Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii
The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMM...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2022
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/129179 |
| _version_ | 1855533112384028672 |
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| author | Lin, Guifang Hui Chen Bin Tian Sehgal, Sunish Kumar Singh, Lovepreet K. Jingzhong Xie Rawat, Nidhi Juliana, Philomin Narinder Singh Shrestha, Sandesh Wilson, Duane L. Shult, Hannah Lee, Hyeonju Schoen, Adam William Tiwari, Vijay Kumar Singh, Ravi P. Guttieri, Mary J. Trick, Harold N. Poland, Jesse A. Bowden, Robert L. Guihua Bai Gill, Bikram S. Liu, Sanzhen |
| author_browse | Bin Tian Bowden, Robert L. Gill, Bikram S. Guihua Bai Guttieri, Mary J. Hui Chen Jingzhong Xie Juliana, Philomin Lee, Hyeonju Lin, Guifang Liu, Sanzhen Narinder Singh Poland, Jesse A. Rawat, Nidhi Schoen, Adam William Sehgal, Sunish Kumar Shrestha, Sandesh Shult, Hannah Singh, Lovepreet K. Singh, Ravi P. Tiwari, Vijay Kumar Trick, Harold N. Wilson, Duane L. |
| author_facet | Lin, Guifang Hui Chen Bin Tian Sehgal, Sunish Kumar Singh, Lovepreet K. Jingzhong Xie Rawat, Nidhi Juliana, Philomin Narinder Singh Shrestha, Sandesh Wilson, Duane L. Shult, Hannah Lee, Hyeonju Schoen, Adam William Tiwari, Vijay Kumar Singh, Ravi P. Guttieri, Mary J. Trick, Harold N. Poland, Jesse A. Bowden, Robert L. Guihua Bai Gill, Bikram S. Liu, Sanzhen |
| author_sort | Lin, Guifang |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement. |
| format | Journal Article |
| id | CGSpace129179 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1291792025-11-06T13:07:40Z Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii Lin, Guifang Hui Chen Bin Tian Sehgal, Sunish Kumar Singh, Lovepreet K. Jingzhong Xie Rawat, Nidhi Juliana, Philomin Narinder Singh Shrestha, Sandesh Wilson, Duane L. Shult, Hannah Lee, Hyeonju Schoen, Adam William Tiwari, Vijay Kumar Singh, Ravi P. Guttieri, Mary J. Trick, Harold N. Poland, Jesse A. Bowden, Robert L. Guihua Bai Gill, Bikram S. Liu, Sanzhen alleles clones gene expression grasses mutation rusts wheat basidiomycota disease resistance genetics molecular cloning plant breeding plant diseases The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement. 2022-06-01 2023-03-03T16:17:49Z 2023-03-03T16:17:49Z Journal Article https://hdl.handle.net/10568/129179 en Open Access application/pdf Springer Lin, G., Chen, H., Tian, B., Sehgal, S. K., Singh, L., Xie, J., Rawat, N., Juliana, P., Singh, N., Shrestha, S., Wilson, D. L., Shult, H., Lee, H., Schoen, A. W., Tiwari, V. K., Singh, R. P., Guttieri, M. J., Trick, H. N., Poland, J., … Liu, S. (2022). Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-30784-9 |
| spellingShingle | alleles clones gene expression grasses mutation rusts wheat basidiomycota disease resistance genetics molecular cloning plant breeding plant diseases Lin, Guifang Hui Chen Bin Tian Sehgal, Sunish Kumar Singh, Lovepreet K. Jingzhong Xie Rawat, Nidhi Juliana, Philomin Narinder Singh Shrestha, Sandesh Wilson, Duane L. Shult, Hannah Lee, Hyeonju Schoen, Adam William Tiwari, Vijay Kumar Singh, Ravi P. Guttieri, Mary J. Trick, Harold N. Poland, Jesse A. Bowden, Robert L. Guihua Bai Gill, Bikram S. Liu, Sanzhen Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii |
| title | Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii |
| title_full | Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii |
| title_fullStr | Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii |
| title_full_unstemmed | Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii |
| title_short | Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii |
| title_sort | cloning of the broadly effective wheat leaf rust resistance gene lr42 transferred from aegilops tauschii |
| topic | alleles clones gene expression grasses mutation rusts wheat basidiomycota disease resistance genetics molecular cloning plant breeding plant diseases |
| url | https://hdl.handle.net/10568/129179 |
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