H., Coyne, D., Cortada, L., Khamis, F.M., Subramanian, S., ... & Haukeland, S. (2023). Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes. Nematology, 25(4), 427-442. |
| spellingShingle | biological control endophytes gene expression radopholus similis tissue culture bananas Kisaakye, J. Fourie, H. Coyne, D. Cortada, L. Khamis, F.M. Subramanian, S. Masinde, S. Haukeland, Solveig Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes |
| title | Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes |
| title_full | Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes |
| title_fullStr | Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes |
| title_full_unstemmed | Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes |
| title_short | Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes |
| title_sort | endophytic fungi improve management of the burrowing nematode in banana musa spp through enhanced expression of defence related genes |
| topic | biological control endophytes gene expression radopholus similis tissue culture bananas |
| url | https://hdl.handle.net/10568/131364 |
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