Validation of Novel spot blotch disease resistance alleles identified in unexplored wheat (Triticum aestivum L.) germplasm lines through KASP markers
Background: During the last few decades, the diverse sources of resistance, several genes and QTLs for spot blotch resistance have been identified. However, a large set of germplasm lines are still unexplored that have the potential to develop highly resistant wheat cultivars for the target environm...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/129204 |
| _version_ | 1855518785236107264 |
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| author | Suneel Kumar Pradhan, Anjan Kumar Kumar, Uttam Dhillon, Guriqbal Singh Kaur, Satinder Neeraj Budhlakoti Dwijesh Chandra Mishra Singh, Amit Kumar Singh, Rakesh Kumari, Jyoti Kumaran, Vikas V. Vinod Kumar Mishra Bhati, Pradeep Das, Saikat Chand, Ramesh Singh, Kuldeep Kumar, Sundeep |
| author_browse | Bhati, Pradeep Chand, Ramesh Das, Saikat Dhillon, Guriqbal Singh Dwijesh Chandra Mishra Kaur, Satinder Kumar, Sundeep Kumar, Uttam Kumaran, Vikas V. Kumari, Jyoti Neeraj Budhlakoti Pradhan, Anjan Kumar Singh, Amit Kumar Singh, Kuldeep Singh, Rakesh Suneel Kumar Vinod Kumar Mishra |
| author_facet | Suneel Kumar Pradhan, Anjan Kumar Kumar, Uttam Dhillon, Guriqbal Singh Kaur, Satinder Neeraj Budhlakoti Dwijesh Chandra Mishra Singh, Amit Kumar Singh, Rakesh Kumari, Jyoti Kumaran, Vikas V. Vinod Kumar Mishra Bhati, Pradeep Das, Saikat Chand, Ramesh Singh, Kuldeep Kumar, Sundeep |
| author_sort | Suneel Kumar |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background: During the last few decades, the diverse sources of resistance, several genes and QTLs for spot blotch resistance have been identified. However, a large set of germplasm lines are still unexplored that have the potential to develop highly resistant wheat cultivars for the target environments. Therefore, the identification of new sources of resistance to spot blotch is essential for breeding programmes to develop spot blotch resistant cultivars and sustain wheat production. The association mapping panel of 294 diverse bread wheat accessions was used to explore new sources of spot blotch disease resistance and to identify genomic regions using genome wide association analysis (GWAS). The genotypes were tested in replicated trials for spot blotch disease at three major hot spots in India (Varanasi in UP, Pusa in Bihar, and Cooch Behar in West Bengal). The area under the disease progress curve (AUDPC) was calculated to assess the level of resistance in each genotype. Results: A total of 19 highly and 76 moderately resistant lines were identified. Three accessions (EC664204, IC534306 and IC535188) were nearly immune to spot blotch disease. The genotyping of all accessions resulted in a total of 16,787 high-quality polymorphic SNPs. The GWAS was performed using a Compressed Mixed Linear Model (CMLM) and a Mixed Linear Model (MLM). A total of seven significant MTAs, common in both the models and consistent across the environment, were further validated to develop KASP markers. Four MTAs (AX-94710084, AX-94865722, AX-95135556, and AX-94529408) on three chromosomes (2AL, 2BL, and 3BL) have been successfully validated through the KASP marker. Conclusions: The new source of resistance was identified from unexplored germplasm lines. The genomic regions identified through GWAS were validated through KASP markers. The marker information and the highly resistant sources are valuable resources to rapidly develop immune or near immune wheat varieties. |
| format | Journal Article |
| id | CGSpace129204 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1292042025-12-08T09:54:28Z Validation of Novel spot blotch disease resistance alleles identified in unexplored wheat (Triticum aestivum L.) germplasm lines through KASP markers Suneel Kumar Pradhan, Anjan Kumar Kumar, Uttam Dhillon, Guriqbal Singh Kaur, Satinder Neeraj Budhlakoti Dwijesh Chandra Mishra Singh, Amit Kumar Singh, Rakesh Kumari, Jyoti Kumaran, Vikas V. Vinod Kumar Mishra Bhati, Pradeep Das, Saikat Chand, Ramesh Singh, Kuldeep Kumar, Sundeep triticum aestivum material transfer agreements marker-assisted selection disease resistance wheat Background: During the last few decades, the diverse sources of resistance, several genes and QTLs for spot blotch resistance have been identified. However, a large set of germplasm lines are still unexplored that have the potential to develop highly resistant wheat cultivars for the target environments. Therefore, the identification of new sources of resistance to spot blotch is essential for breeding programmes to develop spot blotch resistant cultivars and sustain wheat production. The association mapping panel of 294 diverse bread wheat accessions was used to explore new sources of spot blotch disease resistance and to identify genomic regions using genome wide association analysis (GWAS). The genotypes were tested in replicated trials for spot blotch disease at three major hot spots in India (Varanasi in UP, Pusa in Bihar, and Cooch Behar in West Bengal). The area under the disease progress curve (AUDPC) was calculated to assess the level of resistance in each genotype. Results: A total of 19 highly and 76 moderately resistant lines were identified. Three accessions (EC664204, IC534306 and IC535188) were nearly immune to spot blotch disease. The genotyping of all accessions resulted in a total of 16,787 high-quality polymorphic SNPs. The GWAS was performed using a Compressed Mixed Linear Model (CMLM) and a Mixed Linear Model (MLM). A total of seven significant MTAs, common in both the models and consistent across the environment, were further validated to develop KASP markers. Four MTAs (AX-94710084, AX-94865722, AX-95135556, and AX-94529408) on three chromosomes (2AL, 2BL, and 3BL) have been successfully validated through the KASP marker. Conclusions: The new source of resistance was identified from unexplored germplasm lines. The genomic regions identified through GWAS were validated through KASP markers. The marker information and the highly resistant sources are valuable resources to rapidly develop immune or near immune wheat varieties. 2022 2023-03-06T14:46:38Z 2023-03-06T14:46:38Z Journal Article https://hdl.handle.net/10568/129204 en Open Access application/pdf Springer Kumar, S., Pradhan, A. K., Kumar, U., Dhillon, G. S., Kaur, S., Budhlakoti, N., Mishra, D. C., Singh, A. K., Singh, R., Kumari, J., Kumaran, V. V., Mishra, V. K., Bhati, P. K., Das, S., Chand, R., Singh, K., & Kumar, S. (2022). Validation of Novel spot blotch disease resistance alleles identified in unexplored wheat (Triticum aestivum L.) germplasm lines through KASP markers. BMC Plant Biology, 22(1). https://doi.org/10.1186/s12870-022-04013-w |
| spellingShingle | triticum aestivum material transfer agreements marker-assisted selection disease resistance wheat Suneel Kumar Pradhan, Anjan Kumar Kumar, Uttam Dhillon, Guriqbal Singh Kaur, Satinder Neeraj Budhlakoti Dwijesh Chandra Mishra Singh, Amit Kumar Singh, Rakesh Kumari, Jyoti Kumaran, Vikas V. Vinod Kumar Mishra Bhati, Pradeep Das, Saikat Chand, Ramesh Singh, Kuldeep Kumar, Sundeep Validation of Novel spot blotch disease resistance alleles identified in unexplored wheat (Triticum aestivum L.) germplasm lines through KASP markers |
| title | Validation of Novel spot blotch disease resistance alleles identified in unexplored wheat (Triticum aestivum L.) germplasm lines through KASP markers |
| title_full | Validation of Novel spot blotch disease resistance alleles identified in unexplored wheat (Triticum aestivum L.) germplasm lines through KASP markers |
| title_fullStr | Validation of Novel spot blotch disease resistance alleles identified in unexplored wheat (Triticum aestivum L.) germplasm lines through KASP markers |
| title_full_unstemmed | Validation of Novel spot blotch disease resistance alleles identified in unexplored wheat (Triticum aestivum L.) germplasm lines through KASP markers |
| title_short | Validation of Novel spot blotch disease resistance alleles identified in unexplored wheat (Triticum aestivum L.) germplasm lines through KASP markers |
| title_sort | validation of novel spot blotch disease resistance alleles identified in unexplored wheat triticum aestivum l germplasm lines through kasp markers |
| topic | triticum aestivum material transfer agreements marker-assisted selection disease resistance wheat |
| url | https://hdl.handle.net/10568/129204 |
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