Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population
Pyrenophora tritici-repentis causes tan spot, an important foliar disease of wheat. A collection of P. tritici-repentis isolates from Tunisia, located in one of the main secondary centers of diversification of durum wheat, was tested for phenotypic race classification based on virulence on a host di...
| Autores principales: | , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
American Phytopathological Society
2022
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/129955 |
| _version_ | 1855523472463101952 |
|---|---|
| author | Laribi, Marwa Akhavan, Alireza Ben M’Barek, Sarrah Yahyaoui, Amor Hassine Strelkov, Stephen E. Sassi, Khaled |
| author_browse | Akhavan, Alireza Ben M’Barek, Sarrah Laribi, Marwa Sassi, Khaled Strelkov, Stephen E. Yahyaoui, Amor Hassine |
| author_facet | Laribi, Marwa Akhavan, Alireza Ben M’Barek, Sarrah Yahyaoui, Amor Hassine Strelkov, Stephen E. Sassi, Khaled |
| author_sort | Laribi, Marwa |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pyrenophora tritici-repentis causes tan spot, an important foliar disease of wheat. A collection of P. tritici-repentis isolates from Tunisia, located in one of the main secondary centers of diversification of durum wheat, was tested for phenotypic race classification based on virulence on a host differential set and for the presence of the necrotrophic effector (NE) genes ToxA, ToxB, and toxb by PCR analysis. While races 2, 4, 5, 6, 7, and 8 were identified according to their virulence phenotypes, PCR testing indicated the presence of “atypical” isolates that induced necrosis on the wheat differential ‘Glenlea,’ but lacked the expected ToxA gene, suggesting the involvement of other NEs in the P. tritici-repentis/wheat interaction. Genetic diversity and the P. tritici-repentis population structure were explored further by examining 59 Tunisian isolates and 35 isolates from Algeria, Azerbaijan, Canada, Iran, and Syria using 24 simple sequence repeat markers. Average genetic diversity, overall gene flow, and percentage polymorphic loci were estimated as 0.58, 2.09, and 87%, respectively. Analysis of molecular variance showed that 81% of the genetic variance occurred within populations and 19% occurred between populations. Cluster analysis by the unweighted pair group method indicated that ToxB– isolates grouped together and were distantly related to ToxB+ isolates. Based on Nei’s analysis, the global collection clustered into two distinct groups according to their region of origin. The results suggest that geographic origin and the host specificity imposed by different NEs can lead to differentiation among P. tritici-repentis populations. |
| format | Journal Article |
| id | CGSpace129955 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | CGSpace1299552024-08-27T10:35:46Z Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population Laribi, Marwa Akhavan, Alireza Ben M’Barek, Sarrah Yahyaoui, Amor Hassine Strelkov, Stephen E. Sassi, Khaled pyrenophora tritici repentis microsatellites foliar diseases wheat Pyrenophora tritici-repentis causes tan spot, an important foliar disease of wheat. A collection of P. tritici-repentis isolates from Tunisia, located in one of the main secondary centers of diversification of durum wheat, was tested for phenotypic race classification based on virulence on a host differential set and for the presence of the necrotrophic effector (NE) genes ToxA, ToxB, and toxb by PCR analysis. While races 2, 4, 5, 6, 7, and 8 were identified according to their virulence phenotypes, PCR testing indicated the presence of “atypical” isolates that induced necrosis on the wheat differential ‘Glenlea,’ but lacked the expected ToxA gene, suggesting the involvement of other NEs in the P. tritici-repentis/wheat interaction. Genetic diversity and the P. tritici-repentis population structure were explored further by examining 59 Tunisian isolates and 35 isolates from Algeria, Azerbaijan, Canada, Iran, and Syria using 24 simple sequence repeat markers. Average genetic diversity, overall gene flow, and percentage polymorphic loci were estimated as 0.58, 2.09, and 87%, respectively. Analysis of molecular variance showed that 81% of the genetic variance occurred within populations and 19% occurred between populations. Cluster analysis by the unweighted pair group method indicated that ToxB– isolates grouped together and were distantly related to ToxB+ isolates. Based on Nei’s analysis, the global collection clustered into two distinct groups according to their region of origin. The results suggest that geographic origin and the host specificity imposed by different NEs can lead to differentiation among P. tritici-repentis populations. 2022-02-01 2023-04-12T15:03:42Z 2023-04-12T15:03:42Z Journal Article https://hdl.handle.net/10568/129955 en Limited Access American Phytopathological Society Laribi, M., Akhavan, A., Ben M’Barek, S., Yahyaoui, A. H., Strelkov, S. E. and Sassi, K. 2022. Characterization of pyrenophora tritici-repentis in Tunisia and comparison with a global pathogen population. Plant Disease, 106(2), 464–474 |
| spellingShingle | pyrenophora tritici repentis microsatellites foliar diseases wheat Laribi, Marwa Akhavan, Alireza Ben M’Barek, Sarrah Yahyaoui, Amor Hassine Strelkov, Stephen E. Sassi, Khaled Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population |
| title | Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population |
| title_full | Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population |
| title_fullStr | Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population |
| title_full_unstemmed | Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population |
| title_short | Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population |
| title_sort | characterization of pyrenophora tritici repentis in tunisia and comparison with a global pathogen population |
| topic | pyrenophora tritici repentis microsatellites foliar diseases wheat |
| url | https://hdl.handle.net/10568/129955 |
| work_keys_str_mv | AT laribimarwa characterizationofpyrenophoratriticirepentisintunisiaandcomparisonwithaglobalpathogenpopulation AT akhavanalireza characterizationofpyrenophoratriticirepentisintunisiaandcomparisonwithaglobalpathogenpopulation AT benmbareksarrah characterizationofpyrenophoratriticirepentisintunisiaandcomparisonwithaglobalpathogenpopulation AT yahyaouiamorhassine characterizationofpyrenophoratriticirepentisintunisiaandcomparisonwithaglobalpathogenpopulation AT strelkovstephene characterizationofpyrenophoratriticirepentisintunisiaandcomparisonwithaglobalpathogenpopulation AT sassikhaled characterizationofpyrenophoratriticirepentisintunisiaandcomparisonwithaglobalpathogenpopulation |