Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa
Understanding the genetic diversity of rice germplasm is important for the sustainable use of genetic materials in rice breeding and production. Africa is rich in rice genetic resources that can be utilized to boost rice productivity on the continent. A major constraint to rice production in Africa...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Scientific Societies
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/129575 |
| _version_ | 1855541181380820992 |
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| author | Mgonja, Emmanuel M. Park, Chan Ho Houxiang Kang Balimponya, Elias G. Opiyo, Stephen Bellizzi, Maria Mutiga, Samuel Kilonzo Rotich, Felix Ganeshan, Veena Devi Mabagala, Robert Sneller, Clay H. Correll, James C. Bo Zhou Talbot, Nicholas J. Mitchell, Thomas K. Guo-Liang Wang |
| author_browse | Balimponya, Elias G. Bellizzi, Maria Bo Zhou Correll, James C. Ganeshan, Veena Devi Guo-Liang Wang Houxiang Kang Mabagala, Robert Mgonja, Emmanuel M. Mitchell, Thomas K. Mutiga, Samuel Kilonzo Opiyo, Stephen Park, Chan Ho Rotich, Felix Sneller, Clay H. Talbot, Nicholas J. |
| author_facet | Mgonja, Emmanuel M. Park, Chan Ho Houxiang Kang Balimponya, Elias G. Opiyo, Stephen Bellizzi, Maria Mutiga, Samuel Kilonzo Rotich, Felix Ganeshan, Veena Devi Mabagala, Robert Sneller, Clay H. Correll, James C. Bo Zhou Talbot, Nicholas J. Mitchell, Thomas K. Guo-Liang Wang |
| author_sort | Mgonja, Emmanuel M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Understanding the genetic diversity of rice germplasm is important for the sustainable use of genetic materials in rice breeding and production. Africa is rich in rice genetic resources that can be utilized to boost rice productivity on the continent. A major constraint to rice production in Africa is rice blast, caused by the hemibiotrophic fungal pathogen Magnaporthe oryzae. In this report, we present the results of a genotyping-by-sequencing (GBS)-based diversity analysis of 190 African rice cultivars and an association mapping of blast resistance (R) genes and quantitative trait loci (QTLs). The 190 African cultivars were clustered into three groups based on the 184K single nucleotide polymorphisms generated by GBS. We inoculated the rice cultivars with six African M. oryzae isolates. Association mapping identified 25 genomic regions associated with blast resistance (RABRs) in the rice genome. Moreover, PCR analysis indicated that RABR_23 is associated with the Pi-ta gene on chromosome 12. Our study demonstrates that the combination of GBS-based genetic diversity population analysis and association mapping is effective in identifying rice blast R genes/QTLs that contribute to resistance against African populations of M. oryzae. The identified markers linked to the RABRs and 14 highly resistant cultivars in this study will be useful for rice breeding in Africa. |
| format | Journal Article |
| id | CGSpace129575 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace1295752024-08-27T10:37:17Z Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa Mgonja, Emmanuel M. Park, Chan Ho Houxiang Kang Balimponya, Elias G. Opiyo, Stephen Bellizzi, Maria Mutiga, Samuel Kilonzo Rotich, Felix Ganeshan, Veena Devi Mabagala, Robert Sneller, Clay H. Correll, James C. Bo Zhou Talbot, Nicholas J. Mitchell, Thomas K. Guo-Liang Wang rice cultivars genes mapping analysis africa genotyping magnaporthe african rice association Understanding the genetic diversity of rice germplasm is important for the sustainable use of genetic materials in rice breeding and production. Africa is rich in rice genetic resources that can be utilized to boost rice productivity on the continent. A major constraint to rice production in Africa is rice blast, caused by the hemibiotrophic fungal pathogen Magnaporthe oryzae. In this report, we present the results of a genotyping-by-sequencing (GBS)-based diversity analysis of 190 African rice cultivars and an association mapping of blast resistance (R) genes and quantitative trait loci (QTLs). The 190 African cultivars were clustered into three groups based on the 184K single nucleotide polymorphisms generated by GBS. We inoculated the rice cultivars with six African M. oryzae isolates. Association mapping identified 25 genomic regions associated with blast resistance (RABRs) in the rice genome. Moreover, PCR analysis indicated that RABR_23 is associated with the Pi-ta gene on chromosome 12. Our study demonstrates that the combination of GBS-based genetic diversity population analysis and association mapping is effective in identifying rice blast R genes/QTLs that contribute to resistance against African populations of M. oryzae. The identified markers linked to the RABRs and 14 highly resistant cultivars in this study will be useful for rice breeding in Africa. 2017-09 2023-03-10T14:39:54Z 2023-03-10T14:39:54Z Journal Article https://hdl.handle.net/10568/129575 en Open Access Scientific Societies Mgonja, Emmanuel M.; Park, Chan Ho; Kang, Houxiang; Balimponya, Elias G.; Opiyo, Stephen; Bellizzi, Maria; Mutiga, Samuel Kilonzo; Rotich, Felix; Ganeshan, Veena Devi; Mabagala, Robert; Sneller, Clay H.; Correll, James C.; Zhou, Bo; Talbot, Nicholas J.; Mitchell, Thomas K.; Wang, Guo-Liang. 2017. Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa. Phytopathology® 107: 1039-1046 |
| spellingShingle | rice cultivars genes mapping analysis africa genotyping magnaporthe african rice association Mgonja, Emmanuel M. Park, Chan Ho Houxiang Kang Balimponya, Elias G. Opiyo, Stephen Bellizzi, Maria Mutiga, Samuel Kilonzo Rotich, Felix Ganeshan, Veena Devi Mabagala, Robert Sneller, Clay H. Correll, James C. Bo Zhou Talbot, Nicholas J. Mitchell, Thomas K. Guo-Liang Wang Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa |
| title | Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa |
| title_full | Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa |
| title_fullStr | Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa |
| title_full_unstemmed | Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa |
| title_short | Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa |
| title_sort | genotyping by sequencing based genetic analysis of african rice cultivars and association mapping of blast resistance genes against magnaporthe oryzae populations in africa |
| topic | rice cultivars genes mapping analysis africa genotyping magnaporthe african rice association |
| url | https://hdl.handle.net/10568/129575 |
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