Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models
Respiratory infections caused by mycoplasma species in ruminants lead to considerable economic losses. Two important ruminant pathogens are Mycoplasma mycoides subsp. Mycoides (Mmm), the aetiological agent of contagious bovine pleuropneumonia and Mycoplasma mycoides subsp. capri (Mmc), which causes...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2019
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| Online Access: | https://hdl.handle.net/10568/129439 |
| _version_ | 1855524468289437696 |
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| author | Weldearegay, Yenehiwot Müller, Sandy Hänske, Jana Schulze, Anja Kostka, Aline Rüger, Nancy Hewicker-Trautwein, Marion Brehm, Ralph Valentin-Weigand, Peter Kammerer, Robert Jores, Joerg Meens, Jochen |
| author_browse | Brehm, Ralph Hewicker-Trautwein, Marion Hänske, Jana Jores, Joerg Kammerer, Robert Kostka, Aline Meens, Jochen Müller, Sandy Rüger, Nancy Schulze, Anja Valentin-Weigand, Peter Weldearegay, Yenehiwot |
| author_facet | Weldearegay, Yenehiwot Müller, Sandy Hänske, Jana Schulze, Anja Kostka, Aline Rüger, Nancy Hewicker-Trautwein, Marion Brehm, Ralph Valentin-Weigand, Peter Kammerer, Robert Jores, Joerg Meens, Jochen |
| author_sort | Weldearegay, Yenehiwot |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Respiratory infections caused by mycoplasma species in ruminants lead to considerable economic losses. Two important ruminant pathogens are Mycoplasma mycoides subsp. Mycoides (Mmm), the aetiological agent of contagious bovine pleuropneumonia and Mycoplasma mycoides subsp. capri (Mmc), which causes pneumonia, mastitis, arthritis, keratitis, and septicemia in goats. We established precision cut lung slices (PCLS) infection model for Mmm and Mmc to study host-pathogen interactions. We monitored infection over time using immunohistological analysis and electron microscopy. Moreover, infection burden was monitored by plating and quantitative real-time PCR. Results were compared with lungs from experimentally infected goats and cattle. Lungs from healthy goats and cattle were also included as controls. PCLS remained viable for up to two weeks. Both subspecies adhered to ciliated cells. However, the titer of Mmm in caprine PCLS decreased over time, indicating species specificity of Mmm. Mmc showed higher tropism to sub-bronchiolar tissue in caprine PCLS, which increased in a time-dependent manner. Moreover, Mmc was abundantly observed on pulmonary endothelial cells, indicating partially, how it causes systemic disease. Tissue destruction upon prolonged infection of slices was comparable to the in vivo samples. Therefore, PCLS represents a novel ex vivo model to study host-pathogen interaction in livestock mycoplasma. |
| format | Journal Article |
| id | CGSpace129439 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1294392025-12-08T10:11:39Z Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models Weldearegay, Yenehiwot Müller, Sandy Hänske, Jana Schulze, Anja Kostka, Aline Rüger, Nancy Hewicker-Trautwein, Marion Brehm, Ralph Valentin-Weigand, Peter Kammerer, Robert Jores, Joerg Meens, Jochen models pathogen mycoplasma bovine mycoplasma mycoides interactions Respiratory infections caused by mycoplasma species in ruminants lead to considerable economic losses. Two important ruminant pathogens are Mycoplasma mycoides subsp. Mycoides (Mmm), the aetiological agent of contagious bovine pleuropneumonia and Mycoplasma mycoides subsp. capri (Mmc), which causes pneumonia, mastitis, arthritis, keratitis, and septicemia in goats. We established precision cut lung slices (PCLS) infection model for Mmm and Mmc to study host-pathogen interactions. We monitored infection over time using immunohistological analysis and electron microscopy. Moreover, infection burden was monitored by plating and quantitative real-time PCR. Results were compared with lungs from experimentally infected goats and cattle. Lungs from healthy goats and cattle were also included as controls. PCLS remained viable for up to two weeks. Both subspecies adhered to ciliated cells. However, the titer of Mmm in caprine PCLS decreased over time, indicating species specificity of Mmm. Mmc showed higher tropism to sub-bronchiolar tissue in caprine PCLS, which increased in a time-dependent manner. Moreover, Mmc was abundantly observed on pulmonary endothelial cells, indicating partially, how it causes systemic disease. Tissue destruction upon prolonged infection of slices was comparable to the in vivo samples. Therefore, PCLS represents a novel ex vivo model to study host-pathogen interaction in livestock mycoplasma. 2019-06-20 2023-03-10T14:35:22Z 2023-03-10T14:35:22Z Journal Article https://hdl.handle.net/10568/129439 en Open Access MDPI Weldearegay, Yenehiwot; Müller, Sandy; Hänske, Jana; Schulze, Anja; Kostka, Aline; Rüger, Nancy; Hewicker-Trautwein, Marion; Brehm, Ralph; Valentin-Weigand, Peter; Kammerer, Robert; Jores, Joerg; Meens, Jochen. 2019. Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices PCLS Models. Pathogens 8: 82 |
| spellingShingle | models pathogen mycoplasma bovine mycoplasma mycoides interactions Weldearegay, Yenehiwot Müller, Sandy Hänske, Jana Schulze, Anja Kostka, Aline Rüger, Nancy Hewicker-Trautwein, Marion Brehm, Ralph Valentin-Weigand, Peter Kammerer, Robert Jores, Joerg Meens, Jochen Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title | Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_full | Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_fullStr | Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_full_unstemmed | Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_short | Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models |
| title_sort | host pathogen interactions of mycoplasma mycoides in caprine and bovine precision cut lung slices pcls models |
| topic | models pathogen mycoplasma bovine mycoplasma mycoides interactions |
| url | https://hdl.handle.net/10568/129439 |
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