Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal
Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. However, the disease has not been...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/128495 |
| _version_ | 1855520299764678656 |
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| author | Pant, Bimala Bai, Tingting Du, Chanjuan Baidya, Suraj Magar, Prem Bahadur Manandhar, Shrinkhala Shrestha, Jiban Dita Rodriguez, Miguel Angel Rouard, Mathieu Fu, Gang Zheng, Sijun |
| author_browse | Bai, Tingting Baidya, Suraj Dita Rodriguez, Miguel Angel Du, Chanjuan Fu, Gang Magar, Prem Bahadur Manandhar, Shrinkhala Pant, Bimala Rouard, Mathieu Shrestha, Jiban Zheng, Sijun |
| author_facet | Pant, Bimala Bai, Tingting Du, Chanjuan Baidya, Suraj Magar, Prem Bahadur Manandhar, Shrinkhala Shrestha, Jiban Dita Rodriguez, Miguel Angel Rouard, Mathieu Fu, Gang Zheng, Sijun |
| author_sort | Pant, Bimala |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. However, the disease has not been officially reported yet, and consequently, little is known about the pathogen present across the country. In this study, we characterized 13 fungal strains isolated from banana plants of the Malbhog cultivar (Silk, AAB) showing symptoms similar to FWB in banana plantations in Nepal. All of the strains were typed as belonging to the F. oxysporum and caused FWB symptoms when inoculated in the Malbhog and Cachaco (Bluggoe, ABB) cultivars. No symptoms were observed in the Williams cultivar (Cavendish, AAA). Vegetative compatibility group (VCG) analysis classified the strains as VCG 0124 or VCG 0125. PCR analyses conducted with primers specific for Foc race 1 (Foc R1) or Foc tropical race 4 (TR4) revealed that all the strains reacted positively for Foc R1 and none for TR4. Altogether, our results demonstrated that the pathogen populations causing FWB of the Malbhog cultivar in Nepal were Foc R1. This work reported, for the first time, the occurrence of FWB in Nepal. Further studies with larger Foc populations are needed to better understand disease epidemiology to design sustainable disease management strategies. |
| format | Journal Article |
| id | CGSpace128495 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1284952025-12-08T10:29:22Z Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal Pant, Bimala Bai, Tingting Du, Chanjuan Baidya, Suraj Magar, Prem Bahadur Manandhar, Shrinkhala Shrestha, Jiban Dita Rodriguez, Miguel Angel Rouard, Mathieu Fu, Gang Zheng, Sijun bananas fusarium oxysporum pathogenic fungi detection identification pathogens Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. However, the disease has not been officially reported yet, and consequently, little is known about the pathogen present across the country. In this study, we characterized 13 fungal strains isolated from banana plants of the Malbhog cultivar (Silk, AAB) showing symptoms similar to FWB in banana plantations in Nepal. All of the strains were typed as belonging to the F. oxysporum and caused FWB symptoms when inoculated in the Malbhog and Cachaco (Bluggoe, ABB) cultivars. No symptoms were observed in the Williams cultivar (Cavendish, AAA). Vegetative compatibility group (VCG) analysis classified the strains as VCG 0124 or VCG 0125. PCR analyses conducted with primers specific for Foc race 1 (Foc R1) or Foc tropical race 4 (TR4) revealed that all the strains reacted positively for Foc R1 and none for TR4. Altogether, our results demonstrated that the pathogen populations causing FWB of the Malbhog cultivar in Nepal were Foc R1. This work reported, for the first time, the occurrence of FWB in Nepal. Further studies with larger Foc populations are needed to better understand disease epidemiology to design sustainable disease management strategies. 2023-02-05 2023-02-07T16:43:21Z 2023-02-07T16:43:21Z Journal Article https://hdl.handle.net/10568/128495 en Open Access application/pdf MDPI Pant, B.; Bai, T.; Du, C.; Baidya, S.; Magar, P.B.; Manandhar, S.; Shrestha, J.; Dita, M.; Rouard, M.; Fu, G.; Zheng, S.-J. (2023) Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal. Journal of Fungi 9: 208. ISSN: 2309-608X |
| spellingShingle | bananas fusarium oxysporum pathogenic fungi detection identification pathogens Pant, Bimala Bai, Tingting Du, Chanjuan Baidya, Suraj Magar, Prem Bahadur Manandhar, Shrinkhala Shrestha, Jiban Dita Rodriguez, Miguel Angel Rouard, Mathieu Fu, Gang Zheng, Sijun Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal |
| title | Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal |
| title_full | Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal |
| title_fullStr | Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal |
| title_full_unstemmed | Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal |
| title_short | Molecular diagnosis and vegetative compatibility group analysis of Fusarium Wilt of banana in Nepal |
| title_sort | molecular diagnosis and vegetative compatibility group analysis of fusarium wilt of banana in nepal |
| topic | bananas fusarium oxysporum pathogenic fungi detection identification pathogens |
| url | https://hdl.handle.net/10568/128495 |
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