Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties
Synthetic nitrification inhibitors (SNI) and biological nitrification inhibitors (BNI) are promising tools to limit nitrogen (N) pollution derived from agriculture. Modern wheat cultivars lack sufficient capacity to exude BNIs, but, fortunately, the chromosome region (Lr#n-SA) controlling BNI produc...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Frontiers Media
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/129906 |
| _version_ | 1855526994182144000 |
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| author | Bozal-Leorri, Adrián Guntur Venkata Subbarao Kishii, Masahiro Urmeneta, Leyre Kommerell, Víctor Karwat, Hannes Braun, Hans-Joachim Aparicio-Tejo, Pedro M. Ortíz Monasterio, Jose Iván González Murua, Carmen González Moro, M. Begoña |
| author_browse | Aparicio-Tejo, Pedro M. Bozal-Leorri, Adrián Braun, Hans-Joachim González Moro, M. Begoña González Murua, Carmen Guntur Venkata Subbarao Karwat, Hannes Kishii, Masahiro Kommerell, Víctor Ortíz Monasterio, Jose Iván Urmeneta, Leyre |
| author_facet | Bozal-Leorri, Adrián Guntur Venkata Subbarao Kishii, Masahiro Urmeneta, Leyre Kommerell, Víctor Karwat, Hannes Braun, Hans-Joachim Aparicio-Tejo, Pedro M. Ortíz Monasterio, Jose Iván González Murua, Carmen González Moro, M. Begoña |
| author_sort | Bozal-Leorri, Adrián |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Synthetic nitrification inhibitors (SNI) and biological nitrification inhibitors (BNI) are promising tools to limit nitrogen (N) pollution derived from agriculture. Modern wheat cultivars lack sufficient capacity to exude BNIs, but, fortunately, the chromosome region (Lr#n-SA) controlling BNI production in Leymus racemosus, a wild relative of wheat, was introduced into two elite wheat cultivars, ROELFS and MUNAL. Using BNI-isogenic-lines could become a cost-effective, farmer-friendly, and globally scalable technology that incentivizes more sustainable and environmentally friendly agronomic practices. We studied how BNI-trait improves N-uptake, and N-use, both with ammonium and nitrate fertilization, analysing representative indicators of soil nitrification inhibition, and plant metabolism. Synthesizing BNI molecules did not mean a metabolic cost since Control and BNI-isogenic-lines from ROELFS and MUNAL presented similar agronomic performance and plant development. In the soil, ROELFS-BNI and MUNAL-BNI plants decreased ammonia-oxidizing bacteria (AOB) abundance by 60% and 45% respectively, delaying ammonium oxidation without reducing the total abundance of bacteria or archaea. Interestingly, BNI-trait presented a synergistic effect with SNIs since made it also possible to decrease the AOA abundance. ROELFS-BNI and MUNAL-BNI plants showed a reduced leaf nitrate reductase (NR) activity as a consequence of lower soil (Formula presented.) formation and a higher amino acid content compared to BNI-trait lacking lines, indicating that the transfer of Lr#-SA was able to induce a higher capacity to assimilate ammonium. Moreover, the impact of the BNI-trait in wheat cultivars was also noticeable for nitrate fertilization, with improved N absorption, and therefore, reducing soil nitrate content. |
| format | Journal Article |
| id | CGSpace129906 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1299062025-12-08T10:29:22Z Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties Bozal-Leorri, Adrián Guntur Venkata Subbarao Kishii, Masahiro Urmeneta, Leyre Kommerell, Víctor Karwat, Hannes Braun, Hans-Joachim Aparicio-Tejo, Pedro M. Ortíz Monasterio, Jose Iván González Murua, Carmen González Moro, M. Begoña wheat nitrification nitrogen fertilizers ammonium carieties Synthetic nitrification inhibitors (SNI) and biological nitrification inhibitors (BNI) are promising tools to limit nitrogen (N) pollution derived from agriculture. Modern wheat cultivars lack sufficient capacity to exude BNIs, but, fortunately, the chromosome region (Lr#n-SA) controlling BNI production in Leymus racemosus, a wild relative of wheat, was introduced into two elite wheat cultivars, ROELFS and MUNAL. Using BNI-isogenic-lines could become a cost-effective, farmer-friendly, and globally scalable technology that incentivizes more sustainable and environmentally friendly agronomic practices. We studied how BNI-trait improves N-uptake, and N-use, both with ammonium and nitrate fertilization, analysing representative indicators of soil nitrification inhibition, and plant metabolism. Synthesizing BNI molecules did not mean a metabolic cost since Control and BNI-isogenic-lines from ROELFS and MUNAL presented similar agronomic performance and plant development. In the soil, ROELFS-BNI and MUNAL-BNI plants decreased ammonia-oxidizing bacteria (AOB) abundance by 60% and 45% respectively, delaying ammonium oxidation without reducing the total abundance of bacteria or archaea. Interestingly, BNI-trait presented a synergistic effect with SNIs since made it also possible to decrease the AOA abundance. ROELFS-BNI and MUNAL-BNI plants showed a reduced leaf nitrate reductase (NR) activity as a consequence of lower soil (Formula presented.) formation and a higher amino acid content compared to BNI-trait lacking lines, indicating that the transfer of Lr#-SA was able to induce a higher capacity to assimilate ammonium. Moreover, the impact of the BNI-trait in wheat cultivars was also noticeable for nitrate fertilization, with improved N absorption, and therefore, reducing soil nitrate content. 2022-11-11 2023-04-05T15:23:30Z 2023-04-05T15:23:30Z Journal Article https://hdl.handle.net/10568/129906 en Open Access application/pdf Frontiers Media Bozal-Leorri, A., Subbarao, G.V., Kishii, M., Urmeneta, L., Kommerell, V., Karwat, H., Braun, H.-J., Aparicio-Tejo, P.M., Ortiz-Monasterio, I., González-Murua, C. and González-Moro, M.B. 2022. Bio-logical nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and im-proves ammonium assimilation in two elite wheat varieties. Frontiers in Plant Science 13:1034219. |
| spellingShingle | wheat nitrification nitrogen fertilizers ammonium carieties Bozal-Leorri, Adrián Guntur Venkata Subbarao Kishii, Masahiro Urmeneta, Leyre Kommerell, Víctor Karwat, Hannes Braun, Hans-Joachim Aparicio-Tejo, Pedro M. Ortíz Monasterio, Jose Iván González Murua, Carmen González Moro, M. Begoña Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties |
| title | Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties |
| title_full | Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties |
| title_fullStr | Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties |
| title_full_unstemmed | Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties |
| title_short | Biological nitrification inhibitor-trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties |
| title_sort | biological nitrification inhibitor trait enhances nitrogen uptake by suppressing nitrifier activity and improves ammonium assimilation in two elite wheat varieties |
| topic | wheat nitrification nitrogen fertilizers ammonium carieties |
| url | https://hdl.handle.net/10568/129906 |
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