Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat
Rht-B1b and Rht-D1b, the ‘Green Revolution’ (GR) genes, greatly improved yield potential of wheat under nitrogen fertilizer application, but reduced coleoptile length, seedling vigor and grain weight. Thus, mining alternative reduced plant height genes without adverse effects is urgently needed. We...
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2022
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/129061 |
| _version_ | 1855527357922672640 |
|---|---|
| author | Xiuling Tian Xianchun Xia Dengan Xu Yongqiang Liu Li Xie Hassan, Muhammad Adeel Jie Song Faji Li Desen Wang Yong Zhang Yuanfeng Hao Genying Li Chengcai Chu He Zhonghu Shuanghe Cao |
| author_browse | Chengcai Chu Dengan Xu Desen Wang Faji Li Genying Li Hassan, Muhammad Adeel He Zhonghu Jie Song Li Xie Shuanghe Cao Xianchun Xia Xiuling Tian Yong Zhang Yongqiang Liu Yuanfeng Hao |
| author_facet | Xiuling Tian Xianchun Xia Dengan Xu Yongqiang Liu Li Xie Hassan, Muhammad Adeel Jie Song Faji Li Desen Wang Yong Zhang Yuanfeng Hao Genying Li Chengcai Chu He Zhonghu Shuanghe Cao |
| author_sort | Xiuling Tian |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rht-B1b and Rht-D1b, the ‘Green Revolution’ (GR) genes, greatly improved yield potential of wheat under nitrogen fertilizer application, but reduced coleoptile length, seedling vigor and grain weight. Thus, mining alternative reduced plant height genes without adverse effects is urgently needed. We isolated the causal gene of Rht24 through map-based cloning and characterized its function using transgenic, physiobiochemical and transcriptome assays. We confirmed genetic effects of the dwarfing allele Rht24b with an association analysis and also traced its origin and distribution. Rht24 encodes a gibberellin (GA) 2-oxidase, TaGA2ox-A9. Rht24b conferred higher expression of TaGA2ox-A9 in stems, leading to a reduction of bioactive GA in stems but an elevation in leaves at the jointing stage. Strikingly, Rht24b reduced plant height, but had no yield penalty; it significantly increased nitrogen use efficiency, photosynthetic rate and the expression of related genes. Evolutionary analysis demonstrated that Rht24b first appeared in wild emmer and was detected in more than half of wild emmer and wheat accessions, suggesting that it underwent both natural and artificial selection. These findings uncover an important genetic resource for wheat breeding and also provide clues for dissecting the regulatory mechanisms underlying GA-mediated morphogenesis and yield formation. |
| format | Journal Article |
| id | CGSpace129061 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1290612025-03-11T12:14:31Z Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat Xiuling Tian Xianchun Xia Dengan Xu Yongqiang Liu Li Xie Hassan, Muhammad Adeel Jie Song Faji Li Desen Wang Yong Zhang Yuanfeng Hao Genying Li Chengcai Chu He Zhonghu Shuanghe Cao gibberellins oxidoreductases grain plant height genes wheat Rht-B1b and Rht-D1b, the ‘Green Revolution’ (GR) genes, greatly improved yield potential of wheat under nitrogen fertilizer application, but reduced coleoptile length, seedling vigor and grain weight. Thus, mining alternative reduced plant height genes without adverse effects is urgently needed. We isolated the causal gene of Rht24 through map-based cloning and characterized its function using transgenic, physiobiochemical and transcriptome assays. We confirmed genetic effects of the dwarfing allele Rht24b with an association analysis and also traced its origin and distribution. Rht24 encodes a gibberellin (GA) 2-oxidase, TaGA2ox-A9. Rht24b conferred higher expression of TaGA2ox-A9 in stems, leading to a reduction of bioactive GA in stems but an elevation in leaves at the jointing stage. Strikingly, Rht24b reduced plant height, but had no yield penalty; it significantly increased nitrogen use efficiency, photosynthetic rate and the expression of related genes. Evolutionary analysis demonstrated that Rht24b first appeared in wild emmer and was detected in more than half of wild emmer and wheat accessions, suggesting that it underwent both natural and artificial selection. These findings uncover an important genetic resource for wheat breeding and also provide clues for dissecting the regulatory mechanisms underlying GA-mediated morphogenesis and yield formation. 2022-01 2023-02-26T17:35:27Z 2023-02-26T17:35:27Z Journal Article https://hdl.handle.net/10568/129061 en Limited Access Wiley Tian, X., Xia, X., Xu, D., Liu, Y., Xie, L., Hassan, M. A., Song, J., Li, F., Wang, D., Zhang, Y., Hao, Y., Li, G., Chu, C., He, Z. and Cao, S. 2022. Rht24b, an ancient variation of taga2ox‐a9, reduces plant height without yield penalty in wheat. New Phytologist 233(2):738–750. |
| spellingShingle | gibberellins oxidoreductases grain plant height genes wheat Xiuling Tian Xianchun Xia Dengan Xu Yongqiang Liu Li Xie Hassan, Muhammad Adeel Jie Song Faji Li Desen Wang Yong Zhang Yuanfeng Hao Genying Li Chengcai Chu He Zhonghu Shuanghe Cao Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat |
| title | Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat |
| title_full | Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat |
| title_fullStr | Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat |
| title_full_unstemmed | Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat |
| title_short | Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat |
| title_sort | rht24b an ancient variation of taga2ox a9 reduces plant height without yield penalty in wheat |
| topic | gibberellins oxidoreductases grain plant height genes wheat |
| url | https://hdl.handle.net/10568/129061 |
| work_keys_str_mv | AT xiulingtian rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT xianchunxia rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT denganxu rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT yongqiangliu rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT lixie rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT hassanmuhammadadeel rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT jiesong rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT fajili rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT desenwang rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT yongzhang rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT yuanfenghao rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT genyingli rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT chengcaichu rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT hezhonghu rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat AT shuanghecao rht24banancientvariationoftaga2oxa9reducesplantheightwithoutyieldpenaltyinwheat |