Monocotyledonous plants graft at the embryonic root–shoot interface
Grafting is possible in both animals and plants. Although in animals the process requires surgery and is often associated with rejection of non-self, in plants grafting is widespread, and has been used since antiquity for crop improvement1. However, in the monocotyledons, which represent the second...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2022
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/129040 |
| _version_ | 1855528353219477504 |
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| author | Reeves, Gregory Tripathi, Anoop Singh, Pallavi Jones, Maximillian R.W. Nanda, Amrit K. Musseau, Constance Craze, Melanie Bowden, Sarah Walker, Joseph F. Bentley, Alison R. Melnyk, Charles W. Hibberd, Julian M. |
| author_browse | Bentley, Alison R. Bowden, Sarah Craze, Melanie Hibberd, Julian M. Jones, Maximillian R.W. Melnyk, Charles W. Musseau, Constance Nanda, Amrit K. Reeves, Gregory Singh, Pallavi Tripathi, Anoop Walker, Joseph F. |
| author_facet | Reeves, Gregory Tripathi, Anoop Singh, Pallavi Jones, Maximillian R.W. Nanda, Amrit K. Musseau, Constance Craze, Melanie Bowden, Sarah Walker, Joseph F. Bentley, Alison R. Melnyk, Charles W. Hibberd, Julian M. |
| author_sort | Reeves, Gregory |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Grafting is possible in both animals and plants. Although in animals the process requires surgery and is often associated with rejection of non-self, in plants grafting is widespread, and has been used since antiquity for crop improvement1. However, in the monocotyledons, which represent the second largest group of terrestrial plants and include many staple crops, the absence of vascular cambium is thought to preclude grafting2. Here we show that the embryonic hypocotyl allows intra- and inter-specific grafting in all three monocotyledon groups: the commelinids, lilioids and alismatids. We show functional graft unions through histology, application of exogenous fluorescent dyes, complementation assays for movement of endogenous hormones, and growth of plants to maturity. Expression profiling identifies genes that unify the molecular response associated with grafting in monocotyledons and dicotyledons, but also gene families that have not previously been associated with tissue union. Fusion of susceptible wheat scions to oat rootstocks confers resistance to the soil-borne pathogen Gaeumannomyces graminis. Collectively, these data overturn the consensus that monocotyledons cannot form graft unions, and identify the hypocotyl (mesocotyl in grasses) as a meristematic tissue that allows this process. We conclude that graft compatibility is a shared ability among seed-bearing plants. |
| format | Journal Article |
| id | CGSpace129040 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1290402024-08-27T10:36:14Z Monocotyledonous plants graft at the embryonic root–shoot interface Reeves, Gregory Tripathi, Anoop Singh, Pallavi Jones, Maximillian R.W. Nanda, Amrit K. Musseau, Constance Craze, Melanie Bowden, Sarah Walker, Joseph F. Bentley, Alison R. Melnyk, Charles W. Hibberd, Julian M. plant physiology grafting monocotyledons Grafting is possible in both animals and plants. Although in animals the process requires surgery and is often associated with rejection of non-self, in plants grafting is widespread, and has been used since antiquity for crop improvement1. However, in the monocotyledons, which represent the second largest group of terrestrial plants and include many staple crops, the absence of vascular cambium is thought to preclude grafting2. Here we show that the embryonic hypocotyl allows intra- and inter-specific grafting in all three monocotyledon groups: the commelinids, lilioids and alismatids. We show functional graft unions through histology, application of exogenous fluorescent dyes, complementation assays for movement of endogenous hormones, and growth of plants to maturity. Expression profiling identifies genes that unify the molecular response associated with grafting in monocotyledons and dicotyledons, but also gene families that have not previously been associated with tissue union. Fusion of susceptible wheat scions to oat rootstocks confers resistance to the soil-borne pathogen Gaeumannomyces graminis. Collectively, these data overturn the consensus that monocotyledons cannot form graft unions, and identify the hypocotyl (mesocotyl in grasses) as a meristematic tissue that allows this process. We conclude that graft compatibility is a shared ability among seed-bearing plants. 2022-02-10 2023-02-26T12:49:20Z 2023-02-26T12:49:20Z Journal Article https://hdl.handle.net/10568/129040 en Limited Access Springer Reeves, G., Tripathi, A., Singh, P., Jones, M.R. W., Nanda, A.K., Musseau, C., Craze, M., Bowden, S., Walker, J.F., Bentley, A.R., Melnyk, C.W. and Hibberd, J.M. 2022. Monocotyledonous plants graft at the embryonic root–shoot interface. Nature 602(7896):280–286 |
| spellingShingle | plant physiology grafting monocotyledons Reeves, Gregory Tripathi, Anoop Singh, Pallavi Jones, Maximillian R.W. Nanda, Amrit K. Musseau, Constance Craze, Melanie Bowden, Sarah Walker, Joseph F. Bentley, Alison R. Melnyk, Charles W. Hibberd, Julian M. Monocotyledonous plants graft at the embryonic root–shoot interface |
| title | Monocotyledonous plants graft at the embryonic root–shoot interface |
| title_full | Monocotyledonous plants graft at the embryonic root–shoot interface |
| title_fullStr | Monocotyledonous plants graft at the embryonic root–shoot interface |
| title_full_unstemmed | Monocotyledonous plants graft at the embryonic root–shoot interface |
| title_short | Monocotyledonous plants graft at the embryonic root–shoot interface |
| title_sort | monocotyledonous plants graft at the embryonic root shoot interface |
| topic | plant physiology grafting monocotyledons |
| url | https://hdl.handle.net/10568/129040 |
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