Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens
Subunit viral vaccines are typically not as efficient as live attenuated or inactivated vaccines at inducing protective immune responses. This paper describes an alternative ‘biomimetic’ technology; whereby viral antigens were formulated around a polymeric shell in a rationally arranged fashion with...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2017
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| Online Access: | https://hdl.handle.net/10568/129303 |
| _version_ | 1855518395964850176 |
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| author | Riitho, Victor Walters, Adam A. Somavarapu, Satyanarayana Lamp, Benjamin Rümenapf, Till Krey, Thomas Rey, Felix A. Oviedo-Orta, Ernesto Stewart, Graham R. Locker, Nicolas Steinbach, Falko Graham, Simon P. |
| author_browse | Graham, Simon P. Krey, Thomas Lamp, Benjamin Locker, Nicolas Oviedo-Orta, Ernesto Rey, Felix A. Riitho, Victor Rümenapf, Till Somavarapu, Satyanarayana Steinbach, Falko Stewart, Graham R. Walters, Adam A. |
| author_facet | Riitho, Victor Walters, Adam A. Somavarapu, Satyanarayana Lamp, Benjamin Rümenapf, Till Krey, Thomas Rey, Felix A. Oviedo-Orta, Ernesto Stewart, Graham R. Locker, Nicolas Steinbach, Falko Graham, Simon P. |
| author_sort | Riitho, Victor |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Subunit viral vaccines are typically not as efficient as live attenuated or inactivated vaccines at inducing protective immune responses. This paper describes an alternative ‘biomimetic’ technology; whereby viral antigens were formulated around a polymeric shell in a rationally arranged fashion with a surface glycoprotein coated on to the surface and non-structural antigen and adjuvant encapsulated. We evaluated this model using BVDV E2 and NS3 proteins formulated in poly-(D, L-lactic-co-glycolic acid) (PLGA) nanoparticles adjuvanted with polyinosinic:polycytidylic acid (poly(I:C) as an adjuvant (Vaccine-NP). This Vaccine-NP was compared to ovalbumin and poly(I:C) formulated in a similar manner (Control-NP) and a commercial adjuvanted inactivated BVDV vaccine (IAV), all inoculated subcutaneously and boosted prior to BVDV-1 challenge. Significant virus-neutralizing activity, and E2 and NS3 specific antibodies were observed in both Vaccine-NP and IAV groups following the booster immunisation. IFN-γ responses were observed in ex vivo PBMC stimulated with E2 and NS3 proteins in both vaccinated groups. We observed that the protection afforded by the particulate vaccine was comparable to the licenced IAV formulation. In conclusion, the biomimetic particulates showed a promising immunogenicity and efficacy profile that may be improved by virtue of being a customisable mode of delivery. |
| format | Journal Article |
| id | CGSpace129303 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1293032025-12-08T09:54:28Z Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens Riitho, Victor Walters, Adam A. Somavarapu, Satyanarayana Lamp, Benjamin Rümenapf, Till Krey, Thomas Rey, Felix A. Oviedo-Orta, Ernesto Stewart, Graham R. Locker, Nicolas Steinbach, Falko Graham, Simon P. evaluation antigens design formulation Subunit viral vaccines are typically not as efficient as live attenuated or inactivated vaccines at inducing protective immune responses. This paper describes an alternative ‘biomimetic’ technology; whereby viral antigens were formulated around a polymeric shell in a rationally arranged fashion with a surface glycoprotein coated on to the surface and non-structural antigen and adjuvant encapsulated. We evaluated this model using BVDV E2 and NS3 proteins formulated in poly-(D, L-lactic-co-glycolic acid) (PLGA) nanoparticles adjuvanted with polyinosinic:polycytidylic acid (poly(I:C) as an adjuvant (Vaccine-NP). This Vaccine-NP was compared to ovalbumin and poly(I:C) formulated in a similar manner (Control-NP) and a commercial adjuvanted inactivated BVDV vaccine (IAV), all inoculated subcutaneously and boosted prior to BVDV-1 challenge. Significant virus-neutralizing activity, and E2 and NS3 specific antibodies were observed in both Vaccine-NP and IAV groups following the booster immunisation. IFN-γ responses were observed in ex vivo PBMC stimulated with E2 and NS3 proteins in both vaccinated groups. We observed that the protection afforded by the particulate vaccine was comparable to the licenced IAV formulation. In conclusion, the biomimetic particulates showed a promising immunogenicity and efficacy profile that may be improved by virtue of being a customisable mode of delivery. 2017-10-23 2023-03-10T14:33:05Z 2023-03-10T14:33:05Z Journal Article https://hdl.handle.net/10568/129303 en Open Access Springer Riitho, Victor; Walters, Adam A.; Somavarapu, Satyanarayana; Lamp, Benjamin; Rümenapf, Till; Krey, Thomas; Rey, Felix A.; Oviedo-Orta, Ernesto; Stewart, Graham R.; Locker, Nicolas; Steinbach, Falko; Graham, Simon P. 2017. Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens. Scientific Reports 7: |
| spellingShingle | evaluation antigens design formulation Riitho, Victor Walters, Adam A. Somavarapu, Satyanarayana Lamp, Benjamin Rümenapf, Till Krey, Thomas Rey, Felix A. Oviedo-Orta, Ernesto Stewart, Graham R. Locker, Nicolas Steinbach, Falko Graham, Simon P. Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens |
| title | Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens |
| title_full | Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens |
| title_fullStr | Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens |
| title_full_unstemmed | Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens |
| title_short | Design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens |
| title_sort | design and evaluation of the immunogenicity and efficacy of a biomimetic particulate formulation of viral antigens |
| topic | evaluation antigens design formulation |
| url | https://hdl.handle.net/10568/129303 |
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