Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis
Control and elimination of human African trypanosomiasis (HAT) can be accelerated through the use of diagnostic tests that are more accurate and easier to deploy. The goal of this work was to evaluate the immuno-reactivity of antigens and identify candidates to be considered for development of a sim...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Public Library of Science
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/129481 |
| _version_ | 1855534352368140288 |
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| author | Biéler, Sylvain Waltenberger, Harald Barrett, Michael P. McCulloch, Richard Mottram, Jeremy C. Carrington, Mark Schwaeble, Wilhelm McKerrow, James Phillips, Margaret A. Michels, Paul A. Büscher, Philippe Sanchez, Jean-Charles Bishop, Richard P. Robinson, Derrick R. Bangs, James Ferguson, Michael Nerima, Barbara Albertini, Audrey Michel, Gerd Radwandska, Magdalena Ndung’u, Joseph Mathu |
| author_browse | Albertini, Audrey Bangs, James Barrett, Michael P. Bishop, Richard P. Biéler, Sylvain Büscher, Philippe Carrington, Mark Ferguson, Michael McCulloch, Richard McKerrow, James Michel, Gerd Michels, Paul A. Mottram, Jeremy C. Ndung’u, Joseph Mathu Nerima, Barbara Phillips, Margaret A. Radwandska, Magdalena Robinson, Derrick R. Sanchez, Jean-Charles Schwaeble, Wilhelm Waltenberger, Harald |
| author_facet | Biéler, Sylvain Waltenberger, Harald Barrett, Michael P. McCulloch, Richard Mottram, Jeremy C. Carrington, Mark Schwaeble, Wilhelm McKerrow, James Phillips, Margaret A. Michels, Paul A. Büscher, Philippe Sanchez, Jean-Charles Bishop, Richard P. Robinson, Derrick R. Bangs, James Ferguson, Michael Nerima, Barbara Albertini, Audrey Michel, Gerd Radwandska, Magdalena Ndung’u, Joseph Mathu |
| author_sort | Biéler, Sylvain |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Control and elimination of human African trypanosomiasis (HAT) can be accelerated through the use of diagnostic tests that are more accurate and easier to deploy. The goal of this work was to evaluate the immuno-reactivity of antigens and identify candidates to be considered for development of a simple serological test for the detection of Trypanosoma brucei gambiense or T. b. rhodesiense infections, ideally both.
The reactivity of 35 antigens was independently evaluated by slot blot and ELISA against sera from both T. b. gambiense and T. b. rhodesiense infected patients and controls. The antigens that were most reactive by both tests to T. b. gambiense sera were the membrane proteins VSG LiTat 1.3, VSG LiTat 1.5 and ISG64. Reactivity to T. b. rhodesiense sera was highest with VSG LiTat 1.3, VSG LiTat 1.5 and SRA, although much lower than with T. b. gambiense samples. The reactivity of all possible combinations of antigens was also calculated. When the slot blot results of 2 antigens were paired, a VSG LiTat 1.3- ISG75 combination performed best on T. b. gambiense sera, while a VSG LiTat 1.3-VSG LiTat 1.5 combination was the most reactive using ELISA. A combination of SRA and either VSG LiTat 1.3 or VSG LiTat 1.5 had the highest reactivity on T. b. rhodesiense sera according to slot blot, while in ELISA, pairing SRA with either GM6 or VSG LiTat 1.3 yielded the best results.
This study identified antigens that were highly reactive to T. b. gambiense sera, which could be considered for developing a serological test for gambiense HAT, either individually or in combination. Antigens with potential for inclusion in a test for T. b. rhodesiense HAT were also identified, but because their reactivity was comparatively lower, a search for additional antigens would be required before developing a test for this form of the disease. |
| format | Journal Article |
| id | CGSpace129481 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1294812025-12-08T09:54:28Z Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis Biéler, Sylvain Waltenberger, Harald Barrett, Michael P. McCulloch, Richard Mottram, Jeremy C. Carrington, Mark Schwaeble, Wilhelm McKerrow, James Phillips, Margaret A. Michels, Paul A. Büscher, Philippe Sanchez, Jean-Charles Bishop, Richard P. Robinson, Derrick R. Bangs, James Ferguson, Michael Nerima, Barbara Albertini, Audrey Michel, Gerd Radwandska, Magdalena Ndung’u, Joseph Mathu evaluation trypanosomiasis development antigens african trypanosomiasis Control and elimination of human African trypanosomiasis (HAT) can be accelerated through the use of diagnostic tests that are more accurate and easier to deploy. The goal of this work was to evaluate the immuno-reactivity of antigens and identify candidates to be considered for development of a simple serological test for the detection of Trypanosoma brucei gambiense or T. b. rhodesiense infections, ideally both. The reactivity of 35 antigens was independently evaluated by slot blot and ELISA against sera from both T. b. gambiense and T. b. rhodesiense infected patients and controls. The antigens that were most reactive by both tests to T. b. gambiense sera were the membrane proteins VSG LiTat 1.3, VSG LiTat 1.5 and ISG64. Reactivity to T. b. rhodesiense sera was highest with VSG LiTat 1.3, VSG LiTat 1.5 and SRA, although much lower than with T. b. gambiense samples. The reactivity of all possible combinations of antigens was also calculated. When the slot blot results of 2 antigens were paired, a VSG LiTat 1.3- ISG75 combination performed best on T. b. gambiense sera, while a VSG LiTat 1.3-VSG LiTat 1.5 combination was the most reactive using ELISA. A combination of SRA and either VSG LiTat 1.3 or VSG LiTat 1.5 had the highest reactivity on T. b. rhodesiense sera according to slot blot, while in ELISA, pairing SRA with either GM6 or VSG LiTat 1.3 yielded the best results. This study identified antigens that were highly reactive to T. b. gambiense sera, which could be considered for developing a serological test for gambiense HAT, either individually or in combination. Antigens with potential for inclusion in a test for T. b. rhodesiense HAT were also identified, but because their reactivity was comparatively lower, a search for additional antigens would be required before developing a test for this form of the disease. 2016-12-09 2023-03-10T14:36:27Z 2023-03-10T14:36:27Z Journal Article https://hdl.handle.net/10568/129481 en Open Access Public Library of Science Biéler, Sylvain; Waltenberger, Harald; Barrett, Michael P.; McCulloch, Richard; Mottram, Jeremy C.; Carrington, Mark; Schwaeble, Wilhelm; McKerrow, James; Phillips, Margaret A.; Michels, Paul A.; Büscher, Philippe; Sanchez, Jean-Charles; Bishop, Richard P.; Robinson, Derrick R.; Bangs, James; Ferguson, Michael; Nerima, Barbara; Albertini, Audrey; Michel, Gerd; Radwandska, Magdalena; Ndung'u, Joseph Mathu. 2016. Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis. PLOS ONE 11: e0168074 |
| spellingShingle | evaluation trypanosomiasis development antigens african trypanosomiasis Biéler, Sylvain Waltenberger, Harald Barrett, Michael P. McCulloch, Richard Mottram, Jeremy C. Carrington, Mark Schwaeble, Wilhelm McKerrow, James Phillips, Margaret A. Michels, Paul A. Büscher, Philippe Sanchez, Jean-Charles Bishop, Richard P. Robinson, Derrick R. Bangs, James Ferguson, Michael Nerima, Barbara Albertini, Audrey Michel, Gerd Radwandska, Magdalena Ndung’u, Joseph Mathu Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis |
| title | Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis |
| title_full | Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis |
| title_fullStr | Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis |
| title_full_unstemmed | Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis |
| title_short | Evaluation of Antigens for Development of a Serological Test for Human African Trypanosomiasis |
| title_sort | evaluation of antigens for development of a serological test for human african trypanosomiasis |
| topic | evaluation trypanosomiasis development antigens african trypanosomiasis |
| url | https://hdl.handle.net/10568/129481 |
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