Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections
Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2004
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/129298 |
| _version_ | 1855513837689634816 |
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| author | Claes, Filip Radwanska, Magda Urakawa, Toyo Majiwa, Phelix A.O. Goddeeris, Bruno M. Büscher, Philippe |
| author_browse | Büscher, Philippe Claes, Filip Goddeeris, Bruno M. Majiwa, Phelix A.O. Radwanska, Magda Urakawa, Toyo |
| author_facet | Claes, Filip Radwanska, Magda Urakawa, Toyo Majiwa, Phelix A.O. Goddeeris, Bruno M. Büscher, Philippe |
| author_sort | Claes, Filip |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR).This PCR yielded a 205 bp product in all T. evansi and in seven out of nine T. equiperdum strains tested. This product was not detected in the DNA from T. b. brucei, T. b. gambiense, T. b. rhodesiense, T. congolense, T. vivax and T. theileri parasites. The Rotat 1.2 PCR detects as few as 10 trypanosomes per reaction with purified DNA from blood samples, i.e. 50 trypanosomes/ml.PCR amplification of the RoTat 1.2 VSG gene is a specific marker for T. evansi strains, except T. evansi type B, and is especially useful in dyskinetoplastic strains where kDNA based markers may fail to amplify. Furthermore, our data support previous suggestions that some T. evansi stocks have been previously misclassified as T. equiperdum. |
| format | Journal Article |
| id | CGSpace129298 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1292982025-12-08T09:54:28Z Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections Claes, Filip Radwanska, Magda Urakawa, Toyo Majiwa, Phelix A.O. Goddeeris, Bruno M. Büscher, Philippe pcr trypanosoma evansi detection glycoprotein diagnostic Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR).This PCR yielded a 205 bp product in all T. evansi and in seven out of nine T. equiperdum strains tested. This product was not detected in the DNA from T. b. brucei, T. b. gambiense, T. b. rhodesiense, T. congolense, T. vivax and T. theileri parasites. The Rotat 1.2 PCR detects as few as 10 trypanosomes per reaction with purified DNA from blood samples, i.e. 50 trypanosomes/ml.PCR amplification of the RoTat 1.2 VSG gene is a specific marker for T. evansi strains, except T. evansi type B, and is especially useful in dyskinetoplastic strains where kDNA based markers may fail to amplify. Furthermore, our data support previous suggestions that some T. evansi stocks have been previously misclassified as T. equiperdum. 2004 2023-03-10T14:33:02Z 2023-03-10T14:33:02Z Journal Article https://hdl.handle.net/10568/129298 en Open Access Springer Claes, Filip; Radwanska, Magda; Urakawa, Toyo; Majiwa, Phelix A.O.; Goddeeris, Bruno M.; Büscher, Philippe. 2004. Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections. Kinetoplastid Biology and Disease 3: 3 |
| spellingShingle | pcr trypanosoma evansi detection glycoprotein diagnostic Claes, Filip Radwanska, Magda Urakawa, Toyo Majiwa, Phelix A.O. Goddeeris, Bruno M. Büscher, Philippe Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections |
| title | Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections |
| title_full | Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections |
| title_fullStr | Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections |
| title_full_unstemmed | Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections |
| title_short | Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections |
| title_sort | variable surface glycoprotein rotat 1 2 pcr as a specific diagnostic tool for the detection of trypanosoma evansi infections |
| topic | pcr trypanosoma evansi detection glycoprotein diagnostic |
| url | https://hdl.handle.net/10568/129298 |
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