Metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen
Rumen microbiota play a key role in the digestion and utilization of plant materials by the ruminant species, which have important implications for greenhouse gas emission. Yet, little is known about the key taxa and potential gene functions involved in the digestion process. Here, we performed a ge...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2021
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| Acceso en línea: | https://hdl.handle.net/10568/129314 |
| _version_ | 1855536535343988736 |
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| author | Gharechahi, Javad Vahidi, Mohammad Farhad Bahram, Mohammad Han Jianlin Ding, Xue-Zhi Salekdeh, Ghasem Hosseini |
| author_browse | Bahram, Mohammad Ding, Xue-Zhi Gharechahi, Javad Han Jianlin Salekdeh, Ghasem Hosseini Vahidi, Mohammad Farhad |
| author_facet | Gharechahi, Javad Vahidi, Mohammad Farhad Bahram, Mohammad Han Jianlin Ding, Xue-Zhi Salekdeh, Ghasem Hosseini |
| author_sort | Gharechahi, Javad |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rumen microbiota play a key role in the digestion and utilization of plant materials by the ruminant species, which have important implications for greenhouse gas emission. Yet, little is known about the key taxa and potential gene functions involved in the digestion process. Here, we performed a genome-centric analysis of rumen microbiota attached to six different lignocellulosic biomasses in rumen-fistulated cattle. Our metagenome sequencing provided novel genomic insights into functional potential of 523 uncultured bacteria and 15 mostly uncultured archaea in the rumen. The assembled genomes belonged mainly to Bacteroidota, Firmicutes, Verrucomicrobiota, and Fibrobacterota and were enriched for genes related to the degradation of lignocellulosic polymers and the fermentation of degraded products into short chain volatile fatty acids. We also found a shift from copiotrophic to oligotrophic taxa during the course of rumen fermentation, potentially important for the digestion of recalcitrant lignocellulosic substrates in the physiochemically complex and varying environment of the rumen. Differential colonization of forages (the incubated lignocellulosic materials) by rumen microbiota suggests that taxonomic and metabolic diversification is an evolutionary adaptation to diverse lignocellulosic substrates constituting a major component of the cattle’s diet. Our data also provide novel insights into the key role of unique microbial diversity and associated gene functions in the degradation of recalcitrant lignocellulosic materials in the rumen. |
| format | Journal Article |
| id | CGSpace129314 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1293142024-11-15T08:52:58Z Metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen Gharechahi, Javad Vahidi, Mohammad Farhad Bahram, Mohammad Han Jianlin Ding, Xue-Zhi Salekdeh, Ghasem Hosseini cattle analysis rumen Rumen microbiota play a key role in the digestion and utilization of plant materials by the ruminant species, which have important implications for greenhouse gas emission. Yet, little is known about the key taxa and potential gene functions involved in the digestion process. Here, we performed a genome-centric analysis of rumen microbiota attached to six different lignocellulosic biomasses in rumen-fistulated cattle. Our metagenome sequencing provided novel genomic insights into functional potential of 523 uncultured bacteria and 15 mostly uncultured archaea in the rumen. The assembled genomes belonged mainly to Bacteroidota, Firmicutes, Verrucomicrobiota, and Fibrobacterota and were enriched for genes related to the degradation of lignocellulosic polymers and the fermentation of degraded products into short chain volatile fatty acids. We also found a shift from copiotrophic to oligotrophic taxa during the course of rumen fermentation, potentially important for the digestion of recalcitrant lignocellulosic substrates in the physiochemically complex and varying environment of the rumen. Differential colonization of forages (the incubated lignocellulosic materials) by rumen microbiota suggests that taxonomic and metabolic diversification is an evolutionary adaptation to diverse lignocellulosic substrates constituting a major component of the cattle’s diet. Our data also provide novel insights into the key role of unique microbial diversity and associated gene functions in the degradation of recalcitrant lignocellulosic materials in the rumen. 2021-04 2023-03-10T14:33:10Z 2023-03-10T14:33:10Z Journal Article https://hdl.handle.net/10568/129314 en Open Access Springer Gharechahi, Javad; Vahidi, Mohammad Farhad; Bahram, Mohammad; Han Jianlin; Ding, Xue-Zhi; Salekdeh, Ghasem Hosseini. 2021. Metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen. The ISME Journal 15: 1108-1120 |
| spellingShingle | cattle analysis rumen Gharechahi, Javad Vahidi, Mohammad Farhad Bahram, Mohammad Han Jianlin Ding, Xue-Zhi Salekdeh, Ghasem Hosseini Metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen |
| title | Metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen |
| title_full | Metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen |
| title_fullStr | Metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen |
| title_full_unstemmed | Metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen |
| title_short | Metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen |
| title_sort | metagenomic analysis reveals a dynamic microbiome with diversified adaptive functions to utilize high lignocellulosic forages in the cattle rumen |
| topic | cattle analysis rumen |
| url | https://hdl.handle.net/10568/129314 |
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