Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL

Key message: YrFDC12 and PbcFDC, co-segregated in chromosome 4BL, and significantly interacted with Yr30/Pbc1 to enhance stripe rust resistance and to promote pseudo-black chaff development. Abstract: Cultivars with durable resistance are the most popular means to control wheat stripe rust. Durable...

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Autores principales: Shengjie Liu, Xiaoting Wang, Yayun Zhang, Yangang Jin, Zhonghua Xia, Mingjie Xiang, Shuo Huang, Linyi Qiao, Weijun Zheng, Qingdong Zeng, Qilin Wang, Rui Yu, Singh, Ravi P., Bhavani, Sridhar, Zhensheng Kang, Dejun Han, Changfa Wang, Jianhui Wu
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2022
Materias:
Acceso en línea:https://hdl.handle.net/10568/129056
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author Shengjie Liu
Xiaoting Wang
Yayun Zhang
Yangang Jin
Zhonghua Xia
Mingjie Xiang
Shuo Huang
Linyi Qiao
Weijun Zheng
Qingdong Zeng
Qilin Wang
Rui Yu
Singh, Ravi P.
Bhavani, Sridhar
Zhensheng Kang
Dejun Han
Changfa Wang
Jianhui Wu
author_browse Bhavani, Sridhar
Changfa Wang
Dejun Han
Jianhui Wu
Linyi Qiao
Mingjie Xiang
Qilin Wang
Qingdong Zeng
Rui Yu
Shengjie Liu
Shuo Huang
Singh, Ravi P.
Weijun Zheng
Xiaoting Wang
Yangang Jin
Yayun Zhang
Zhensheng Kang
Zhonghua Xia
author_facet Shengjie Liu
Xiaoting Wang
Yayun Zhang
Yangang Jin
Zhonghua Xia
Mingjie Xiang
Shuo Huang
Linyi Qiao
Weijun Zheng
Qingdong Zeng
Qilin Wang
Rui Yu
Singh, Ravi P.
Bhavani, Sridhar
Zhensheng Kang
Dejun Han
Changfa Wang
Jianhui Wu
author_sort Shengjie Liu
collection Repository of Agricultural Research Outputs (CGSpace)
description Key message: YrFDC12 and PbcFDC, co-segregated in chromosome 4BL, and significantly interacted with Yr30/Pbc1 to enhance stripe rust resistance and to promote pseudo-black chaff development. Abstract: Cultivars with durable resistance are the most popular means to control wheat stripe rust. Durable resistance can be achieved by stacking multiple adult plant resistance (APR) genes that individually have relatively small effect. Chinese wheat cultivars Ruihua 520 (RH520) and Fengdecun 12 (FDC12) confer partial APR to stripe rust across environments. One hundred and seventy recombinant inbred lines from the cross RH520 × FDC12 were used to determine the genetic basis of resistance and identify genomic regions associated with stripe rust resistance. Genotyping was carried out using 55 K SNP array, and eight quantitative trait loci (QTL) were detected on chromosome arms 2AL, 2DS, 3BS, 4BL, 5BL (2), and 7BL (2) by inclusive composite interval mapping. Only QYr.nwafu-3BS from RH520 and QYr.nwafu-4BL.2 (named YrFDC12 for convenience) from FDC12 were consistent across the four testing environments. QYr.nwafu-3BS is likely the pleiotropic resistance gene Sr2/Yr30. YrFDC12 was mapped in a 2.1-cM interval corresponding to 12 Mb and flanked by SNP markers AX-111121224 and AX-89518393. Lines harboring both Yr30 and YrFDC12 displayed higher resistance than the parents and expressed pseudo-black chaff (PBC) controlled by loci Pbc1 and PbcFDC12, which co-segregated with Yr30 and YrFDC12, respectively. Both marker-based and pedigree-based kinship analyses revealed that YrFDC12 was inherited from founder parent Zhou 8425B. Fifty-four other wheat cultivars shared the YrFDC12 haplotype. These results suggest an effective pyramiding strategy to acquire highly effective, durable stripe rust resistance in breeding.
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spelling CGSpace1290562024-08-27T10:35:40Z Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL Shengjie Liu Xiaoting Wang Yayun Zhang Yangang Jin Zhonghua Xia Mingjie Xiang Shuo Huang Linyi Qiao Weijun Zheng Qingdong Zeng Qilin Wang Rui Yu Singh, Ravi P. Bhavani, Sridhar Zhensheng Kang Dejun Han Changfa Wang Jianhui Wu genes genetic resistance quantitative trait loci inbred lines disease resistance rusts Key message: YrFDC12 and PbcFDC, co-segregated in chromosome 4BL, and significantly interacted with Yr30/Pbc1 to enhance stripe rust resistance and to promote pseudo-black chaff development. Abstract: Cultivars with durable resistance are the most popular means to control wheat stripe rust. Durable resistance can be achieved by stacking multiple adult plant resistance (APR) genes that individually have relatively small effect. Chinese wheat cultivars Ruihua 520 (RH520) and Fengdecun 12 (FDC12) confer partial APR to stripe rust across environments. One hundred and seventy recombinant inbred lines from the cross RH520 × FDC12 were used to determine the genetic basis of resistance and identify genomic regions associated with stripe rust resistance. Genotyping was carried out using 55 K SNP array, and eight quantitative trait loci (QTL) were detected on chromosome arms 2AL, 2DS, 3BS, 4BL, 5BL (2), and 7BL (2) by inclusive composite interval mapping. Only QYr.nwafu-3BS from RH520 and QYr.nwafu-4BL.2 (named YrFDC12 for convenience) from FDC12 were consistent across the four testing environments. QYr.nwafu-3BS is likely the pleiotropic resistance gene Sr2/Yr30. YrFDC12 was mapped in a 2.1-cM interval corresponding to 12 Mb and flanked by SNP markers AX-111121224 and AX-89518393. Lines harboring both Yr30 and YrFDC12 displayed higher resistance than the parents and expressed pseudo-black chaff (PBC) controlled by loci Pbc1 and PbcFDC12, which co-segregated with Yr30 and YrFDC12, respectively. Both marker-based and pedigree-based kinship analyses revealed that YrFDC12 was inherited from founder parent Zhou 8425B. Fifty-four other wheat cultivars shared the YrFDC12 haplotype. These results suggest an effective pyramiding strategy to acquire highly effective, durable stripe rust resistance in breeding. 2022-01 2023-02-26T17:35:11Z 2023-02-26T17:35:11Z Journal Article https://hdl.handle.net/10568/129056 en Limited Access Springer Liu, S., Wang, X., Zhang, Y., Jin, Y., Xia, Z., Xiang, M., Huang, S., Qiao, L., Zheng, W., Zeng, Q., Wang, Q., Yu, R., Singh, R.P., Bhavani, S., Kang, Z., Han, D., Wang, C. and Wu, J. 2022. Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL. Theoretical and Applied Genetics 135(1):351–365
spellingShingle genes
genetic resistance
quantitative trait loci
inbred lines
disease resistance
rusts
Shengjie Liu
Xiaoting Wang
Yayun Zhang
Yangang Jin
Zhonghua Xia
Mingjie Xiang
Shuo Huang
Linyi Qiao
Weijun Zheng
Qingdong Zeng
Qilin Wang
Rui Yu
Singh, Ravi P.
Bhavani, Sridhar
Zhensheng Kang
Dejun Han
Changfa Wang
Jianhui Wu
Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL
title Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL
title_full Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL
title_fullStr Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL
title_full_unstemmed Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL
title_short Enhanced stripe rust resistance obtained by combining Yr30 with a widely dispersed, consistent QTL on chromosome arm 4BL
title_sort enhanced stripe rust resistance obtained by combining yr30 with a widely dispersed consistent qtl on chromosome arm 4bl
topic genes
genetic resistance
quantitative trait loci
inbred lines
disease resistance
rusts
url https://hdl.handle.net/10568/129056
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