Prediction of photosynthetic, biophysical, and biochemical traits in wheat canopies to reduce the phenotyping bottleneck

To achieve food security, it is necessary to increase crop radiation use efficiency (RUE) and yield through the enhancement of canopy photosynthesis to increase the availability of assimilates for the grain, but its study in the field is constrained by low throughput and the lack of integrative meas...

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Main Authors: Robles-Zazueta, Carlos A., Pinto Espinosa, Francisco, Molero, Gemma, Foulkes, John Michael, Reynolds, Matthew P., Murchie, Erik Harry
Format: Journal Article
Language:Inglés
Published: Frontiers Media 2022
Subjects:
Online Access:https://hdl.handle.net/10568/130066
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author Robles-Zazueta, Carlos A.
Pinto Espinosa, Francisco
Molero, Gemma
Foulkes, John Michael
Reynolds, Matthew P.
Murchie, Erik Harry
author_browse Foulkes, John Michael
Molero, Gemma
Murchie, Erik Harry
Pinto Espinosa, Francisco
Reynolds, Matthew P.
Robles-Zazueta, Carlos A.
author_facet Robles-Zazueta, Carlos A.
Pinto Espinosa, Francisco
Molero, Gemma
Foulkes, John Michael
Reynolds, Matthew P.
Murchie, Erik Harry
author_sort Robles-Zazueta, Carlos A.
collection Repository of Agricultural Research Outputs (CGSpace)
description To achieve food security, it is necessary to increase crop radiation use efficiency (RUE) and yield through the enhancement of canopy photosynthesis to increase the availability of assimilates for the grain, but its study in the field is constrained by low throughput and the lack of integrative measurements at canopy level. In this study, partial least squares regression (PLSR) was used with high-throughput phenotyping (HTP) data in spring wheat to build predictive models of photosynthetic, biophysical, and biochemical traits for the top, middle, and bottom layers of wheat canopies. The combined layer model predictions performed better than individual layer predictions with a significance as follows for photosynthesis R2 = 0.48, RMSE = 5.24 μmol m–2 s–1 and stomatal conductance: R2 = 0.36, RMSE = 0.14 mol m–2 s–1. The predictions of these traits from PLSR models upscaled to canopy level compared to field observations were statistically significant at initiation of booting (R2 = 0.3, p < 0.05; R2 = 0.29, p < 0.05) and at 7 days after anthesis (R2 = 0.15, p < 0.05; R2 = 0.65, p < 0.001). Using HTP allowed us to increase phenotyping capacity 30-fold compared to conventional phenotyping methods. This approach can be adapted to screen breeding progeny and genetic resources for RUE and to improve our understanding of wheat physiology by adding different layers of the canopy to physiological modeling.
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spelling CGSpace1300662025-12-08T10:29:22Z Prediction of photosynthetic, biophysical, and biochemical traits in wheat canopies to reduce the phenotyping bottleneck Robles-Zazueta, Carlos A. Pinto Espinosa, Francisco Molero, Gemma Foulkes, John Michael Reynolds, Matthew P. Murchie, Erik Harry photosynthesis breeding spring wheat food security To achieve food security, it is necessary to increase crop radiation use efficiency (RUE) and yield through the enhancement of canopy photosynthesis to increase the availability of assimilates for the grain, but its study in the field is constrained by low throughput and the lack of integrative measurements at canopy level. In this study, partial least squares regression (PLSR) was used with high-throughput phenotyping (HTP) data in spring wheat to build predictive models of photosynthetic, biophysical, and biochemical traits for the top, middle, and bottom layers of wheat canopies. The combined layer model predictions performed better than individual layer predictions with a significance as follows for photosynthesis R2 = 0.48, RMSE = 5.24 μmol m–2 s–1 and stomatal conductance: R2 = 0.36, RMSE = 0.14 mol m–2 s–1. The predictions of these traits from PLSR models upscaled to canopy level compared to field observations were statistically significant at initiation of booting (R2 = 0.3, p < 0.05; R2 = 0.29, p < 0.05) and at 7 days after anthesis (R2 = 0.15, p < 0.05; R2 = 0.65, p < 0.001). Using HTP allowed us to increase phenotyping capacity 30-fold compared to conventional phenotyping methods. This approach can be adapted to screen breeding progeny and genetic resources for RUE and to improve our understanding of wheat physiology by adding different layers of the canopy to physiological modeling. 2022-04-11 2023-04-20T14:54:19Z 2023-04-20T14:54:19Z Journal Article https://hdl.handle.net/10568/130066 en Open Access application/pdf Frontiers Media Robles-Zazueta, C. A., Pinto, F., Molero, G., Foulkes, M. J., Reynolds, M. P., & Murchie, E. H. (2022). Prediction of Photosynthetic, Biophysical, and Biochemical Traits in Wheat Canopies to Reduce the Phenotyping Bottleneck. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.828451
spellingShingle photosynthesis
breeding
spring wheat
food security
Robles-Zazueta, Carlos A.
Pinto Espinosa, Francisco
Molero, Gemma
Foulkes, John Michael
Reynolds, Matthew P.
Murchie, Erik Harry
Prediction of photosynthetic, biophysical, and biochemical traits in wheat canopies to reduce the phenotyping bottleneck
title Prediction of photosynthetic, biophysical, and biochemical traits in wheat canopies to reduce the phenotyping bottleneck
title_full Prediction of photosynthetic, biophysical, and biochemical traits in wheat canopies to reduce the phenotyping bottleneck
title_fullStr Prediction of photosynthetic, biophysical, and biochemical traits in wheat canopies to reduce the phenotyping bottleneck
title_full_unstemmed Prediction of photosynthetic, biophysical, and biochemical traits in wheat canopies to reduce the phenotyping bottleneck
title_short Prediction of photosynthetic, biophysical, and biochemical traits in wheat canopies to reduce the phenotyping bottleneck
title_sort prediction of photosynthetic biophysical and biochemical traits in wheat canopies to reduce the phenotyping bottleneck
topic photosynthesis
breeding
spring wheat
food security
url https://hdl.handle.net/10568/130066
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