Comprehending the evolution of gene editing platforms for crop trait improvement
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system was initially discovered as an underlying mechanism for conferring adaptive immunity to bacteria and archaea against viruses. Over the past decade, this has been repurposed as a genome-editing tool. Num...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Frontiers Media
2022
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| Online Access: | https://hdl.handle.net/10568/129039 |
| _version_ | 1855536515583574016 |
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| author | Dhakate, Priyanka Sehgal, Deepmala Vaishnavi, Samantha Chandra, Atika Singh, Apekshita Raina, Soom Nath Rajpal, Vijay Rani |
| author_browse | Chandra, Atika Dhakate, Priyanka Raina, Soom Nath Rajpal, Vijay Rani Sehgal, Deepmala Singh, Apekshita Vaishnavi, Samantha |
| author_facet | Dhakate, Priyanka Sehgal, Deepmala Vaishnavi, Samantha Chandra, Atika Singh, Apekshita Raina, Soom Nath Rajpal, Vijay Rani |
| author_sort | Dhakate, Priyanka |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system was initially discovered as an underlying mechanism for conferring adaptive immunity to bacteria and archaea against viruses. Over the past decade, this has been repurposed as a genome-editing tool. Numerous gene editing-based crop improvement technologies involving CRISPR/Cas platforms individually or in combination with next-generation sequencing methods have been developed that have revolutionized plant genome-editing methodologies. Initially, CRISPR/Cas nucleases replaced the earlier used sequence-specific nucleases (SSNs), such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), to address the problem of associated off-targets. The adaptation of this platform led to the development of concepts such as epigenome editing, base editing, and prime editing. Epigenome editing employed epi-effectors to manipulate chromatin structure, while base editing uses base editors to engineer precise changes for trait improvement. Newer technologies such as prime editing have now been developed as a “search-and-replace” tool to engineer all possible single-base changes. Owing to the availability of these, the field of genome editing has evolved rapidly to develop crop plants with improved traits. In this review, we present the evolution of the CRISPR/Cas system into new-age methods of genome engineering across various plant species and the impact they have had on tweaking plant genomes and associated outcomes on crop improvement initiatives. |
| format | Journal Article |
| id | CGSpace129039 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1290392025-12-08T10:29:22Z Comprehending the evolution of gene editing platforms for crop trait improvement Dhakate, Priyanka Sehgal, Deepmala Vaishnavi, Samantha Chandra, Atika Singh, Apekshita Raina, Soom Nath Rajpal, Vijay Rani crispr abiotic stress arabidopsis crop improvement dna electroporation gene editing rice wheat CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system was initially discovered as an underlying mechanism for conferring adaptive immunity to bacteria and archaea against viruses. Over the past decade, this has been repurposed as a genome-editing tool. Numerous gene editing-based crop improvement technologies involving CRISPR/Cas platforms individually or in combination with next-generation sequencing methods have been developed that have revolutionized plant genome-editing methodologies. Initially, CRISPR/Cas nucleases replaced the earlier used sequence-specific nucleases (SSNs), such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), to address the problem of associated off-targets. The adaptation of this platform led to the development of concepts such as epigenome editing, base editing, and prime editing. Epigenome editing employed epi-effectors to manipulate chromatin structure, while base editing uses base editors to engineer precise changes for trait improvement. Newer technologies such as prime editing have now been developed as a “search-and-replace” tool to engineer all possible single-base changes. Owing to the availability of these, the field of genome editing has evolved rapidly to develop crop plants with improved traits. In this review, we present the evolution of the CRISPR/Cas system into new-age methods of genome engineering across various plant species and the impact they have had on tweaking plant genomes and associated outcomes on crop improvement initiatives. 2022-08-23 2023-02-26T12:49:17Z 2023-02-26T12:49:17Z Journal Article https://hdl.handle.net/10568/129039 en Open Access application/pdf Frontiers Media Dhakate, P., Sehgal, D., Vaishnavi, S., Chandra, A., Singh, A., Raina, S. N., & Rajpal, V. R. (2022). Comprehending the evolution of gene editing platforms for crop trait improvement. Frontiers in Genetics, 13. https://doi.org/10.3389/fgene.2022.876987 |
| spellingShingle | crispr abiotic stress arabidopsis crop improvement dna electroporation gene editing rice wheat Dhakate, Priyanka Sehgal, Deepmala Vaishnavi, Samantha Chandra, Atika Singh, Apekshita Raina, Soom Nath Rajpal, Vijay Rani Comprehending the evolution of gene editing platforms for crop trait improvement |
| title | Comprehending the evolution of gene editing platforms for crop trait improvement |
| title_full | Comprehending the evolution of gene editing platforms for crop trait improvement |
| title_fullStr | Comprehending the evolution of gene editing platforms for crop trait improvement |
| title_full_unstemmed | Comprehending the evolution of gene editing platforms for crop trait improvement |
| title_short | Comprehending the evolution of gene editing platforms for crop trait improvement |
| title_sort | comprehending the evolution of gene editing platforms for crop trait improvement |
| topic | crispr abiotic stress arabidopsis crop improvement dna electroporation gene editing rice wheat |
| url | https://hdl.handle.net/10568/129039 |
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