Distribution and genetic diversity of Tomato spotted wilt virus following an incursion into Kenya
Tomato spotted wilt virus (TSWV) affects the production of many horticultural crops worldwide. It was first reported from Kenya in 1999. The occurrence, distribution and genetic diversity of TSWV were evaluated in four tomato production areas in Kenya a decade after this incursion. The awareness of...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2015
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/129361 |
| _version_ | 1855522398563991552 |
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| author | Macharia, I. Backhouse, D. Ateka, Elijah Miinda Wu, S.-B. Harvey, Jagger J.W. Njahira, Moses N. Skilton, Robert A. |
| author_browse | Ateka, Elijah Miinda Backhouse, D. Harvey, Jagger J.W. Macharia, I. Njahira, Moses N. Skilton, Robert A. Wu, S.-B. |
| author_facet | Macharia, I. Backhouse, D. Ateka, Elijah Miinda Wu, S.-B. Harvey, Jagger J.W. Njahira, Moses N. Skilton, Robert A. |
| author_sort | Macharia, I. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Tomato spotted wilt virus (TSWV) affects the production of many horticultural crops worldwide. It was first reported from Kenya in 1999. The occurrence, distribution and genetic diversity of TSWV were evaluated in four tomato production areas in Kenya a decade after this incursion. The awareness of TSWV and its vectors among farmers was assessed through a questionnaire while plant samples including tomato leaves and fruit were collected from diseased and non‐diseased plants. The samples were assayed for TSWV using ELISA and reverse transcriptase‐polymerase chain reaction, and the resulting positive samples were sequenced. There was less awareness of the virus, its vectors and alternate hosts among farmers, despite the occurrence of the disease for over a decade. A total of 89 of 408 tomato samples tested positive for TSWV. Most of the positive samples came from Nakuru, where the virus was first detected, with only a single positive sample being obtained from Loitokitok and none from Bungoma or Kirinyaga. A phylogenetic analysis based on partial nucleocapsid (N) protein gene sequences suggested that the Kenyan isolates formed a single subgroup nested within a cluster of isolates that came predominantly from Europe. This indicated a single introduction that had undergone limited diversification. The study revealed that the disease has persisted in the area to which it was first introduced but has had very limited dispersal to other areas. |
| format | Journal Article |
| id | CGSpace129361 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1293612025-12-08T09:54:28Z Distribution and genetic diversity of Tomato spotted wilt virus following an incursion into Kenya Macharia, I. Backhouse, D. Ateka, Elijah Miinda Wu, S.-B. Harvey, Jagger J.W. Njahira, Moses N. Skilton, Robert A. distribution virus kenya wilt tomato spotted wilt virus tomatoes Tomato spotted wilt virus (TSWV) affects the production of many horticultural crops worldwide. It was first reported from Kenya in 1999. The occurrence, distribution and genetic diversity of TSWV were evaluated in four tomato production areas in Kenya a decade after this incursion. The awareness of TSWV and its vectors among farmers was assessed through a questionnaire while plant samples including tomato leaves and fruit were collected from diseased and non‐diseased plants. The samples were assayed for TSWV using ELISA and reverse transcriptase‐polymerase chain reaction, and the resulting positive samples were sequenced. There was less awareness of the virus, its vectors and alternate hosts among farmers, despite the occurrence of the disease for over a decade. A total of 89 of 408 tomato samples tested positive for TSWV. Most of the positive samples came from Nakuru, where the virus was first detected, with only a single positive sample being obtained from Loitokitok and none from Bungoma or Kirinyaga. A phylogenetic analysis based on partial nucleocapsid (N) protein gene sequences suggested that the Kenyan isolates formed a single subgroup nested within a cluster of isolates that came predominantly from Europe. This indicated a single introduction that had undergone limited diversification. The study revealed that the disease has persisted in the area to which it was first introduced but has had very limited dispersal to other areas. 2015-05 2023-03-10T14:33:45Z 2023-03-10T14:33:45Z Journal Article https://hdl.handle.net/10568/129361 en Limited Access Wiley Macharia, I.; Backhouse, D.; Ateka, Elijah Miinda; Wu, S.B.; Harvey, Jagger J.W.; Njahira, Moses N.; Skilton, Robert A. 2015. Distribution and genetic diversity of Tomato spotted wilt virus following an incursion into Kenya. Annals of Applied Biology 166: 520-529 |
| spellingShingle | distribution virus kenya wilt tomato spotted wilt virus tomatoes Macharia, I. Backhouse, D. Ateka, Elijah Miinda Wu, S.-B. Harvey, Jagger J.W. Njahira, Moses N. Skilton, Robert A. Distribution and genetic diversity of Tomato spotted wilt virus following an incursion into Kenya |
| title | Distribution and genetic diversity of Tomato spotted wilt virus following an incursion into Kenya |
| title_full | Distribution and genetic diversity of Tomato spotted wilt virus following an incursion into Kenya |
| title_fullStr | Distribution and genetic diversity of Tomato spotted wilt virus following an incursion into Kenya |
| title_full_unstemmed | Distribution and genetic diversity of Tomato spotted wilt virus following an incursion into Kenya |
| title_short | Distribution and genetic diversity of Tomato spotted wilt virus following an incursion into Kenya |
| title_sort | distribution and genetic diversity of tomato spotted wilt virus following an incursion into kenya |
| topic | distribution virus kenya wilt tomato spotted wilt virus tomatoes |
| url | https://hdl.handle.net/10568/129361 |
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