Mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis
Tuberculosis (TB) is a major cause of mortality and suffering worldwide, with over 95% of TB deaths occurring in low- and middle-income countries. In recent years, molecular typing methods have been widely used in epidemiological studies to aid the control of TB, but this usage has not been the case...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2013
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| Online Access: | https://hdl.handle.net/10568/129399 |
| _version_ | 1855537301370699776 |
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| author | Assam, Jean Paul Assam Beng, Véronique Penlap Cho-Ngwa, Fidelis Toukam, Michel Ngoh, Ane-Anyangwe Irene Kitavi, Mercy Nzuki, Inosters Nyonka, Juliette N Tata, Emilienne Tedom, Jean Claude Skilton, Robert A. Pellé, Roger Titanji, Vincent Pryde Kehdingha |
| author_browse | Assam, Jean Paul Assam Beng, Véronique Penlap Cho-Ngwa, Fidelis Kitavi, Mercy Ngoh, Ane-Anyangwe Irene Nyonka, Juliette N Nzuki, Inosters Pellé, Roger Skilton, Robert A. Tata, Emilienne Tedom, Jean Claude Titanji, Vincent Pryde Kehdingha Toukam, Michel |
| author_facet | Assam, Jean Paul Assam Beng, Véronique Penlap Cho-Ngwa, Fidelis Toukam, Michel Ngoh, Ane-Anyangwe Irene Kitavi, Mercy Nzuki, Inosters Nyonka, Juliette N Tata, Emilienne Tedom, Jean Claude Skilton, Robert A. Pellé, Roger Titanji, Vincent Pryde Kehdingha |
| author_sort | Assam, Jean Paul Assam |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Tuberculosis (TB) is a major cause of mortality and suffering worldwide, with over 95% of TB deaths occurring in low- and middle-income countries. In recent years, molecular typing methods have been widely used in epidemiological studies to aid the control of TB, but this usage has not been the case with many African countries, including Cameroon. The aims of the present investigation were to identify and evaluate the diversity of the Mycobacterium tuberculosis complex (MTBC) isolates circulating in two ecological zones of Cameroon, seven years after the last studies in the West Region, and after the re-organization of the National TB Control Program (NTBCP). These were expected to shed light also on the transmission of TB in the country. The study was conducted from February to July 2009. During this period, 169 patients with symptomatic disease and with sputum cultures that were positive for MTBC were randomly selected for the study from amongst 964 suspected patients in the savannah mosaic zone (West and North West regions) and the tropical rainforest zone (Central region). After culture and diagnosis, DNA was extracted from each of the MTBC isolates and transported to the BecA-ILRI Hub in Nairobi, Kenya for molecular analysis.Genetic characterization was done by mycobacterial interspersed repetitive unit–variable number tandem repeat typing (MIRU-VNTR) and Spoligotyping.Molecular analysis showed that all TB cases reported in this study were caused by infections with Mycobacterium tuberculosis (98.8%) and Mycobacterium africanum (M. africanum) (1.2%) respectively. We did not detect any M. bovis. Comparative analyses using spoligotyping revealed that the majority of isolates belong to major clades of M. tuberculosis: Haarlem (7.6%), Latin American-Mediterranean (34.4%) and T clade (26.7%); the remaining isolates (31.3%) where distributed among the minor clades. The predominant group of isolates (34.4%) corresponded to spoligotype 61, previously described as the “Cameroon family. Further analysis based on MIRU-VNTR profiles had greater resolving power than spoligotyping and defined additional genotypes in the same spoligotype cluster.The molecular characterization of MTBC strains from humans in two ecological regions of Cameroon has shown that M. tuberculosis sensu stricto is the predominant agent of TB cases in the zones. Three decades ago, TB was reported to be caused by M. africanum in 56.0% of cases. The present findings are consistent with a major shift in the prevalence of M. tuberculosis in Cameroon. |
| format | Journal Article |
| id | CGSpace129399 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1293992025-01-27T15:00:52Z Mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis Assam, Jean Paul Assam Beng, Véronique Penlap Cho-Ngwa, Fidelis Toukam, Michel Ngoh, Ane-Anyangwe Irene Kitavi, Mercy Nzuki, Inosters Nyonka, Juliette N Tata, Emilienne Tedom, Jean Claude Skilton, Robert A. Pellé, Roger Titanji, Vincent Pryde Kehdingha analysis cameroon ecological zones tuberculosis mycobacterium Tuberculosis (TB) is a major cause of mortality and suffering worldwide, with over 95% of TB deaths occurring in low- and middle-income countries. In recent years, molecular typing methods have been widely used in epidemiological studies to aid the control of TB, but this usage has not been the case with many African countries, including Cameroon. The aims of the present investigation were to identify and evaluate the diversity of the Mycobacterium tuberculosis complex (MTBC) isolates circulating in two ecological zones of Cameroon, seven years after the last studies in the West Region, and after the re-organization of the National TB Control Program (NTBCP). These were expected to shed light also on the transmission of TB in the country. The study was conducted from February to July 2009. During this period, 169 patients with symptomatic disease and with sputum cultures that were positive for MTBC were randomly selected for the study from amongst 964 suspected patients in the savannah mosaic zone (West and North West regions) and the tropical rainforest zone (Central region). After culture and diagnosis, DNA was extracted from each of the MTBC isolates and transported to the BecA-ILRI Hub in Nairobi, Kenya for molecular analysis.Genetic characterization was done by mycobacterial interspersed repetitive unit–variable number tandem repeat typing (MIRU-VNTR) and Spoligotyping.Molecular analysis showed that all TB cases reported in this study were caused by infections with Mycobacterium tuberculosis (98.8%) and Mycobacterium africanum (M. africanum) (1.2%) respectively. We did not detect any M. bovis. Comparative analyses using spoligotyping revealed that the majority of isolates belong to major clades of M. tuberculosis: Haarlem (7.6%), Latin American-Mediterranean (34.4%) and T clade (26.7%); the remaining isolates (31.3%) where distributed among the minor clades. The predominant group of isolates (34.4%) corresponded to spoligotype 61, previously described as the “Cameroon family. Further analysis based on MIRU-VNTR profiles had greater resolving power than spoligotyping and defined additional genotypes in the same spoligotype cluster.The molecular characterization of MTBC strains from humans in two ecological regions of Cameroon has shown that M. tuberculosis sensu stricto is the predominant agent of TB cases in the zones. Three decades ago, TB was reported to be caused by M. africanum in 56.0% of cases. The present findings are consistent with a major shift in the prevalence of M. tuberculosis in Cameroon. 2013-12 2023-03-10T14:34:27Z 2023-03-10T14:34:27Z Journal Article https://hdl.handle.net/10568/129399 en Open Access Springer Assam, Jean Paul Assam; Beng, Véronique Penlap; Cho-Ngwa, Fidelis; Toukam, Michel; Ngoh, Ane-Anyangwe Irene; Kitavi, Mercy; Nzuki, Inosters; Nyonka, Juliette N; Tata, Emilienne; Tedom, Jean Claude; Skilton, Robert A.; Pellé, Roger; Titanji, Vincent Pryde Kehdingha. 2013. Mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis. BMC Infectious Diseases 13: |
| spellingShingle | analysis cameroon ecological zones tuberculosis mycobacterium Assam, Jean Paul Assam Beng, Véronique Penlap Cho-Ngwa, Fidelis Toukam, Michel Ngoh, Ane-Anyangwe Irene Kitavi, Mercy Nzuki, Inosters Nyonka, Juliette N Tata, Emilienne Tedom, Jean Claude Skilton, Robert A. Pellé, Roger Titanji, Vincent Pryde Kehdingha Mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis |
| title | Mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis |
| title_full | Mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis |
| title_fullStr | Mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis |
| title_full_unstemmed | Mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis |
| title_short | Mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of Cameroon, as shown by genetic analysis |
| title_sort | mycobacterium tuberculosisis the causative agent of tuberculosis in the southern ecological zones of cameroon as shown by genetic analysis |
| topic | analysis cameroon ecological zones tuberculosis mycobacterium |
| url | https://hdl.handle.net/10568/129399 |
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