Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines
Grass pea ( Lathyrus sativus ) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP) in tissues of the plant...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2019
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/129608 |
| _version_ | 1855532474410467328 |
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| author | Emmrich, Peter M.F. Rejzek, Martin Hill, Lionel Brett, Paul Edwards, Anne Sarkar, Abhimanyu Field, Rob A. Martin, Cathie Wang, Trevor L. |
| author_browse | Brett, Paul Edwards, Anne Emmrich, Peter M.F. Field, Rob A. Hill, Lionel Martin, Cathie Rejzek, Martin Sarkar, Abhimanyu Wang, Trevor L. |
| author_facet | Emmrich, Peter M.F. Rejzek, Martin Hill, Lionel Brett, Paul Edwards, Anne Sarkar, Abhimanyu Field, Rob A. Martin, Cathie Wang, Trevor L. |
| author_sort | Emmrich, Peter M.F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Grass pea ( Lathyrus sativus ) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP) in tissues of the plant has limited its adoption as a staple crop. To assist in the detection of material with very low neurotoxin toxin levels, we have developed two novel methods to assay ODAP. The first, a version of a widely used spectrophotometric assay, modified for increased throughput, permits rapid screening of large populations of germplasm for low toxin lines and the second is a novel, mass spectrometric procedure to detect very small quantities of ODAP for research purposes and characterisation of new varieties. |
| format | Journal Article |
| id | CGSpace129608 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1296082024-08-27T06:52:57Z Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines Emmrich, Peter M.F. Rejzek, Martin Hill, Lionel Brett, Paul Edwards, Anne Sarkar, Abhimanyu Field, Rob A. Martin, Cathie Wang, Trevor L. identification permits pea Grass pea ( Lathyrus sativus ) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP) in tissues of the plant has limited its adoption as a staple crop. To assist in the detection of material with very low neurotoxin toxin levels, we have developed two novel methods to assay ODAP. The first, a version of a widely used spectrophotometric assay, modified for increased throughput, permits rapid screening of large populations of germplasm for low toxin lines and the second is a novel, mass spectrometric procedure to detect very small quantities of ODAP for research purposes and characterisation of new varieties. 2019-12 2023-03-10T14:41:21Z 2023-03-10T14:41:21Z Journal Article https://hdl.handle.net/10568/129608 en Open Access Springer Emmrich, Peter M.F.; Rejzek, Martin; Hill, Lionel; Brett, Paul; Edwards, Anne; Sarkar, Abhimanyu; Field, Rob A.; Martin, Cathie; Wang, Trevor L. 2019. Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines. BMC Plant Biology 19: |
| spellingShingle | identification permits pea Emmrich, Peter M.F. Rejzek, Martin Hill, Lionel Brett, Paul Edwards, Anne Sarkar, Abhimanyu Field, Rob A. Martin, Cathie Wang, Trevor L. Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines |
| title | Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines |
| title_full | Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines |
| title_fullStr | Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines |
| title_full_unstemmed | Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines |
| title_short | Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines |
| title_sort | linking a rapid throughput plate assay with high sensitivity stable isotope label lcms quantification permits the identification and characterisation of low β l odap grass pea lines |
| topic | identification permits pea |
| url | https://hdl.handle.net/10568/129608 |
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