Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci
Developing wheat varieties with durable resistance is a core objective of the International Maize and Wheat Improvement Center (CIMMYT) and many other breeding programs worldwide. The CIMMYT advanced wheat line “Mucuy” displayed high levels of resistance to stripe rust (YR) and leaf rust (LR) in fie...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Frontiers Media
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/129175 |
| _version_ | 1855532455070531584 |
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| author | Demei Liu Chan Yuan Singh, Ravi P. Randhawa, Mandeep S. Bhavani, Sridhar Kumar, Uttam Huerta Espino, Julio Lagudah, Evans S. Caixia Lan |
| author_browse | Bhavani, Sridhar Caixia Lan Chan Yuan Demei Liu Huerta Espino, Julio Kumar, Uttam Lagudah, Evans S. Randhawa, Mandeep S. Singh, Ravi P. |
| author_facet | Demei Liu Chan Yuan Singh, Ravi P. Randhawa, Mandeep S. Bhavani, Sridhar Kumar, Uttam Huerta Espino, Julio Lagudah, Evans S. Caixia Lan |
| author_sort | Demei Liu |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Developing wheat varieties with durable resistance is a core objective of the International Maize and Wheat Improvement Center (CIMMYT) and many other breeding programs worldwide. The CIMMYT advanced wheat line “Mucuy” displayed high levels of resistance to stripe rust (YR) and leaf rust (LR) in field evaluations in Mexico and several other countries. To determine the genetic basis of YR and LR resistance, 138 F5 recombinant inbred lines (RILs) derived from the cross of Apav#1× Mucuy were phenotyped for YR responses from 2015 to 2020 at field sites in India, Kenya, and Mexico, and LR in Mexico. Seedling phenotyping for YR and LR responses was conducted in the greenhouse in Mexico using the same predominant races as in field trials. Using 12,681 polymorphic molecular markers from the DArT, SNP, and SSR genotyping platforms, we constructed genetic linkage maps and QTL analyses that detected seven YR and four LR resistance loci. Among these, a co-located YR/LR resistance loci was identified as Yr29/Lr46, and a seedling stripe rust resistance gene YrMu was mapped on the 2AS/2NS translocation. This fragment also conferred moderate adult plant resistance (APR) under all Mexican field environments and in one season in Kenya. Field trial phenotyping with Lr37-virulent Puccinia triticina races indicated the presence of an APR QTL accounting for 18.3–25.5% of the LR severity variation, in addition to a novel YR resistance QTL, QYr.cim-3DS, derived from Mucuy. We developed breeder-friendly KASP and indel molecular markers respectively for Yr29/Lr46 and YrMu. The current study validated the presence of known genes and identified new resistance loci, a QTL combination effect, and flanking markers to facilitate accelerated breeding for genetically complex, durable rust resistance. |
| format | Journal Article |
| id | CGSpace129175 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1291752025-12-08T10:29:22Z Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci Demei Liu Chan Yuan Singh, Ravi P. Randhawa, Mandeep S. Bhavani, Sridhar Kumar, Uttam Huerta Espino, Julio Lagudah, Evans S. Caixia Lan rusts puccinia striiformis quantitative trait loci adult plant resistance wheat Developing wheat varieties with durable resistance is a core objective of the International Maize and Wheat Improvement Center (CIMMYT) and many other breeding programs worldwide. The CIMMYT advanced wheat line “Mucuy” displayed high levels of resistance to stripe rust (YR) and leaf rust (LR) in field evaluations in Mexico and several other countries. To determine the genetic basis of YR and LR resistance, 138 F5 recombinant inbred lines (RILs) derived from the cross of Apav#1× Mucuy were phenotyped for YR responses from 2015 to 2020 at field sites in India, Kenya, and Mexico, and LR in Mexico. Seedling phenotyping for YR and LR responses was conducted in the greenhouse in Mexico using the same predominant races as in field trials. Using 12,681 polymorphic molecular markers from the DArT, SNP, and SSR genotyping platforms, we constructed genetic linkage maps and QTL analyses that detected seven YR and four LR resistance loci. Among these, a co-located YR/LR resistance loci was identified as Yr29/Lr46, and a seedling stripe rust resistance gene YrMu was mapped on the 2AS/2NS translocation. This fragment also conferred moderate adult plant resistance (APR) under all Mexican field environments and in one season in Kenya. Field trial phenotyping with Lr37-virulent Puccinia triticina races indicated the presence of an APR QTL accounting for 18.3–25.5% of the LR severity variation, in addition to a novel YR resistance QTL, QYr.cim-3DS, derived from Mucuy. We developed breeder-friendly KASP and indel molecular markers respectively for Yr29/Lr46 and YrMu. The current study validated the presence of known genes and identified new resistance loci, a QTL combination effect, and flanking markers to facilitate accelerated breeding for genetically complex, durable rust resistance. 2022-08-19 2023-03-03T16:17:26Z 2023-03-03T16:17:26Z Journal Article https://hdl.handle.net/10568/129175 en Open Access application/pdf Frontiers Media Liu, D., Yuan, C., Singh, R. P., Randhawa, M. S., Bhavani, S., Kumar, U., Huerta-Espino, J., Lagudah, E., & Lan, C. (2022). Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.880138 |
| spellingShingle | rusts puccinia striiformis quantitative trait loci adult plant resistance wheat Demei Liu Chan Yuan Singh, Ravi P. Randhawa, Mandeep S. Bhavani, Sridhar Kumar, Uttam Huerta Espino, Julio Lagudah, Evans S. Caixia Lan Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci |
| title | Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci |
| title_full | Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci |
| title_fullStr | Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci |
| title_full_unstemmed | Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci |
| title_short | Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci |
| title_sort | stripe rust and leaf rust resistance in cimmyt wheat line mucuy is conferred by combinations of race specific and adult plant resistance loci |
| topic | rusts puccinia striiformis quantitative trait loci adult plant resistance wheat |
| url | https://hdl.handle.net/10568/129175 |
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