Genetic diversity and population structure of Zymoseptoria tritici on bread wheat in Tunisia using SSR markers
Septoria tritici blotch (STB) caused by Zymoseptoria tritici is the primary biotic stress for durum wheat production in Tunisia. The present study was aimed to decipher the genetic diversity and population structure of Z. tritici bread wheat isolates; in the northern regions of Tunisia this pathogen...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2022
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| Online Access: | https://hdl.handle.net/10568/129038 |
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| author | Bel Hadj Chedli, Rim Aouini, Lamia Ben M’Barek, Sarrah Bahri, Bochra Verstappen, Els Kema Gerrit, H.J. Rezgui, Salah Yahyaoui, Amor Hassine Chaabane, Hanène |
| author_browse | Aouini, Lamia Bahri, Bochra Bel Hadj Chedli, Rim Ben M’Barek, Sarrah Chaabane, Hanène Kema Gerrit, H.J. Rezgui, Salah Verstappen, Els Yahyaoui, Amor Hassine |
| author_facet | Bel Hadj Chedli, Rim Aouini, Lamia Ben M’Barek, Sarrah Bahri, Bochra Verstappen, Els Kema Gerrit, H.J. Rezgui, Salah Yahyaoui, Amor Hassine Chaabane, Hanène |
| author_sort | Bel Hadj Chedli, Rim |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Septoria tritici blotch (STB) caused by Zymoseptoria tritici is the primary biotic stress for durum wheat production in Tunisia. The present study was aimed to decipher the genetic diversity and population structure of Z. tritici bread wheat isolates; in the northern regions of Tunisia this pathogen infected exclusively bread wheat. A total of 162 single-spore isolates were sampled during the 2015–2016 growing season from nine naturally infected bread wheat fields at four locations in the three main wheat-growing regions in Tunisia, previously reported as hot spots for Septoria disease (Cap Bon, Bizerte, and Beja). Collected isolates were fingerprinted using twelve polymorphic microsatellite markers to assess the genetic diversity and population structure of Z. tritici. All the microsatellite loci were polymorphic and a high genetic diversity was observed within the whole population. The highest Nei’s index value (0.42), Shannon Index (0.84) and private allele numbers (36) were found at the El Haouaria location (Cap Bon region). Furthermore, a moderate genetic differentiation within and among the surveyed locations (73% and 27%) was confirmed by analysis of molecular variance (AMOVA). On the other hand, the STRUCTURE program was shown to be less sensitive in revealing genetic structure especially at low levels of diversity. Thus, information on the genetic structure of the pathogen population collected from bread wheat is useful for designing and implementing durable and effective management strategies. |
| format | Journal Article |
| id | CGSpace129038 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1290382024-11-07T09:52:33Z Genetic diversity and population structure of Zymoseptoria tritici on bread wheat in Tunisia using SSR markers Bel Hadj Chedli, Rim Aouini, Lamia Ben M’Barek, Sarrah Bahri, Bochra Verstappen, Els Kema Gerrit, H.J. Rezgui, Salah Yahyaoui, Amor Hassine Chaabane, Hanène soft wheat genetic diversity population structure biotic stress wheat Septoria tritici blotch (STB) caused by Zymoseptoria tritici is the primary biotic stress for durum wheat production in Tunisia. The present study was aimed to decipher the genetic diversity and population structure of Z. tritici bread wheat isolates; in the northern regions of Tunisia this pathogen infected exclusively bread wheat. A total of 162 single-spore isolates were sampled during the 2015–2016 growing season from nine naturally infected bread wheat fields at four locations in the three main wheat-growing regions in Tunisia, previously reported as hot spots for Septoria disease (Cap Bon, Bizerte, and Beja). Collected isolates were fingerprinted using twelve polymorphic microsatellite markers to assess the genetic diversity and population structure of Z. tritici. All the microsatellite loci were polymorphic and a high genetic diversity was observed within the whole population. The highest Nei’s index value (0.42), Shannon Index (0.84) and private allele numbers (36) were found at the El Haouaria location (Cap Bon region). Furthermore, a moderate genetic differentiation within and among the surveyed locations (73% and 27%) was confirmed by analysis of molecular variance (AMOVA). On the other hand, the STRUCTURE program was shown to be less sensitive in revealing genetic structure especially at low levels of diversity. Thus, information on the genetic structure of the pathogen population collected from bread wheat is useful for designing and implementing durable and effective management strategies. 2022-06 2023-02-26T12:49:12Z 2023-02-26T12:49:12Z Journal Article https://hdl.handle.net/10568/129038 en Limited Access Springer Chedli, R.B.H., Aouini, L., M’Barek, S.B., Bahri, B.A., Verstappen, E., Kema Gerrit, H.J., Rezgui, S., Yahyaoui, A. and Chaabane, H. 2023. Genetic diversity and population structure of Zymoseptoria tritici on bread wheat in Tunisia using SSR markers. European Journal of Plant Pathology 163(2):429–440. https://doi.org/10.1007/s10658-022-02486-x |
| spellingShingle | soft wheat genetic diversity population structure biotic stress wheat Bel Hadj Chedli, Rim Aouini, Lamia Ben M’Barek, Sarrah Bahri, Bochra Verstappen, Els Kema Gerrit, H.J. Rezgui, Salah Yahyaoui, Amor Hassine Chaabane, Hanène Genetic diversity and population structure of Zymoseptoria tritici on bread wheat in Tunisia using SSR markers |
| title | Genetic diversity and population structure of Zymoseptoria tritici on bread wheat in Tunisia using SSR markers |
| title_full | Genetic diversity and population structure of Zymoseptoria tritici on bread wheat in Tunisia using SSR markers |
| title_fullStr | Genetic diversity and population structure of Zymoseptoria tritici on bread wheat in Tunisia using SSR markers |
| title_full_unstemmed | Genetic diversity and population structure of Zymoseptoria tritici on bread wheat in Tunisia using SSR markers |
| title_short | Genetic diversity and population structure of Zymoseptoria tritici on bread wheat in Tunisia using SSR markers |
| title_sort | genetic diversity and population structure of zymoseptoria tritici on bread wheat in tunisia using ssr markers |
| topic | soft wheat genetic diversity population structure biotic stress wheat |
| url | https://hdl.handle.net/10568/129038 |
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