Potential of Cameroon-indigenous isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae as microbial control agents of the flea beetle Nisotra uniformis
The flea beetle Nisotra uniformis Jacoby is a widespread pest of malvaceous crops for which synthetic chemical insecticides remain the principal control tool, particularly in sub-Saharan Africa. With the ultimate aim of developing a microbial alternative for N. uniformis control, we conducted labora...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Informa UK Limited
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/128732 |
| _version_ | 1855528683381456896 |
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| author | Niyibizi, I.A.B. Hanna, R. Kekeunou, S. Membang, G. Fiaboe, K. Mahot, H.C. Abang, A.F. Kumar, P. Lava Fotso Kuate, A. |
| author_browse | Abang, A.F. Fiaboe, K. Fotso Kuate, A. Hanna, R. Kekeunou, S. Kumar, P. Lava Mahot, H.C. Membang, G. Niyibizi, I.A.B. |
| author_facet | Niyibizi, I.A.B. Hanna, R. Kekeunou, S. Membang, G. Fiaboe, K. Mahot, H.C. Abang, A.F. Kumar, P. Lava Fotso Kuate, A. |
| author_sort | Niyibizi, I.A.B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The flea beetle Nisotra uniformis Jacoby is a widespread pest of malvaceous crops for which synthetic chemical insecticides remain the principal control tool, particularly in sub-Saharan Africa. With the ultimate aim of developing a microbial alternative for N. uniformis control, we conducted laboratory experiments (25 ± 1°C; 70 - 80% RH) to evaluate the biological attributes of three Cameroonian isolates of the entomopathogenic fungi Beauveria bassiana (Balsamo-Crivelli) Vuillemin and Metarhizium anisopliae sensu lato (Metchnikoff) Sorokin. Spore germination rates of the isolates varied from 0.5% after 4 hrs to 100% after 24 hrs on PDA medium, with MIITAC11.3.4 spores germinating fastest. Pathogenicity tests consisted of dipping adult beetles in conidial suspensions. All isolates were pathogenic to N. uniformis, with corrected mortalities varying between 23.3 - 86.7%. BIITAC-O-2 had the lowest LC50 at 5.17 × 106 conidia/ml, while MIITAC11.3.4 and BIITAC6.2.2 had LC50 at 4.11 × 107 conidia/ml, and 2.00 × 108 conidia/ml, respectively. BIITAC6.2.2 produced the shortest time to the highest mortality rate (1.17 days) and the shortest LT50 of 1.64 days but only at the highest concentration, followed by the two highest concentrations of MIITAC11.3.4 (LT50 at 3.43 and 5.70 days). The c parameter in the Weibull model showed that BIITAC-O-2 caused the highest rate of increase in mortality (2.42) at the highest concentration, followed by MIITAC11.3.4 (1.09) and BIITAC6.2.2 (0.50). The results of our study provide the basis for including N. uniformis in the ongoing development of BIITAC6.2.2 and MIITAC11.3.4 into biopesticide while continuing with further laboratory studies on BIITAC-O-2. |
| format | Journal Article |
| id | CGSpace128732 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace1287322024-11-07T09:52:16Z Potential of Cameroon-indigenous isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae as microbial control agents of the flea beetle Nisotra uniformis Niyibizi, I.A.B. Hanna, R. Kekeunou, S. Membang, G. Fiaboe, K. Mahot, H.C. Abang, A.F. Kumar, P. Lava Fotso Kuate, A. biological control okras pathogenicity spore germination cameroon The flea beetle Nisotra uniformis Jacoby is a widespread pest of malvaceous crops for which synthetic chemical insecticides remain the principal control tool, particularly in sub-Saharan Africa. With the ultimate aim of developing a microbial alternative for N. uniformis control, we conducted laboratory experiments (25 ± 1°C; 70 - 80% RH) to evaluate the biological attributes of three Cameroonian isolates of the entomopathogenic fungi Beauveria bassiana (Balsamo-Crivelli) Vuillemin and Metarhizium anisopliae sensu lato (Metchnikoff) Sorokin. Spore germination rates of the isolates varied from 0.5% after 4 hrs to 100% after 24 hrs on PDA medium, with MIITAC11.3.4 spores germinating fastest. Pathogenicity tests consisted of dipping adult beetles in conidial suspensions. All isolates were pathogenic to N. uniformis, with corrected mortalities varying between 23.3 - 86.7%. BIITAC-O-2 had the lowest LC50 at 5.17 × 106 conidia/ml, while MIITAC11.3.4 and BIITAC6.2.2 had LC50 at 4.11 × 107 conidia/ml, and 2.00 × 108 conidia/ml, respectively. BIITAC6.2.2 produced the shortest time to the highest mortality rate (1.17 days) and the shortest LT50 of 1.64 days but only at the highest concentration, followed by the two highest concentrations of MIITAC11.3.4 (LT50 at 3.43 and 5.70 days). The c parameter in the Weibull model showed that BIITAC-O-2 caused the highest rate of increase in mortality (2.42) at the highest concentration, followed by MIITAC11.3.4 (1.09) and BIITAC6.2.2 (0.50). The results of our study provide the basis for including N. uniformis in the ongoing development of BIITAC6.2.2 and MIITAC11.3.4 into biopesticide while continuing with further laboratory studies on BIITAC-O-2. 2023-03-04 2023-02-20T09:22:58Z 2023-02-20T09:22:58Z Journal Article https://hdl.handle.net/10568/128732 en Limited Access Informa UK Limited Niyibizi, I.A.B., Hanna, R., Kekeunou, S., Membang, G., Fiaboe, K., Mahot, H.C., ... & Fotso Kuate, A. (2023). Potential of Cameroon-indigenous isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae as microbial control agents of the flea beetle Nisotra uniformis. Biocontrol Science and Technology, 1-15. |
| spellingShingle | biological control okras pathogenicity spore germination cameroon Niyibizi, I.A.B. Hanna, R. Kekeunou, S. Membang, G. Fiaboe, K. Mahot, H.C. Abang, A.F. Kumar, P. Lava Fotso Kuate, A. Potential of Cameroon-indigenous isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae as microbial control agents of the flea beetle Nisotra uniformis |
| title | Potential of Cameroon-indigenous isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae as microbial control agents of the flea beetle Nisotra uniformis |
| title_full | Potential of Cameroon-indigenous isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae as microbial control agents of the flea beetle Nisotra uniformis |
| title_fullStr | Potential of Cameroon-indigenous isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae as microbial control agents of the flea beetle Nisotra uniformis |
| title_full_unstemmed | Potential of Cameroon-indigenous isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae as microbial control agents of the flea beetle Nisotra uniformis |
| title_short | Potential of Cameroon-indigenous isolates of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae as microbial control agents of the flea beetle Nisotra uniformis |
| title_sort | potential of cameroon indigenous isolates of the entomopathogenic fungi beauveria bassiana and metarhizium anisopliae as microbial control agents of the flea beetle nisotra uniformis |
| topic | biological control okras pathogenicity spore germination cameroon |
| url | https://hdl.handle.net/10568/128732 |
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