Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda
Introduction: Brucellosis is endemic in Uganda and is a major cause of production losses in livestock. Early detection and quantification of the disease is vital for its control and eradication. The aim of this study was to assess the sero-prevalence and factors associated with anti-Brucella antibod...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Frontiers Media
2023
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| Acceso en línea: | https://hdl.handle.net/10568/130947 |
| _version_ | 1855536516003004416 |
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| author | Bugeza, James Roesel, Kristina Moriyon, I. Mugizi, Denis Alinaitwe, Lordrick Kivali, Velma Kankya, C. Cook, Elizabeth A.J. |
| author_browse | Alinaitwe, Lordrick Bugeza, James Cook, Elizabeth A.J. Kankya, C. Kivali, Velma Moriyon, I. Mugizi, Denis Roesel, Kristina |
| author_facet | Bugeza, James Roesel, Kristina Moriyon, I. Mugizi, Denis Alinaitwe, Lordrick Kivali, Velma Kankya, C. Cook, Elizabeth A.J. |
| author_sort | Bugeza, James |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Introduction: Brucellosis is endemic in Uganda and is a major cause of production losses in livestock. Early detection and quantification of the disease is vital for its control and eradication. The aim of this study was to assess the sero-prevalence and factors associated with anti-Brucella antibodies in slaughtered livestock.
Materials and methods: Sera from 886 cattle, 925 small ruminants, and 900 pigs were collected from regional abattoirs in Northern, Eastern and Central Uganda. To estimate sero-prevalence, sera were serially tested using a combination of the Rose Bengal Test (RBT) and Native Hapten (NH) immunoprecipitation test. True sero-prevalence was estimated using the Rogan-Gladden estimator considering the sensitivity and specificity of the NH immunoprecipitation test. Multivariable logistic regression was used to assess factors associated with seropositivity for anti-Brucella antibodies.
Results and discussion: Small ruminants showed the highest seroprevalence (6.7%, 95% CI = 4.2-7.1) followed by cattle (3.8%, 95% CI = 2.4-4.9) and pigs (2.8%, 95% CI = 1.1-2.9). Seropositivity for anti-Brucella antibodies was associated with region of origin (OR = 4.6,95%CI=1.49-17.75, p = 0.013) for cattle; sex (OR = 2.90, 95% C = 1.5-6.34, p = 0.004), age (OR=4.04, 95% CI = 1.07-8.52, p = 0.006) and species (OR = 2.53, 95% CI = 1.08-6.98, p = 0.048) for small ruminants; and finally sex for pigs (OR = 2.88, 95% CI = 1.07-8.52, p = 0.041). Progressive control interventions must include both cattle and small ruminants since they play a bigger role in the maintenance and dissemination of Brucella. The interventions should adopt a risk-based approach with regions at higher risk being given top priority. Bacteriological and molecular studies should be undertaken to clarify the role of pigs and the goat-cattle cross infections in the epidemiological cycle of brucellosis in Uganda. |
| format | Journal Article |
| id | CGSpace130947 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1309472025-12-08T10:29:22Z Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda Bugeza, James Roesel, Kristina Moriyon, I. Mugizi, Denis Alinaitwe, Lordrick Kivali, Velma Kankya, C. Cook, Elizabeth A.J. brucellosis brucella livestock zoonoses Introduction: Brucellosis is endemic in Uganda and is a major cause of production losses in livestock. Early detection and quantification of the disease is vital for its control and eradication. The aim of this study was to assess the sero-prevalence and factors associated with anti-Brucella antibodies in slaughtered livestock. Materials and methods: Sera from 886 cattle, 925 small ruminants, and 900 pigs were collected from regional abattoirs in Northern, Eastern and Central Uganda. To estimate sero-prevalence, sera were serially tested using a combination of the Rose Bengal Test (RBT) and Native Hapten (NH) immunoprecipitation test. True sero-prevalence was estimated using the Rogan-Gladden estimator considering the sensitivity and specificity of the NH immunoprecipitation test. Multivariable logistic regression was used to assess factors associated with seropositivity for anti-Brucella antibodies. Results and discussion: Small ruminants showed the highest seroprevalence (6.7%, 95% CI = 4.2-7.1) followed by cattle (3.8%, 95% CI = 2.4-4.9) and pigs (2.8%, 95% CI = 1.1-2.9). Seropositivity for anti-Brucella antibodies was associated with region of origin (OR = 4.6,95%CI=1.49-17.75, p = 0.013) for cattle; sex (OR = 2.90, 95% C = 1.5-6.34, p = 0.004), age (OR=4.04, 95% CI = 1.07-8.52, p = 0.006) and species (OR = 2.53, 95% CI = 1.08-6.98, p = 0.048) for small ruminants; and finally sex for pigs (OR = 2.88, 95% CI = 1.07-8.52, p = 0.041). Progressive control interventions must include both cattle and small ruminants since they play a bigger role in the maintenance and dissemination of Brucella. The interventions should adopt a risk-based approach with regions at higher risk being given top priority. Bacteriological and molecular studies should be undertaken to clarify the role of pigs and the goat-cattle cross infections in the epidemiological cycle of brucellosis in Uganda. 2023-06-29 2023-06-29T17:53:40Z 2023-06-29T17:53:40Z Journal Article https://hdl.handle.net/10568/130947 en Open Access Frontiers Media Bugeza, J., Roesel, K., Moriyon, I., Mugizi, D., Alinaitwe, L., Kivali, V., Kankya, C. and Cook, E.A.J. 2023. Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda. Frontiers in Epidemiology 3: 1213592. |
| spellingShingle | brucellosis brucella livestock zoonoses Bugeza, James Roesel, Kristina Moriyon, I. Mugizi, Denis Alinaitwe, Lordrick Kivali, Velma Kankya, C. Cook, Elizabeth A.J. Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda |
| title | Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda |
| title_full | Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda |
| title_fullStr | Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda |
| title_full_unstemmed | Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda |
| title_short | Sero-prevalence and factors associated with anti-Brucella antibodies in slaughter livestock in Uganda |
| title_sort | sero prevalence and factors associated with anti brucella antibodies in slaughter livestock in uganda |
| topic | brucellosis brucella livestock zoonoses |
| url | https://hdl.handle.net/10568/130947 |
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