Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces
Cassava (Manihot esculenta Crantz) is a predominant food security crop in several developing countries. Its storage roots, rich in carbohydrate, are deficient in essential micronutrients, including provitamin A carotenoids. Increasing carotenoid content in cassava storage roots is important to redu...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/130477 |
| _version_ | 1855515934528110592 |
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| author | Olayide, P. Alexandersson, E. Tzfadia, O. Lenman, M. Gisel, A. Stavolone, L. |
| author_browse | Alexandersson, E. Gisel, A. Lenman, M. Olayide, P. Stavolone, L. Tzfadia, O. |
| author_facet | Olayide, P. Alexandersson, E. Tzfadia, O. Lenman, M. Gisel, A. Stavolone, L. |
| author_sort | Olayide, P. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava (Manihot esculenta Crantz) is a predominant food security crop in several developing countries. Its storage roots, rich in carbohydrate, are deficient in essential micronutrients, including provitamin A carotenoids.
Increasing carotenoid content in cassava storage roots is important to reduce the incidence of vitamin A deficiency, a public health problem in sub-Saharan Africa. However, cassava improvement advances slowly, mainly due to limited information on the molecular factors influencing β-carotene accumulation in cassava.
To address this problem, we performed comparative transcriptomic and untargeted metabolic analyses of roots and leaves of eleven African cassava landraces ranging from white to deep yellow colour, to uncover regulators of carotenoid biosynthesis and accumulation with conserved function in yellow cassava roots.
Sequence analysis confirmed the presence of a mutation, known to influence β-carotene content, in PSY transcripts of deep yellow but not of pale yellow genotypes. We identified genes and metabolites with expression and accumulation levels significantly associated with β-carotene content. Particularly an increased activity of the abscisic acid catabolism pathway together with a reduced amount of L-carnitine, may be related to the carotenoid pathway flux, higher in yellow than in white storage roots. In fact, NCED_3.1 was specifically expressed at a lower level in all yellow genotypes suggesting that it could be a potential target for increasing carotenoid accumulation in cassava.
These results expand the knowledge on metabolite compositions and molecular mechanisms influencing carotenoid biosynthesis and accumulation in cassava and provide novel information for biotechnological applications and genetic improvement of cassava with high nutritional values. |
| format | Journal Article |
| id | CGSpace130477 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1304772025-11-11T10:04:09Z Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces Olayide, P. Alexandersson, E. Tzfadia, O. Lenman, M. Gisel, A. Stavolone, L. cassava carotenoids transcriptome metabolites food security sub-saharan africa genetics physiology Cassava (Manihot esculenta Crantz) is a predominant food security crop in several developing countries. Its storage roots, rich in carbohydrate, are deficient in essential micronutrients, including provitamin A carotenoids. Increasing carotenoid content in cassava storage roots is important to reduce the incidence of vitamin A deficiency, a public health problem in sub-Saharan Africa. However, cassava improvement advances slowly, mainly due to limited information on the molecular factors influencing β-carotene accumulation in cassava. To address this problem, we performed comparative transcriptomic and untargeted metabolic analyses of roots and leaves of eleven African cassava landraces ranging from white to deep yellow colour, to uncover regulators of carotenoid biosynthesis and accumulation with conserved function in yellow cassava roots. Sequence analysis confirmed the presence of a mutation, known to influence β-carotene content, in PSY transcripts of deep yellow but not of pale yellow genotypes. We identified genes and metabolites with expression and accumulation levels significantly associated with β-carotene content. Particularly an increased activity of the abscisic acid catabolism pathway together with a reduced amount of L-carnitine, may be related to the carotenoid pathway flux, higher in yellow than in white storage roots. In fact, NCED_3.1 was specifically expressed at a lower level in all yellow genotypes suggesting that it could be a potential target for increasing carotenoid accumulation in cassava. These results expand the knowledge on metabolite compositions and molecular mechanisms influencing carotenoid biosynthesis and accumulation in cassava and provide novel information for biotechnological applications and genetic improvement of cassava with high nutritional values. 2023-06 2023-05-24T08:43:26Z 2023-05-24T08:43:26Z Journal Article https://hdl.handle.net/10568/130477 en Open Access application/pdf Elsevier Olayide, P., Alexandersson, E., Tzfadia, O., Lenman, M., Gisel, A. & Stavolone, L. (2023). Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces. Plant Physiology and Biochemistry, 199: 107713, 1-12. |
| spellingShingle | cassava carotenoids transcriptome metabolites food security sub-saharan africa genetics physiology Olayide, P. Alexandersson, E. Tzfadia, O. Lenman, M. Gisel, A. Stavolone, L. Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces |
| title | Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces |
| title_full | Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces |
| title_fullStr | Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces |
| title_full_unstemmed | Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces |
| title_short | Transcriptome and metabolome profiling identify factors potentially involved in pro-vitamin A accumulation in cassava landraces |
| title_sort | transcriptome and metabolome profiling identify factors potentially involved in pro vitamin a accumulation in cassava landraces |
| topic | cassava carotenoids transcriptome metabolites food security sub-saharan africa genetics physiology |
| url | https://hdl.handle.net/10568/130477 |
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