Fine Mapping of the Subunit Binding Sites of Influenza Virus RNA Polymerase
Influenza virus RNA polymerase consists of three subunits, PB1, PB2 and PA, and catalyzes both transcription and replication of the RNA genome. PB1 is a catalytic subunit of RNA polymerization and a core of the subunit assembly. The subunit binding sites were mapped at about several hundred amino‐ac...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2002
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/129595 |
| _version_ | 1855529412647190528 |
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| author | Ohtsu, Yasushi Honda, Yoshikazu Sakata, Yasutaka Kato, Hirohisa Toyoda, Tetsuya |
| author_browse | Honda, Yoshikazu Kato, Hirohisa Ohtsu, Yasushi Sakata, Yasutaka Toyoda, Tetsuya |
| author_facet | Ohtsu, Yasushi Honda, Yoshikazu Sakata, Yasutaka Kato, Hirohisa Toyoda, Tetsuya |
| author_sort | Ohtsu, Yasushi |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Influenza virus RNA polymerase consists of three subunits, PB1, PB2 and PA, and catalyzes both transcription and replication of the RNA genome. PB1 is a catalytic subunit of RNA polymerization and a core of the subunit assembly. The subunit binding sites were mapped at about several hundred amino‐acid size. Fine mapping of the subunit binding sites was determined. The PB1‐PA binding regions were mapped within in the N‐terminal 25 amino acids of PB1 and 668–692 of PA. PB1 and PB2 interacted within wider regions, 600–757 of PB1 and 51–259 of PB2. In these amino‐acid spans, 206–259 of PB2 may be the most important region of PB1 binding and 718–732 of PB1 may be the most important region of PB2 binding because the binding activity was lost when the regions were lost in the subunits. The additional regions contributed to strong binding of these subunits. |
| format | Journal Article |
| id | CGSpace129595 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1295952024-11-15T08:52:40Z Fine Mapping of the Subunit Binding Sites of Influenza Virus RNA Polymerase Ohtsu, Yasushi Honda, Yoshikazu Sakata, Yasutaka Kato, Hirohisa Toyoda, Tetsuya mapping rna virus Influenza virus RNA polymerase consists of three subunits, PB1, PB2 and PA, and catalyzes both transcription and replication of the RNA genome. PB1 is a catalytic subunit of RNA polymerization and a core of the subunit assembly. The subunit binding sites were mapped at about several hundred amino‐acid size. Fine mapping of the subunit binding sites was determined. The PB1‐PA binding regions were mapped within in the N‐terminal 25 amino acids of PB1 and 668–692 of PA. PB1 and PB2 interacted within wider regions, 600–757 of PB1 and 51–259 of PB2. In these amino‐acid spans, 206–259 of PB2 may be the most important region of PB1 binding and 718–732 of PB1 may be the most important region of PB2 binding because the binding activity was lost when the regions were lost in the subunits. The additional regions contributed to strong binding of these subunits. 2002-03 2023-03-10T14:40:45Z 2023-03-10T14:40:45Z Journal Article https://hdl.handle.net/10568/129595 en Open Access Wiley Ohtsu, Yasushi; Honda, Yoshikazu; Sakata, Yasutaka; Kato, Hirohisa; Toyoda, Tetsuya. 2002. Fine Mapping of the Subunit Binding Sites of Influenza Virus RNA Polymerase. Microbiology and Immunology 46: 167-175 |
| spellingShingle | mapping rna virus Ohtsu, Yasushi Honda, Yoshikazu Sakata, Yasutaka Kato, Hirohisa Toyoda, Tetsuya Fine Mapping of the Subunit Binding Sites of Influenza Virus RNA Polymerase |
| title | Fine Mapping of the Subunit Binding Sites of Influenza Virus RNA Polymerase |
| title_full | Fine Mapping of the Subunit Binding Sites of Influenza Virus RNA Polymerase |
| title_fullStr | Fine Mapping of the Subunit Binding Sites of Influenza Virus RNA Polymerase |
| title_full_unstemmed | Fine Mapping of the Subunit Binding Sites of Influenza Virus RNA Polymerase |
| title_short | Fine Mapping of the Subunit Binding Sites of Influenza Virus RNA Polymerase |
| title_sort | fine mapping of the subunit binding sites of influenza virus rna polymerase |
| topic | mapping rna virus |
| url | https://hdl.handle.net/10568/129595 |
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