Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting
Genetic analysis of Trypanosoma spp. depends on the detection of variation between strains. We have used the amplified fragment length polymorphism (AFLP) technique to develop a convenient and reliable method for genetic characterization of Trypanosome (sub)species. AFLP accesses multiple independen...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
2002
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/129311 |
| _version_ | 1855523388664053760 |
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| author | Agbo, E.E.C. Majiwa, Phelix A.O. Claassen, H.J.H.M. Te Pas, M.F.W. |
| author_browse | Agbo, E.E.C. Claassen, H.J.H.M. Majiwa, Phelix A.O. Te Pas, M.F.W. |
| author_facet | Agbo, E.E.C. Majiwa, Phelix A.O. Claassen, H.J.H.M. Te Pas, M.F.W. |
| author_sort | Agbo, E.E.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Genetic analysis of Trypanosoma spp. depends on the detection of variation between strains. We have used the amplified fragment length polymorphism (AFLP) technique to develop a convenient and reliable method for genetic characterization of Trypanosome (sub)species. AFLP accesses multiple independent sites within the genome and would allow a better definition of the relatedness of different Trypanosome (sub)species. Nine isolates (3 from each T. brucei subspecies) were tested with 40 AFLP primer combinations to identify the most appropriate pairs of restriction endonucleases and selective primers. Primers based on the recognition sequences of EcoRI and BglII were chosen and used to analyse 31 T. brucei isolates. Similarity levels calculated with the Pearson correlation coefficient ranged from 15 to 98%, and clusters were determined using the unweighted pair-group method using arithmetic averages (UPGMA). At the intraspecific level, AFLP fingerprints were grouped by numerical analysis in 2 main clusters, allowing a clear separation of T. b. gambiense (cluster I) from T. b. brucei and T. b. rhodesiense isolates (cluster II). Interspecies evaluation of this customized approach produced heterogeneous AFLP patterns, with unique genetic markers, except for T. evansi and T. equiperdum, which showed identical patterns and clustered together. |
| format | Journal Article |
| id | CGSpace129311 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace1293112025-12-08T09:54:28Z Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting Agbo, E.E.C. Majiwa, Phelix A.O. Claassen, H.J.H.M. Te Pas, M.F.W. trypanosoma brucei aflp subspecies Genetic analysis of Trypanosoma spp. depends on the detection of variation between strains. We have used the amplified fragment length polymorphism (AFLP) technique to develop a convenient and reliable method for genetic characterization of Trypanosome (sub)species. AFLP accesses multiple independent sites within the genome and would allow a better definition of the relatedness of different Trypanosome (sub)species. Nine isolates (3 from each T. brucei subspecies) were tested with 40 AFLP primer combinations to identify the most appropriate pairs of restriction endonucleases and selective primers. Primers based on the recognition sequences of EcoRI and BglII were chosen and used to analyse 31 T. brucei isolates. Similarity levels calculated with the Pearson correlation coefficient ranged from 15 to 98%, and clusters were determined using the unweighted pair-group method using arithmetic averages (UPGMA). At the intraspecific level, AFLP fingerprints were grouped by numerical analysis in 2 main clusters, allowing a clear separation of T. b. gambiense (cluster I) from T. b. brucei and T. b. rhodesiense isolates (cluster II). Interspecies evaluation of this customized approach produced heterogeneous AFLP patterns, with unique genetic markers, except for T. evansi and T. equiperdum, which showed identical patterns and clustered together. 2002-04 2023-03-10T14:33:09Z 2023-03-10T14:33:09Z Journal Article https://hdl.handle.net/10568/129311 en Limited Access Cambridge University Press Agbo, E.E.C.; Majiwa, P.A.O.; Claassen, H.J.H.M.; Te Pas, M.F.W. 2002. Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting. Parasitology 124: 349-358 |
| spellingShingle | trypanosoma brucei aflp subspecies Agbo, E.E.C. Majiwa, Phelix A.O. Claassen, H.J.H.M. Te Pas, M.F.W. Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting |
| title | Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting |
| title_full | Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting |
| title_fullStr | Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting |
| title_full_unstemmed | Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting |
| title_short | Molecular variation of Trypanosoma brucei subspecies as revealed by AFLP fingerprinting |
| title_sort | molecular variation of trypanosoma brucei subspecies as revealed by aflp fingerprinting |
| topic | trypanosoma brucei aflp subspecies |
| url | https://hdl.handle.net/10568/129311 |
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