In vitro propagation from nodal segments of Lippia origanoides (chemotype A)
This research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supple...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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FapUNIFESP
2022
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| Online Access: | https://hdl.handle.net/10568/128769 |
| _version_ | 1855529566049665024 |
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| author | Ramírez Moreno, Julián Sebastián Vega Porras, Sergio Andrés Escobar Pérez, Roosevelt Humberto Stashenko, Elena E. Fuentes Lorenzo, Jorge Luis Garzon Gutierrez, Luz Nayibe |
| author_browse | Escobar Pérez, Roosevelt Humberto Fuentes Lorenzo, Jorge Luis Garzon Gutierrez, Luz Nayibe Ramírez Moreno, Julián Sebastián Stashenko, Elena E. Vega Porras, Sergio Andrés |
| author_facet | Ramírez Moreno, Julián Sebastián Vega Porras, Sergio Andrés Escobar Pérez, Roosevelt Humberto Stashenko, Elena E. Fuentes Lorenzo, Jorge Luis Garzon Gutierrez, Luz Nayibe |
| author_sort | Ramírez Moreno, Julián Sebastián |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supplemented with different concentrations of gibberellic acid (GA), benzylaminopurine (BAP) and 1-naphthalenacetic acid (NAA) with BAP. The callus induction, shoots length, shoots number and root length, were analyzed. The treatments showed high percentage of callus formation at 0.5 to 1.5 mg L-1 of BAP alone or in combination with NAA (0.1 mg L-1). The highest value of shoot number per nodal segments was obtained at 1.5 mg L-1 of BAP (4.3 ± 0.8). The obtained plantlets were better rooted in vitro in the absence of plant growth regulators (PGRs) and they showed acclimatization rate of 90%. We reported a protocol for in vitro propagation and acclimatization of L. origanoides for A chemotypes from Colombia. |
| format | Journal Article |
| id | CGSpace128769 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | FapUNIFESP |
| publisherStr | FapUNIFESP |
| record_format | dspace |
| spelling | CGSpace1287692025-11-11T18:53:28Z In vitro propagation from nodal segments of Lippia origanoides (chemotype A) Ramírez Moreno, Julián Sebastián Vega Porras, Sergio Andrés Escobar Pérez, Roosevelt Humberto Stashenko, Elena E. Fuentes Lorenzo, Jorge Luis Garzon Gutierrez, Luz Nayibe micropropagation shoots nodes protocols lippia plant propagation This research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supplemented with different concentrations of gibberellic acid (GA), benzylaminopurine (BAP) and 1-naphthalenacetic acid (NAA) with BAP. The callus induction, shoots length, shoots number and root length, were analyzed. The treatments showed high percentage of callus formation at 0.5 to 1.5 mg L-1 of BAP alone or in combination with NAA (0.1 mg L-1). The highest value of shoot number per nodal segments was obtained at 1.5 mg L-1 of BAP (4.3 ± 0.8). The obtained plantlets were better rooted in vitro in the absence of plant growth regulators (PGRs) and they showed acclimatization rate of 90%. We reported a protocol for in vitro propagation and acclimatization of L. origanoides for A chemotypes from Colombia. 2022-07 2023-02-20T14:38:00Z 2023-02-20T14:38:00Z Journal Article https://hdl.handle.net/10568/128769 en Open Access application/pdf FapUNIFESP Ramírez Moreno, J.S.; Vega Porras, S.A.; Escobar, R.H.; Stashenko, E.E.; Fuentes Lorenzo, J.L.; Garzón Gutiérrez, L.N. (2022) In vitro propagation from nodal segments of Lippia origanoides (chemotype A). Ciência Rural, 52(7): e20210323. ISSN: 1678-4596 |
| spellingShingle | micropropagation shoots nodes protocols lippia plant propagation Ramírez Moreno, Julián Sebastián Vega Porras, Sergio Andrés Escobar Pérez, Roosevelt Humberto Stashenko, Elena E. Fuentes Lorenzo, Jorge Luis Garzon Gutierrez, Luz Nayibe In vitro propagation from nodal segments of Lippia origanoides (chemotype A) |
| title | In vitro propagation from nodal segments of Lippia origanoides (chemotype A) |
| title_full | In vitro propagation from nodal segments of Lippia origanoides (chemotype A) |
| title_fullStr | In vitro propagation from nodal segments of Lippia origanoides (chemotype A) |
| title_full_unstemmed | In vitro propagation from nodal segments of Lippia origanoides (chemotype A) |
| title_short | In vitro propagation from nodal segments of Lippia origanoides (chemotype A) |
| title_sort | in vitro propagation from nodal segments of lippia origanoides chemotype a |
| topic | micropropagation shoots nodes protocols lippia plant propagation |
| url | https://hdl.handle.net/10568/128769 |
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