Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus
Grass pea ( Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and t...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/129553 |
| _version_ | 1855539295479136256 |
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| author | Edwards, Anne Njaci, Isaac Sarkar, Abhimanyu Jiang, Zhouqian Kaithakottil, Gemy George Moore, Christopher Cheema, Jitender Stevenson, Clare E.M. Rejzek, Martin Novák, Petr Vigouroux, Marielle Vickers, Martin Wouters, Roland H.M. Paajanen, Pirita Steuernagel, Burkhard Moore, Jonathan D. Higgins, Janet Swarbreck, David Martens, Stefan Kim, Colin Y. Weng, Jing-Ke Mundree, Sagadevan Kilian, Benjamin Kumar, Shiv Loose, Matt Yant, Levi Macas, Jiří Wang, Trevor L. Martin, Cathie Emmrich, Peter M.F. |
| author_browse | Cheema, Jitender Edwards, Anne Emmrich, Peter M.F. Higgins, Janet Jiang, Zhouqian Kaithakottil, Gemy George Kilian, Benjamin Kim, Colin Y. Kumar, Shiv Loose, Matt Macas, Jiří Martens, Stefan Martin, Cathie Moore, Christopher Moore, Jonathan D. Mundree, Sagadevan Njaci, Isaac Novák, Petr Paajanen, Pirita Rejzek, Martin Sarkar, Abhimanyu Steuernagel, Burkhard Stevenson, Clare E.M. Swarbreck, David Vickers, Martin Vigouroux, Marielle Wang, Trevor L. Weng, Jing-Ke Wouters, Roland H.M. Yant, Levi |
| author_facet | Edwards, Anne Njaci, Isaac Sarkar, Abhimanyu Jiang, Zhouqian Kaithakottil, Gemy George Moore, Christopher Cheema, Jitender Stevenson, Clare E.M. Rejzek, Martin Novák, Petr Vigouroux, Marielle Vickers, Martin Wouters, Roland H.M. Paajanen, Pirita Steuernagel, Burkhard Moore, Jonathan D. Higgins, Janet Swarbreck, David Martens, Stefan Kim, Colin Y. Weng, Jing-Ke Mundree, Sagadevan Kilian, Benjamin Kumar, Shiv Loose, Matt Yant, Levi Macas, Jiří Wang, Trevor L. Martin, Cathie Emmrich, Peter M.F. |
| author_sort | Edwards, Anne |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Grass pea ( Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin. |
| format | Journal Article |
| id | CGSpace129553 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1295532025-03-13T08:45:17Z Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus Edwards, Anne Njaci, Isaac Sarkar, Abhimanyu Jiang, Zhouqian Kaithakottil, Gemy George Moore, Christopher Cheema, Jitender Stevenson, Clare E.M. Rejzek, Martin Novák, Petr Vigouroux, Marielle Vickers, Martin Wouters, Roland H.M. Paajanen, Pirita Steuernagel, Burkhard Moore, Jonathan D. Higgins, Janet Swarbreck, David Martens, Stefan Kim, Colin Y. Weng, Jing-Ke Mundree, Sagadevan Kilian, Benjamin Kumar, Shiv Loose, Matt Yant, Levi Macas, Jiří Wang, Trevor L. Martin, Cathie Emmrich, Peter M.F. genomics biosynthesis lathyrus Grass pea ( Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the neurotoxin, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin. 2023-02-16 2023-03-10T14:38:58Z 2023-03-10T14:38:58Z Journal Article https://hdl.handle.net/10568/129553 en Open Access Springer Edwards, Anne; Njaci, Isaac; Sarkar, Abhimanyu; Jiang, Zhouqian; Kaithakottil, Gemy George; Moore, Christopher; Cheema, Jitender; Stevenson, Clare E.M.; Rejzek, Martin; Novák, Petr; Vigouroux, Marielle; Vickers, Martin; Wouters, Roland H.M.; Paajanen, Pirita; Steuernagel, Burkhard; Moore, Jonathan D.; Higgins, Janet; Swarbreck, David; Martens, Stefan; Kim, Colin Y.; Weng, Jing-Ke; Mundree, Sagadevan; Kilian, Benjamin; Kumar, Shiv; Loose, Matt; Yant, Levi; Macas, Jiří; Wang, Trevor L.; Martin, Cathie; Emmrich, Peter M.F. 2023. Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus. Nature Communications 14: |
| spellingShingle | genomics biosynthesis lathyrus Edwards, Anne Njaci, Isaac Sarkar, Abhimanyu Jiang, Zhouqian Kaithakottil, Gemy George Moore, Christopher Cheema, Jitender Stevenson, Clare E.M. Rejzek, Martin Novák, Petr Vigouroux, Marielle Vickers, Martin Wouters, Roland H.M. Paajanen, Pirita Steuernagel, Burkhard Moore, Jonathan D. Higgins, Janet Swarbreck, David Martens, Stefan Kim, Colin Y. Weng, Jing-Ke Mundree, Sagadevan Kilian, Benjamin Kumar, Shiv Loose, Matt Yant, Levi Macas, Jiří Wang, Trevor L. Martin, Cathie Emmrich, Peter M.F. Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus |
| title | Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus |
| title_full | Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus |
| title_fullStr | Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus |
| title_full_unstemmed | Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus |
| title_short | Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus |
| title_sort | genomics and biochemical analyses reveal a metabolon key to β l odap biosynthesis in lathyrus sativus |
| topic | genomics biosynthesis lathyrus |
| url | https://hdl.handle.net/10568/129553 |
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