Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat
Wheat (Tritium aestivum L.) productivity is severely hampered by various pathogens and changing climatic conditions. Spot blotch and terminal heat stress are the major constraints of wheat production in the eastern Gangetic plains of India. To identify novel breeding sources and to understand underl...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/129096 |
| _version_ | 1855528762189283328 |
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| author | Singh, G. Mahendra SrinathaReddy S Sharma, Gaurav Bakshi, Suman Kumar, Uttam Bhati, Pradeep Jambhulkar, Sanjay J. Chand, Ramesh Joshi, Arun Kumar Vinod Kumar Mishra Sandeep Sharma |
| author_browse | Bakshi, Suman Bhati, Pradeep Chand, Ramesh Jambhulkar, Sanjay J. Joshi, Arun Kumar Kumar, Uttam Sandeep Sharma Sharma, Gaurav Singh, G. Mahendra SrinathaReddy S Vinod Kumar Mishra |
| author_facet | Singh, G. Mahendra SrinathaReddy S Sharma, Gaurav Bakshi, Suman Kumar, Uttam Bhati, Pradeep Jambhulkar, Sanjay J. Chand, Ramesh Joshi, Arun Kumar Vinod Kumar Mishra Sandeep Sharma |
| author_sort | Singh, G. Mahendra |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Wheat (Tritium aestivum L.) productivity is severely hampered by various pathogens and changing climatic conditions. Spot blotch and terminal heat stress are the major constraints of wheat production in the eastern Gangetic plains of India. To identify novel breeding sources and to understand underlying resistance mechanisms, forty-four gamma rays mutagenized wheat genotypes, derived from three different parents were screened under favourable agro-ecological conditions for spot blotch and terminal heat stress. Ten mutants showed reduced spot blotch infection calculated based on Area Under Disease Progress Curve (AUDPC), than their respective parents. The mutant TAW41 had the least infection (AUDPC: 354.32), significantly lower than its parent HD2967 (AUDPC: 675.51) and other checks. TAW41 also had a higher Normalized Difference Vegetation Index (NDVI) and chlorophyll content than the parent. Gene expression analysis of TAW41 showed differential accumulation of transcripts involved in hormonal pathways (Salicylic acid, Jasmonic acid, and ethylene) and other defense-associated genes, indicating that TAW41 might have unique resistance mechanism that facilitates this genotype to perform better against the combined stress of spot blotch and terminal heat. Hence, mutant TAW41 has been identified as a novel source of resistance that could be exploited in wheat improvement programmes to enhance tolerance to spot blotch and terminal heat stress. |
| format | Journal Article |
| id | CGSpace129096 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1290962025-11-06T13:06:26Z Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat Singh, G. Mahendra SrinathaReddy S Sharma, Gaurav Bakshi, Suman Kumar, Uttam Bhati, Pradeep Jambhulkar, Sanjay J. Chand, Ramesh Joshi, Arun Kumar Vinod Kumar Mishra Sandeep Sharma wheat mutants spots heat gene expression hormones Wheat (Tritium aestivum L.) productivity is severely hampered by various pathogens and changing climatic conditions. Spot blotch and terminal heat stress are the major constraints of wheat production in the eastern Gangetic plains of India. To identify novel breeding sources and to understand underlying resistance mechanisms, forty-four gamma rays mutagenized wheat genotypes, derived from three different parents were screened under favourable agro-ecological conditions for spot blotch and terminal heat stress. Ten mutants showed reduced spot blotch infection calculated based on Area Under Disease Progress Curve (AUDPC), than their respective parents. The mutant TAW41 had the least infection (AUDPC: 354.32), significantly lower than its parent HD2967 (AUDPC: 675.51) and other checks. TAW41 also had a higher Normalized Difference Vegetation Index (NDVI) and chlorophyll content than the parent. Gene expression analysis of TAW41 showed differential accumulation of transcripts involved in hormonal pathways (Salicylic acid, Jasmonic acid, and ethylene) and other defense-associated genes, indicating that TAW41 might have unique resistance mechanism that facilitates this genotype to perform better against the combined stress of spot blotch and terminal heat. Hence, mutant TAW41 has been identified as a novel source of resistance that could be exploited in wheat improvement programmes to enhance tolerance to spot blotch and terminal heat stress. 2022-01 2023-02-28T09:00:10Z 2023-02-28T09:00:10Z Journal Article https://hdl.handle.net/10568/129096 en Open Access application/pdf Elsevier Singh, G. M., S, S., Sharma, G., Bakshi, S., Kumar, U., Bhati, P., Jambhulkar, S. J., Chand, R., Joshi, A. K., Mishra, V. K., & Sharma, S. (2022). Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat. Current Plant Biology, 29, 100234. https://doi.org/10.1016/j.cpb.2021.100234 |
| spellingShingle | wheat mutants spots heat gene expression hormones Singh, G. Mahendra SrinathaReddy S Sharma, Gaurav Bakshi, Suman Kumar, Uttam Bhati, Pradeep Jambhulkar, Sanjay J. Chand, Ramesh Joshi, Arun Kumar Vinod Kumar Mishra Sandeep Sharma Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_full | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_fullStr | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_full_unstemmed | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_short | Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| title_sort | expression analysis of hormonal pathways and defense associated genes in gamma rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat |
| topic | wheat mutants spots heat gene expression hormones |
| url | https://hdl.handle.net/10568/129096 |
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