Development of a naked eye CRISPR-Cas12a and -Cas13a multiplex point-of-care detection of genetically modified swine
The Rapid Visual CRISPR (RAVI-CRISPR) assay employs Cas12a and Cas13a enzymes for precise gene detection in a sample. However, RAVI-CRISPR is limited in single-tube multiplex detection applications due to the lack of specific single-strand (ss) DNA-fluorescently quenched (ssDNA-FQ) and RNA-fluoresce...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Chemical Society
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/131143 |
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