Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach
Pearl millet (Pennisetum glaucum L.), an important source of iron (Fe) and zinc (Zn) for millions of families in dryland tropics, helps in eradicating micronutrient malnutrition. The crop is rich in Fe and Zn, therefore, identification of the key genes operating the mineral pathways is an important...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Frontiers Media
2022
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| Acceso en línea: | https://hdl.handle.net/10568/128721 |
| _version_ | 1855521513579479040 |
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| author | Goud, Chengeshpur Anjali Satturu, Vanisri Malipatil, Renuka Viswanath, Aswini Semalaiyappan, Janani Kudapa, Himabindu Rathod, Santosha Rathore, Abhishek Govindaraj, Mahalingam Thirunavukkarasu, Nepolean |
| author_browse | Goud, Chengeshpur Anjali Govindaraj, Mahalingam Kudapa, Himabindu Malipatil, Renuka Rathod, Santosha Rathore, Abhishek Satturu, Vanisri Semalaiyappan, Janani Thirunavukkarasu, Nepolean Viswanath, Aswini |
| author_facet | Goud, Chengeshpur Anjali Satturu, Vanisri Malipatil, Renuka Viswanath, Aswini Semalaiyappan, Janani Kudapa, Himabindu Rathod, Santosha Rathore, Abhishek Govindaraj, Mahalingam Thirunavukkarasu, Nepolean |
| author_sort | Goud, Chengeshpur Anjali |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pearl millet (Pennisetum glaucum L.), an important source of iron (Fe) and zinc (Zn) for millions of families in dryland tropics, helps in eradicating micronutrient malnutrition. The crop is rich in Fe and Zn, therefore, identification of the key genes operating the mineral pathways is an important step to accelerate the development of biofortified cultivars. In a first-of-its kind experiment, leaf and root samples of a pearl millet inbred ICMB 1505 were exposed to combinations of Fe and Zn stress conditions using the
hydroponics method, and a whole-genome transcriptome assay was carried out to characterize the differentially expressed genes (DEGs) and pathways. A total of 37,093 DEGs under different combinations of stress conditions were identified, of which, 7,023 and 9,996 DEGs were reported in the leaf and root stress treatments, respectively. Among the 10,194 unique DEGs, 8,605
were annotated to cellular, biological, and molecular functions and 458 DEGs were assigned to 39 pathways. The results revealed the expression of major genes related to the mugineic acid pathway, phytohormones, chlorophyll biosynthesis, photosynthesis, and carbohydrate metabolism during Fe and Zn stress. The cross-talks between the Fe and Zn provided information on their
dual and opposite regulation of key uptake and transporter genes under Fe and Zn deficiency. SNP haplotypes in rice, maize, sorghum, and foxtail millet as well as in Arabidopsis using pearl millet Fe and Zn responsive genes could be used for designing the markers in staple crops. Our results will assist in developing Fe and Zn-efficient pearl millet varieties in biofortification breeding
programs and precision delivery mechanisms to ameliorate malnutrition in South Asia and Sub-Saharan Africa. |
| format | Journal Article |
| id | CGSpace128721 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1287212025-12-08T10:29:22Z Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach Goud, Chengeshpur Anjali Satturu, Vanisri Malipatil, Renuka Viswanath, Aswini Semalaiyappan, Janani Kudapa, Himabindu Rathod, Santosha Rathore, Abhishek Govindaraj, Mahalingam Thirunavukkarasu, Nepolean malnutrition biofortification genes iron zinc malnutrición bioenriquecimiento Pearl millet (Pennisetum glaucum L.), an important source of iron (Fe) and zinc (Zn) for millions of families in dryland tropics, helps in eradicating micronutrient malnutrition. The crop is rich in Fe and Zn, therefore, identification of the key genes operating the mineral pathways is an important step to accelerate the development of biofortified cultivars. In a first-of-its kind experiment, leaf and root samples of a pearl millet inbred ICMB 1505 were exposed to combinations of Fe and Zn stress conditions using the hydroponics method, and a whole-genome transcriptome assay was carried out to characterize the differentially expressed genes (DEGs) and pathways. A total of 37,093 DEGs under different combinations of stress conditions were identified, of which, 7,023 and 9,996 DEGs were reported in the leaf and root stress treatments, respectively. Among the 10,194 unique DEGs, 8,605 were annotated to cellular, biological, and molecular functions and 458 DEGs were assigned to 39 pathways. The results revealed the expression of major genes related to the mugineic acid pathway, phytohormones, chlorophyll biosynthesis, photosynthesis, and carbohydrate metabolism during Fe and Zn stress. The cross-talks between the Fe and Zn provided information on their dual and opposite regulation of key uptake and transporter genes under Fe and Zn deficiency. SNP haplotypes in rice, maize, sorghum, and foxtail millet as well as in Arabidopsis using pearl millet Fe and Zn responsive genes could be used for designing the markers in staple crops. Our results will assist in developing Fe and Zn-efficient pearl millet varieties in biofortification breeding programs and precision delivery mechanisms to ameliorate malnutrition in South Asia and Sub-Saharan Africa. 2022-11-22 2023-02-16T09:29:10Z 2023-02-16T09:29:10Z Journal Article https://hdl.handle.net/10568/128721 en Open Access application/pdf Frontiers Media Goud, C.A.; Satturu, V.; Malipatil, R.; Viswanath, A.; Semalaiyappan, J.; Kudapa, H.; Rathod, Santosha; Rathore, Abhishek; Govindaraj, Mahalingam; Thirunavukkarasu, Nepolean (2022) Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach. Frontiers in Nutrition 9:884381 17 p. ISSN: 2296-861X |
| spellingShingle | malnutrition biofortification genes iron zinc malnutrición bioenriquecimiento Goud, Chengeshpur Anjali Satturu, Vanisri Malipatil, Renuka Viswanath, Aswini Semalaiyappan, Janani Kudapa, Himabindu Rathod, Santosha Rathore, Abhishek Govindaraj, Mahalingam Thirunavukkarasu, Nepolean Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach |
| title | Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach |
| title_full | Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach |
| title_fullStr | Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach |
| title_full_unstemmed | Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach |
| title_short | Identification of iron and zinc responsive genes in pearl millet using genome-wide RNA-sequencing approach |
| title_sort | identification of iron and zinc responsive genes in pearl millet using genome wide rna sequencing approach |
| topic | malnutrition biofortification genes iron zinc malnutrición bioenriquecimiento |
| url | https://hdl.handle.net/10568/128721 |
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