Effectiveness of R1-nj anthocyanin marker in the identification of in vivo induced maize haploid embryos
Doubled haploid (DH) technology has become integral to maize breeding programs to expedite inbred line development and increase the efficiency of breeding operations. Unlike many other plant species that use in vitro methods, DH production in maize uses a relatively simple and efficient in vivo hapl...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
MDPI
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/131234 |
| _version_ | 1855541950053089280 |
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| author | López, Luis Antonio Ochieng, John Pacheco, Mario Martinez, Leocadio Hamilton Amoshe Omar Gowda, Manje Prasanna, Boddupalli M. Dhugga, Kanwarpal S. Chaikam, Vijay |
| author_browse | Chaikam, Vijay Dhugga, Kanwarpal S. Gowda, Manje Hamilton Amoshe Omar López, Luis Antonio Martinez, Leocadio Ochieng, John Pacheco, Mario Prasanna, Boddupalli M. |
| author_facet | López, Luis Antonio Ochieng, John Pacheco, Mario Martinez, Leocadio Hamilton Amoshe Omar Gowda, Manje Prasanna, Boddupalli M. Dhugga, Kanwarpal S. Chaikam, Vijay |
| author_sort | López, Luis Antonio |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Doubled haploid (DH) technology has become integral to maize breeding programs to expedite inbred line development and increase the efficiency of breeding operations. Unlike many other plant species that use in vitro methods, DH production in maize uses a relatively simple and efficient in vivo haploid induction method. However, it takes two complete crop cycles for DH line generation, one for haploid induction and the other one for chromosome doubling and seed production. Rescuing in vivo induced haploid embryos has the potential to reduce the time for DH line development and improve the efficiency of DH line production. However, the identification of a few haploid embryos (~10%) resulting from an induction cross from the rest of the diploid embryos is a challenge. In this study, we demonstrated that an anthocyanin marker, namely R1-nj, which is integrated into most haploid inducers, can aid in distinguishing haploid and diploid embryos. Further, we tested conditions that enhance R1-nj anthocyanin marker expression in embryos and found that light and sucrose enhance anthocyanin expression, while phosphorous deprivation in the media had no affect. Validating the use of the R1-nj marker for haploid and diploid embryo identification using a gold standard classification based on visual differences among haploids and diploids for characteristics such as seedling vigor, erectness of leaves, tassel fertility, etc., indicated that the R1-nj marker could lead to significantly high false positives, necessitating the use of additional markers for increased accuracy and reliability of haploid embryo identification. |
| format | Journal Article |
| id | CGSpace131234 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1312342025-12-08T10:29:22Z Effectiveness of R1-nj anthocyanin marker in the identification of in vivo induced maize haploid embryos López, Luis Antonio Ochieng, John Pacheco, Mario Martinez, Leocadio Hamilton Amoshe Omar Gowda, Manje Prasanna, Boddupalli M. Dhugga, Kanwarpal S. Chaikam, Vijay doubled haploids maize breeding programmes inbred lines crops Doubled haploid (DH) technology has become integral to maize breeding programs to expedite inbred line development and increase the efficiency of breeding operations. Unlike many other plant species that use in vitro methods, DH production in maize uses a relatively simple and efficient in vivo haploid induction method. However, it takes two complete crop cycles for DH line generation, one for haploid induction and the other one for chromosome doubling and seed production. Rescuing in vivo induced haploid embryos has the potential to reduce the time for DH line development and improve the efficiency of DH line production. However, the identification of a few haploid embryos (~10%) resulting from an induction cross from the rest of the diploid embryos is a challenge. In this study, we demonstrated that an anthocyanin marker, namely R1-nj, which is integrated into most haploid inducers, can aid in distinguishing haploid and diploid embryos. Further, we tested conditions that enhance R1-nj anthocyanin marker expression in embryos and found that light and sucrose enhance anthocyanin expression, while phosphorous deprivation in the media had no affect. Validating the use of the R1-nj marker for haploid and diploid embryo identification using a gold standard classification based on visual differences among haploids and diploids for characteristics such as seedling vigor, erectness of leaves, tassel fertility, etc., indicated that the R1-nj marker could lead to significantly high false positives, necessitating the use of additional markers for increased accuracy and reliability of haploid embryo identification. 2023 2023-07-20T20:39:36Z 2023-07-20T20:39:36Z Journal Article https://hdl.handle.net/10568/131234 en Open Access application/pdf MDPI Lopez, L. A., Ochieng, J., Pacheco, M., Martinez, L., Omar, H. A., Gowda, M., Prasanna, B. M., Dhugga, K. S., & Chaikam, V. (2023). Effectiveness of R1-nj Anthocyanin Marker in the Identification of In Vivo Induced Maize Haploid Embryos. Plants, 12(12), 2314. https://doi.org/10.3390/plants12122314 |
| spellingShingle | doubled haploids maize breeding programmes inbred lines crops López, Luis Antonio Ochieng, John Pacheco, Mario Martinez, Leocadio Hamilton Amoshe Omar Gowda, Manje Prasanna, Boddupalli M. Dhugga, Kanwarpal S. Chaikam, Vijay Effectiveness of R1-nj anthocyanin marker in the identification of in vivo induced maize haploid embryos |
| title | Effectiveness of R1-nj anthocyanin marker in the identification of in vivo induced maize haploid embryos |
| title_full | Effectiveness of R1-nj anthocyanin marker in the identification of in vivo induced maize haploid embryos |
| title_fullStr | Effectiveness of R1-nj anthocyanin marker in the identification of in vivo induced maize haploid embryos |
| title_full_unstemmed | Effectiveness of R1-nj anthocyanin marker in the identification of in vivo induced maize haploid embryos |
| title_short | Effectiveness of R1-nj anthocyanin marker in the identification of in vivo induced maize haploid embryos |
| title_sort | effectiveness of r1 nj anthocyanin marker in the identification of in vivo induced maize haploid embryos |
| topic | doubled haploids maize breeding programmes inbred lines crops |
| url | https://hdl.handle.net/10568/131234 |
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