Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL
Thousand kernel weight (TKW) is an important yield component trait in wheat, and identification of the underlying genetic loci is helpful for yield improvement. We previously identified a stable quantitative trait locus (QTL) QTKW.caas-5DL for TKW in a Doumai/Shi4185 recombinant inbred line (RIL) po...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2022
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/129062 |
| _version_ | 1855541833239625728 |
|---|---|
| author | Jie Song Dengan Xu Yan Dong Faji Li Bian, Yingjie Lingling Li Xumei Luo Fei, Shuaipeng Lei Li Cong Zhao Yong Zhang Xianchun Xia Zhongfu Ni He Zhonghu Shuanghe Cao |
| author_browse | Bian, Yingjie Cong Zhao Dengan Xu Faji Li Fei, Shuaipeng He Zhonghu Jie Song Lei Li Lingling Li Shuanghe Cao Xianchun Xia Xumei Luo Yan Dong Yong Zhang Zhongfu Ni |
| author_facet | Jie Song Dengan Xu Yan Dong Faji Li Bian, Yingjie Lingling Li Xumei Luo Fei, Shuaipeng Lei Li Cong Zhao Yong Zhang Xianchun Xia Zhongfu Ni He Zhonghu Shuanghe Cao |
| author_sort | Jie Song |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Thousand kernel weight (TKW) is an important yield component trait in wheat, and identification of the underlying genetic loci is helpful for yield improvement. We previously identified a stable quantitative trait locus (QTL) QTKW.caas-5DL for TKW in a Doumai/Shi4185 recombinant inbred line (RIL) population. Here we performed fine mapping of QTKW.caas-5DL using secondary populations derived from 15 heterozygous recombinants and delimited the QTL to an approximate 3.9 Mb physical interval from 409.9 to 413.8 Mb according to the Chinese Spring (CS) reference genome. Analysis of genomic synteny showed that annotated genes in the physical interval had high collinearity among CS and eight other wheat genomes. Seven genes with sequence variation and/or differential expression between parents were predicted as candidates for QTKW.caas-5DL based on whole-genome resequencing and transcriptome assays. A kompetitive allele-specific PCR (KASP) marker for QTKW.caas-5DL was developed, and genotyping confirmed a significant association with TKW but not with other yield component traits in a panel of elite wheat cultivars. The superior allele of QTKW.caas-5DL was frequent in a panel of cultivars, suggesting that it had undergone positive selection. These findings not only lay a foundation for map-based cloning of QTKW.caas-5DL but also provide an efficient tool for marker-assisted selection. |
| format | Journal Article |
| id | CGSpace129062 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1290622025-03-11T12:14:31Z Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL Jie Song Dengan Xu Yan Dong Faji Li Bian, Yingjie Lingling Li Xumei Luo Fei, Shuaipeng Lei Li Cong Zhao Yong Zhang Xianchun Xia Zhongfu Ni He Zhonghu Shuanghe Cao quantitative trait loci wheat yields improvement genomes marker-assisted selection Thousand kernel weight (TKW) is an important yield component trait in wheat, and identification of the underlying genetic loci is helpful for yield improvement. We previously identified a stable quantitative trait locus (QTL) QTKW.caas-5DL for TKW in a Doumai/Shi4185 recombinant inbred line (RIL) population. Here we performed fine mapping of QTKW.caas-5DL using secondary populations derived from 15 heterozygous recombinants and delimited the QTL to an approximate 3.9 Mb physical interval from 409.9 to 413.8 Mb according to the Chinese Spring (CS) reference genome. Analysis of genomic synteny showed that annotated genes in the physical interval had high collinearity among CS and eight other wheat genomes. Seven genes with sequence variation and/or differential expression between parents were predicted as candidates for QTKW.caas-5DL based on whole-genome resequencing and transcriptome assays. A kompetitive allele-specific PCR (KASP) marker for QTKW.caas-5DL was developed, and genotyping confirmed a significant association with TKW but not with other yield component traits in a panel of elite wheat cultivars. The superior allele of QTKW.caas-5DL was frequent in a panel of cultivars, suggesting that it had undergone positive selection. These findings not only lay a foundation for map-based cloning of QTKW.caas-5DL but also provide an efficient tool for marker-assisted selection. 2022-09 2023-02-26T17:36:13Z 2023-02-26T17:36:13Z Journal Article https://hdl.handle.net/10568/129062 en Limited Access Springer Song, J., Xu, D., Dong, Y., Li, F., Bian, Y., Li, L., Luo, X., Fei, S., Li, L., Zhao, C., Zhang, Y., Xia, X., Ni, Z., He, Z. and Cao, S. 2022. Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL. Theoretical and Applied Genetics 135(9):3237–3246. |
| spellingShingle | quantitative trait loci wheat yields improvement genomes marker-assisted selection Jie Song Dengan Xu Yan Dong Faji Li Bian, Yingjie Lingling Li Xumei Luo Fei, Shuaipeng Lei Li Cong Zhao Yong Zhang Xianchun Xia Zhongfu Ni He Zhonghu Shuanghe Cao Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL |
| title | Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL |
| title_full | Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL |
| title_fullStr | Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL |
| title_full_unstemmed | Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL |
| title_short | Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL |
| title_sort | fine mapping and characterization of a major qtl for grain weight on wheat chromosome arm 5dl |
| topic | quantitative trait loci wheat yields improvement genomes marker-assisted selection |
| url | https://hdl.handle.net/10568/129062 |
| work_keys_str_mv | AT jiesong finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT denganxu finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT yandong finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT fajili finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT bianyingjie finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT linglingli finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT xumeiluo finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT feishuaipeng finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT leili finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT congzhao finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT yongzhang finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT xianchunxia finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT zhongfuni finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT hezhonghu finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl AT shuanghecao finemappingandcharacterizationofamajorqtlforgrainweightonwheatchromosomearm5dl |